FlyBase Allele Report: Dmel\Cdc42[2]

General Information

Symbol

Dmel\Cdc42²

Species

D. melanogaster

Name

FlyBase ID

FBal0062065

Feature type

allele

Associated gene

Dmel\Cdc42

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Fehon et al., 1997)

Mutagen

ethyl methanesulfonate

(Fehon et al., 1997)

Progenitor genotype

Cytology

Description

Nucleotide substitution: T311A. Mutation in splice donor consensus site.

(Genova et al., 2000)

Mutation in the splice donor sequence of the small internal intron.

(Fehon et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:19,698,247..19,698,247 [+]

Nucleotide change:

T19698247A

Reported nucleotide change:

T311A

(Genova et al., 2000, Fehon et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

actin filament & rhabdomere | somatic clone

(Zelhof and Hardy, 2004)

adult mushroom body alpha-lobe | adult stage

(Dollar et al., 2016)

adult mushroom body beta-lobe | adult stage

(Dollar et al., 2016)

axon | adult stage

(Dollar et al., 2016)

bouton

(Nahm et al., 2010)

embryonic/larval neuromuscular junction | third instar larval stage

(Rodal et al., 2008)

follicle cell | somatic clone

(Fic et al., 2021)

neuromuscular junction

(Nahm et al., 2010)

NMJ bouton | third instar larval stage

(Rodal et al., 2008)

ommatidium | P-stage

(Gazsó-Gerhát et al., 2023)

Detailed Description

Statement

Reference

Cdc42²/Y males have mushroom body axon growth and guidance defects.

(Dollar et al., 2016)

The hatching rate of eggs derived from homozygous females is reduced compared to wild type, regardless of the zygotic state.

(Leibfried et al., 2013)

Mutant larvae fail to encapsulate eggs when parasitised by the avirulent L. boulardi wasp strain G486 (no melanisation is seen). Plasmatocytes and lamellocytes fully cover the egg.

(Howell et al., 2012)

Homozygous larvae fail to encapsulate eggs of the avirulent wasp strain L. boulardi G486.

Isolated plasmatocytes from homozygous larvae show a reduced ability to phagocytose E. coli and S. aureus in a phagocytosis assay.

(Sampson et al., 2012)

Cdc42² heterozygous larvae exhibit wild-type neuromuscular junction length and total bouton number, but satellite bouton formation is slightly elevated.

(Nahm et al., 2010)

Cdc42² third instar larvae exhibit synaptic overgrowth and an increase in bouton number in segment A3, muscle 6/7 compared to controls.

(Rodal et al., 2008)

Cdc42⁴/Cdc42² flies show normal resistance to infection with P.aeruginosa by septic injury.

(Avet-Rochex et al., 2007)

Cdc42² mutants exhibit a delay in F-actin enrichment and accumulation in rhabdomeres.

(Zelhof and Hardy, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cdc42² has abnormal neuroanatomy | P-stage phenotype, enhanceable by Frl⁵⁹

(Gazsó-Gerhát et al., 2023)

Cdc42² has abnormal neuroanatomy | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex83

(Dollar et al., 2016)

Cdc42² has abnormal neuroanatomy | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex88

(Dollar et al., 2016)

Cdc42² has abnormal neuroanatomy | adult stage phenotype, enhanceable by Frl[+]/Frl^ExK62

(Dollar et al., 2016)

Cdc42² has abnormal neuroanatomy phenotype, enhanceable by Cip4¹/Cip4[+]

(Nahm et al., 2010)

Cdc42² has abnormal neuroanatomy phenotype, enhanceable by Cip4¹/WASp[+]/WASp¹/Cip4[+]

(Nahm et al., 2010)

Cdc42² has abnormal size | third instar larval stage phenotype, enhanceable by nwk¹

(Rodal et al., 2008)

Cdc42² has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by nwk¹

(Rodal et al., 2008)

Cdc42² has abnormal size | third instar larval stage phenotype, enhanceable by nwk²

(Rodal et al., 2008)

Cdc42² has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by nwk²

(Rodal et al., 2008)

Cdc42² has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by nwk¹/nwk²

(Rodal et al., 2008)

Suppressed by

Statement

Reference

Cdc42², nwk² has abnormal size | third instar larval stage phenotype, suppressible by Cdc42^{Ubi-p63E.Tag:MYC}

(Rodal et al., 2008)

