FlyBase Allele Report: Dmel\nwk[2]

General Information

Symbol

Dmel\nwk²

Species

D. melanogaster

Name

FlyBase ID

FBal0154818

Feature type

allele

Associated gene

Dmel\nwk

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

gamma ray

Nature of the Allele

Allele class

Mutagen

gamma ray

(Coyle et al., 2004)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

nwk²/nwk² third instar larvae do not have significantly different miniature excitatory junction potential (mEJP) amplitude, but do have significant reduction in evoked EJP amplitude and quantal content (number of vesicles released per action potential), at the NMJ compared to controls.

(Ukken et al., 2016)

nwk¹/nwk² mutant third instar larvae show an increase in the total synaptic bouton number at the neuromuscular junction as well as an increase in the satellite boutons.

(Kelley et al., 2015)

The average number of boutons and of satellite boutons at the neuromuscular junction is increased in mutant larvae.

(Rodal et al., 2011)

Total bouton number and satellite bouton number at the neuromuscular junction is increased in nwk¹/nwk² larvae compared to controls.

(O'Connor-Giles et al., 2008)

nwk² third instar larvae display synaptic overgrowth and an increase in bouton number in segment A3, muscle 6/7 compared to wild type.

nwk¹/nwk² mutants exhibit an increase in overall bouton number.

Expression of nwk^{SH3ab.Scer\UAS} under the control of Scer\GAL4^elav-C155 enhances the synaptic overgrowth and increased bouton number phenotypes seen in nwk¹/nwk² third instar larvae.

(Rodal et al., 2008)

Homozygous and hemizygous larvae show an increase in bouton number/muscle area, at the neuromuscular junction (NMJ), an increase in NMJ length/muscle area and an increase in the number of branching boutons per NMJ compared to wild type. The general anatomy of type 1b boutons at neuromuscular junction 6/7 appears normal in mutant larvae; the size and abundance of synaptic vesicles appears normal and the distribution of T bars per synaptic contact is similar to wild type. However, the general shape of the boutons is abnormal in the mutant larvae; they are elliptical in cross section (compared to circular cross-sections in wild type) and the surface area and volume of the boutons are reduced (by 42% and 60% respectively) compared to wild type. The mutant boutons have 50% fewer synaptic contacts than wild-type boutons. The mean amplitude of the excitatory junctional current (EJC) at neuromuscular junction 6/7 is reduced by more than 50% compared to wild-type larvae. The amplitude of miniature EJCs (quantal size) is slightly increased compared to wild type. mEJC frequency is not significantly different from wild type. Heterozygotes show a low incidence of hyperbranching of boutons at the larval NMJ.

(Coyle et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

nwk¹/nwk² has abnormal neuroanatomy | larval stage phenotype, enhanceable by Dap160[+]/Dap160^Δ1

(O'Connor-Giles et al., 2008)

nwk¹/nwk² has abnormal neuroanatomy | larval stage phenotype, enhanceable by shi[+]/shi¹

(O'Connor-Giles et al., 2008)

nwk² has abnormal neuroanatomy | larval stage phenotype, enhanceable by Scer\GAL4^elav.PU/tkv^{Q199D.UAS.Tag:HA}

(O'Connor-Giles et al., 2008)

nwk¹/nwk² has abnormal neuroanatomy | larval stage phenotype, enhanceable by EndoA^EY02730/endoA[+]

(O'Connor-Giles et al., 2008)

nwk² has abnormal size | third instar larval stage phenotype, enhanceable by Cdc42²

(Rodal et al., 2008)

nwk² has abnormal neuroanatomy phenotype, enhanceable by WASp¹

(Coyle et al., 2004)

Suppressed by

Statement

Reference

nwk² has abnormal neuroanatomy | larval stage phenotype, suppressible by Snx16^e01600/Snx16^Δ2

(Rodal et al., 2011)

nwk² has abnormal neuroanatomy | larval stage phenotype, suppressible by arr^k08131/arr²

(Rodal et al., 2011)

nwk² has abnormal neuroanatomy | larval stage phenotype, suppressible by Snx16^Δ1/Snx16^e01600

(Rodal et al., 2011)

nwk¹/nwk² has abnormal neuroanatomy | larval stage phenotype, suppressible by wit^A12/wit[+]

(O'Connor-Giles et al., 2008)