Enhancer of

Statement

Reference

Cdc42² is an enhancer of abnormal neuroanatomy | P-stage phenotype of DAAM^Ex4/DAAM^Ex68

(Gazsó-Gerhát et al., 2023)

Cdc42[+]/Cdc42² is an enhancer of abnormal neuroanatomy phenotype of Cip4¹

(Nahm et al., 2010)

Cdc42[+], WASp[+], WASp¹, Cdc42² is an enhancer of abnormal neuroanatomy phenotype of Cip4¹

(Nahm et al., 2010)

Cdc42[+], Cip4¹, Cip4[+], Cdc42² is an enhancer of abnormal neuroanatomy phenotype of WASp¹

(Nahm et al., 2010)

Cdc42² is an enhancer of abnormal size | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

Cdc42² is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

Cdc42² is an enhancer of abnormal size | third instar larval stage phenotype of nwk²

(Rodal et al., 2008)

Other

Statement

Reference

Cdc42[+]/Cdc42², Zir^BG00267 has abnormal immune response | dominant | larval stage phenotype

(Sampson et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cdc42² has ommatidium | P-stage phenotype, enhanceable by Frl⁵⁹

(Gazsó-Gerhát et al., 2023)

Cdc42² has axon | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex83

(Dollar et al., 2016)

Cdc42² has adult mushroom body beta-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex83

(Dollar et al., 2016)

Cdc42² has adult mushroom body alpha-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex83

(Dollar et al., 2016)

Cdc42² has axon | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex88

(Dollar et al., 2016)

Cdc42² has adult mushroom body beta-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex88

(Dollar et al., 2016)

Cdc42² has adult mushroom body alpha-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex88

(Dollar et al., 2016)

Cdc42² has axon | adult stage phenotype, enhanceable by Frl[+]/Frl^ExK62

(Dollar et al., 2016)

Cdc42² has adult mushroom body beta-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^ExK62

(Dollar et al., 2016)

Cdc42² has adult mushroom body alpha-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^ExK62

(Dollar et al., 2016)

Cdc42² has NMJ bouton phenotype, enhanceable by Cip4¹/Cip4[+]

(Nahm et al., 2010)

Cdc42² has neuromuscular junction phenotype, enhanceable by Cip4¹/Cip4[+]

(Nahm et al., 2010)

Cdc42² has NMJ bouton phenotype, enhanceable by Cip4¹/WASp[+]/WASp¹/Cip4[+]

(Nahm et al., 2010)

Cdc42² has neuromuscular junction phenotype, enhanceable by Cip4¹/WASp[+]/WASp¹/Cip4[+]

(Nahm et al., 2010)

Cdc42² has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by nwk¹

(Rodal et al., 2008)

Cdc42² has NMJ bouton | third instar larval stage phenotype, enhanceable by nwk¹

(Rodal et al., 2008)

Cdc42² has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by nwk²

(Rodal et al., 2008)

Cdc42² has NMJ bouton | third instar larval stage phenotype, enhanceable by nwk²

(Rodal et al., 2008)

Cdc42² has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by nwk¹/nwk²

(Rodal et al., 2008)

Cdc42² has NMJ bouton | third instar larval stage phenotype, enhanceable by nwk¹/nwk²

(Rodal et al., 2008)

Suppressed by

Statement

Reference

Cdc42², nwk² has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Cdc42^{Ubi-p63E.Tag:MYC}

(Rodal et al., 2008)

Cdc42², nwk² has NMJ bouton | third instar larval stage phenotype, suppressible by Cdc42^{Ubi-p63E.Tag:MYC}

(Rodal et al., 2008)

NOT suppressed by

Statement

Reference

Cdc42² has phenotype, non-suppressible by Mer^hs.PM

(Fehon et al., 1997)

Enhancer of

Statement

Reference

Cdc42² is an enhancer of ommatidium | P-stage phenotype of DAAM^Ex4/DAAM^Ex68

(Gazsó-Gerhát et al., 2023)

Cdc42[+]/Cdc42² is an enhancer of NMJ bouton phenotype of Cip4¹

(Nahm et al., 2010)

Cdc42[+]/Cdc42² is an enhancer of neuromuscular junction phenotype of Cip4¹

(Nahm et al., 2010)

Cdc42[+], WASp[+], WASp¹, Cdc42² is an enhancer of NMJ bouton phenotype of Cip4¹

(Nahm et al., 2010)

Cdc42[+], WASp[+], WASp¹, Cdc42² is an enhancer of neuromuscular junction phenotype of Cip4¹

(Nahm et al., 2010)

Cdc42[+], Cip4¹, Cip4[+], Cdc42² is an enhancer of NMJ bouton phenotype of WASp¹

(Nahm et al., 2010)

Cdc42[+], Cip4¹, Cip4[+], Cdc42² is an enhancer of neuromuscular junction phenotype of WASp¹

(Nahm et al., 2010)

Cdc42² is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

Cdc42² is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of nwk²

(Rodal et al., 2008)

Cdc42² is an enhancer of NMJ bouton | third instar larval stage phenotype of nwk²

(Rodal et al., 2008)

Cdc42[+]/Cdc42² is an enhancer of wing hair | increased number phenotype of Scer\GAL4^ap-md544, trc^T453A.UAS.L

(He et al., 2005)

Suppressor of

Statement

Reference

Cdc42² is a suppressor of NMJ bouton | third instar larval stage phenotype of nwk¹

(Rodal et al., 2008)

Cdc42[+]/Cdc42² is a suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

NOT Suppressor of

Statement

Reference

Cdc42[+]/Cdc42² is a non-suppressor of aster | embryonic cycle 5 phenotype of CycB^+t10

(Ji et al., 2002)

Cdc42[+]/Cdc42² is a non-suppressor of aster | embryonic cycle 6 phenotype of CycB^+t10

(Ji et al., 2002)

Cdc42[+]/Cdc42² is a non-suppressor of aster | embryonic cycle 7 phenotype of CycB^+t10

(Ji et al., 2002)

Cdc42[+]/Cdc42² is a non-suppressor of mitotic domain 1 | embryonic cycle 10 phenotype of CycB^+t10

(Ji et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of Frl^Ex83/+, Frl^Ex88/+ or Frl^ExK62/+ enhances the mushroom body axon growth defects seen in Cdc42²/Y flies.

(Dollar et al., 2016)

Zir^BG00267/+ ; Cdc42²/+ double heterozygous larvae have a reduced rate of encapsulation (37%) of eggs of the avirulent wasp strain L. boulardi G486 compared to the rate seen in either single heterozygote.

(Sampson et al., 2012)

Cdc42²; Cip4¹ double heterozygotes exhibit an increase in total bouton number, satellite bouton formation and neuromuscular junction length.

Cdc42²/+; Cip4¹/+; WASp¹/+ trans-heterozygous larvae exhibit an increase in total bouton number, neuromuscular junction length and in particular satellite bouton formation.

(Nahm et al., 2010)

Cdc42² nwk¹ double mutants exhibit more severe synaptic overgrowth and an increased number of boutons and satellite boutons compared to either single mutant.

Cdc42² nwk² double mutants exhibit more severe synaptic overgrowth and an increased number of boutons and satellite boutons compared to either single mutant.

Cdc42² nwk²/nwk¹ double mutants exhibit an increased number of satellite boutons in segment A2-A3, muscle 4 compared to either Cdc42² or nwk² homozygous single mutants.

Expression of Cdc42^{Ubi-p63E.T:Hsap\MYC} rescues the synaptic overgrowth and increased bouton and satellite bouton number phenotypes seen in nwk² Cdc42² double mutant larvae.

(Rodal et al., 2008)

The Cdc42² mutation fails to modify the cv-c^M62 phenotype in the Malpighian tubules.

(Denholm et al., 2005)

A Cdc42²/+ background enhances the splitting wing hair phenotype of flies that express trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ap-md544.

(He et al., 2005)

Not rescued by Mer^hs.PM.

(Fehon et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cdc42² is rescued by Cdc42^Ubi-p63E.PF

(Fehon et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9105

y¹ w^* Cdc42² P{neoFRT}19A

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF153425

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAD43789

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Cdc42 2

(Howell et al., 2012)

Cdc42²

(Gazsó-Gerhát et al., 2023, Dollar et al., 2016, Leibfried et al., 2013, Sampson et al., 2012, Frank et al., 2009)

Dcdc42²

(He et al., 2005)

cdc42²

(Fic et al., 2021, Matsuda et al., 2013, Nahm et al., 2010, Rodal et al., 2008, Avet-Rochex et al., 2007, Denholm et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (17)

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