Cdc42², nwk² has abnormal size | third instar larval stage phenotype, suppressible by Cdc42^{Ubi-p63E.Tag:MYC}

(Rodal et al., 2008)

NOT suppressed by

Statement

Reference

nwk² has abnormal neurophysiology | third instar larval stage phenotype, non-suppressible by SH3PX1^C1/SH3PX1^10A

(Ukken et al., 2016)

Enhancer of

Statement

Reference

nwk² is an enhancer of abnormal size | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk² is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk¹/nwk² is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk² is an enhancer of abnormal neuroanatomy phenotype of WASp¹

(Coyle et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

nwk¹/nwk² has NMJ bouton | larval stage | increased number phenotype, enhanceable by Dap160[+]/Dap160^Δ1

(O'Connor-Giles et al., 2008)

nwk¹/nwk² has NMJ bouton | larval stage | increased number phenotype, enhanceable by shi[+]/shi¹

(O'Connor-Giles et al., 2008)

nwk² has NMJ bouton | larval stage | increased number phenotype, enhanceable by Scer\GAL4^elav.PU/tkv^{Q199D.UAS.Tag:HA}

(O'Connor-Giles et al., 2008)

nwk¹/nwk² has NMJ bouton | larval stage | increased number phenotype, enhanceable by EndoA^EY02730/endoA[+]

(O'Connor-Giles et al., 2008)

nwk² has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by Cdc42²

(Rodal et al., 2008)

nwk² has NMJ bouton | third instar larval stage phenotype, enhanceable by Cdc42²

(Rodal et al., 2008)

nwk² has bouton | increased number phenotype, enhanceable by WASp¹

(Coyle et al., 2004)

nwk² has bouton phenotype, enhanceable by WASp¹

(Coyle et al., 2004)

nwk² has neuromuscular junction | larval stage phenotype, enhanceable by WASp¹

(Coyle et al., 2004)

Suppressed by

Statement

Reference

nwk² has NMJ bouton | increased number | larval stage phenotype, suppressible by Snx16^Δ1/Snx16^e01600

(Rodal et al., 2011)

nwk² has NMJ bouton | increased number | larval stage phenotype, suppressible by Snx16^e01600/Snx16^Δ2

(Rodal et al., 2011)

nwk² has NMJ bouton | increased number | larval stage phenotype, suppressible by arr^k08131/arr²

(Rodal et al., 2011)

nwk¹/nwk² has NMJ bouton | increased number | larval stage phenotype, suppressible by wit^A12/wit[+]

(O'Connor-Giles et al., 2008)

Cdc42², nwk² has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Cdc42^{Ubi-p63E.Tag:MYC}

(Rodal et al., 2008)

Cdc42², nwk² has NMJ bouton | third instar larval stage phenotype, suppressible by Cdc42^{Ubi-p63E.Tag:MYC}

(Rodal et al., 2008)

Enhancer of

Statement

Reference

nwk² is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk² is an enhancer of NMJ bouton | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk¹/nwk² is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk¹/nwk² is an enhancer of NMJ bouton | third instar larval stage phenotype of Cdc42²

(Rodal et al., 2008)

nwk² is an enhancer of bouton | increased number phenotype of WASp¹

(Coyle et al., 2004)

nwk² is an enhancer of bouton phenotype of WASp¹

(Coyle et al., 2004)

nwk² is an enhancer of neuromuscular junction | larval stage phenotype of WASp¹

(Coyle et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

SH3PX1^10A/SH3PX1^C1 nwk²/nwk² double mutants do not have significantly different quantal content deficits or further reduced evoked EJP amplitude compared to nwk²/nwk² mutants at the third instar larval neuromuscular junction (though there is a trend towards reduced quantal content and EJP amplitude).

(Ukken et al., 2016)

The increase in average bouton number and satellite bouton number at the neuromuscular junction which is seen in nwk² larvae is suppressed if they are also carrying either Snx16^Δ1/Snx16^e01600 or Snx16^Δ2/Snx16^e01600.

The increase in average satellite bouton number at the neuromuscular junction which is seen in nwk² larvae is suppressed if they are also carrying arr^k08131/arr².

(Rodal et al., 2011)

The increase in total bouton number and satellite bouton number at the neuromuscular junction that is seen in nwk¹/nwk² larvae is enhanced if the larvae are also carrying endoA^EY02730/+, Dap160^Δ1/+ or shi¹/+.

wit^A12/+ suppresses the increase in total bouton number at the neuromuscular junction that is seen in nwk¹/nwk² larvae.

Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PU enhances the increase in the average number of satellite boutons at the neuromuscular junction that is seen in nwk² larvae.

(O'Connor-Giles et al., 2008)

Cdc42² nwk² double mutants exhibit more severe synaptic overgrowth and an increased number of boutons and satellite boutons compared to either single mutant.

Expression of Cdc42^{Ubi-p63E.T:Hsap\MYC} rescues the synaptic overgrowth and increased bouton and satellite bouton number phenotypes seen in nwk² Cdc42² double mutant larvae.

(Rodal et al., 2008)

WASp¹ nwk² double mutant larvae display more severe neuromuscular junction (NMJ) phenotypes than either single mutant with respect to bouton number, branch formation and NMJ length. The complexity of hyperbranching at the NMJ is also dramatically increased in the double mutants.

(Coyle et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

nwk¹

(Coyle et al., 2004)

Rescued by

nwk¹/nwk² is rescued by Scer\GAL4^elav-C155/nwk^UAS.EGFP

(Kelley et al., 2015)

nwk² is rescued by Scer\GAL4^elav-C155/nwk^UAS.cCa

(Coyle et al., 2004)

Partially rescued by

nwk¹/nwk² is partially rescued by nwk^UAS.cCa

(Rodal et al., 2008)

nwk¹/nwk² is partially rescued by Scer\GAL4^elav-C155/nwk^ΔC.UAS

(Rodal et al., 2008)

nwk² is partially rescued by nwk^UAS.cCa/Scer\GAL4^elav.PLu

(Coyle et al., 2004)

Not rescued by

nwk¹/nwk² is not rescued by Scer\GAL4^elav-C155/nwk^{Δ1-428.UAS.EGFP}

(Kelley et al., 2015)

nwk¹/nwk² is not rescued by Scer\GAL4^elav-C155/nwk^{1-428.UAS.EGFP}

(Kelley et al., 2015)

nwk¹/nwk² is not rescued by Scer\GAL4^elav-C155/nwk^SH3a.UAS

(Rodal et al., 2008)

nwk¹/nwk² is not rescued by Scer\GAL4^elav-C155/nwk^SH3b.UAS

(Rodal et al., 2008)

nwk¹/nwk² is not rescued by nwk^SH3ab.UAS

(Rodal et al., 2008)

nwk² is not rescued by nwk^UAS.cCa/Scer\GAL4^C57

(Coyle et al., 2004)

Comments

The increased number of boutons at the neuromuscular junction observed in the nwk¹/nwk² mutant third instar larvae is rescued by expression of nwk^{Scer\UAS.T:Avic\GFP-EGFP}, is not changed by expression of nwk^{Δ1-428.Scer\UAS.T:Avic\GFP-EGFP} and is increased further by expression of nwk^{1-428.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^elav-C155 in the mutant background.

(Kelley et al., 2015)

Expression of nwk^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 partially rescues the synaptic overgrowth and increased bouton number phenotypes seen in nwk¹/nwk² third instar larvae.

Expression of nwk^{SH3a.Scer\UAS} under the control of Scer\GAL4^elav-C155 fails to rescue the synaptic overgrowth and increased bouton number phenotypes in nwk¹/nwk² third instar larvae.

Expression of nwk^{SH3b.Scer\UAS} under the control of Scer\GAL4^elav-C155 fails to rescue the synaptic overgrowth and increased bouton number phenotypes seen in nwk¹/nwk² third instar larvae.

Expression of nwk^{SH3ab.Scer\UAS} under the control of Scer\GAL4^elav-C155 enhances the synaptic overgrowth and increased bouton number phenotypes seen in nwk¹/nwk² third instar larvae.

Expression of nwk^ΔC.Scer\UAS under the control of Scer\GAL4^elav-C155 partially rescues the synaptic overgrowth and increased bouton number phenotypes seen in nwk¹/nwk² third instar larvae.

(Rodal et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

nwk²

(Del Signore et al., 2021, Hur et al., 2018, Stanishneva-Konovalova et al., 2016, Ukken et al., 2016, Kelley et al., 2015, Rodal et al., 2011, O'Connor-Giles et al., 2008, Rodal et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (10)

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