FB2024_03 , released June 25, 2024
Allele: Dmel\nwkUAS.cCa
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General Information
Symbol
Dmel\nwkUAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Coyle
FlyBase ID
FBal0154814
Feature type
allele
Associated gene
Dmel\nwk
Associated Insertion(s)
Carried in Construct
P{UAS-nwk.C}
Key Links
Transgenic product class
cDNA
(Coyle et al., 2004)
wild_type
(Coyle et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Coyle et al., 2004)
wild_type
(Coyle et al., 2004)
Mutagen
in vitro construct
(Coyle et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-nwk.C}
Cytology
Description

UASt regulatory sequences drive expression of a full length nwk cDNA.

(Coyle et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
nwk
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155 does not affect synaptic growth at the larval neuromuscular junction (both the average total number of boutons and the number of satellite boutons are normal in the mutant larvae).

      (O'Connor-Giles et al., 2008)

      Expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155 has no effect on synaptic growth or bouton number in third instar larvae.

      (Rodal et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of nwkScer\UAS.cCa with Atg1Scer\UAS.cSa, under the control of Scer\GAL4elav.PLu fails to suppress Atg1Scer\UAS.cSa-mediated neuromuscular junction overgrowth.

      (Shen and Ganetzky, 2009)

      Co-expression of nwkScer\UAS.cCa suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in larvae expressing two copies of tkvQ199D.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav-C155.

      Expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155 suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in homozygous Dadj1E4 larvae.

      (O'Connor-Giles et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/nwkUAS.cCa rescues nwk2

      (Coyle et al., 2004)
      Partially rescues

      nwkUAS.cCa partially rescues nwk1/nwk2

      (Rodal et al., 2008)

      nwkUAS.cCa/Scer\GAL4elav.PLu partially rescues nwk2

      (Coyle et al., 2004)
      Fails to rescue

      nwkUAS.cCa/Scer\GAL4C57 fails to rescue nwk2

      (Coyle et al., 2004)
      Comments

      Expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155 partially rescues the synaptic overgrowth and increased bouton number phenotypes seen in nwk1/nwk2 third instar larvae.

      (Rodal et al., 2008)

      The temperature sensitive paralysis phenotype of nwk2 adults is rescued by expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155; after 15 minutes at 28oC, more than 90% of the rescued adults continue to walk and climb normally. Expression of nwkScer\UAS.cCa under the control of Scer\GAL4elav.PLu partially rescues the neuromuscular junction (NMJ) phenotypes seen in nwk2 larvae; the normalised numbers of boutons and branches are fully rescued at NMJ 6/7 and NMJ 4, and the increase in NMJ length seen in nwk2 larvae is substantially rescued. The increased incidence of hyperbranched boutons per NMJ seen in nwk2 larvae is not altered in the rescued larvae. Excitatory junctional current (EJC) amplitude is fully rescued and miniature EJC amplitude is partially rescued.

      (Coyle et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      nwkScer\UAS.cCa
      nwkUAS.cCa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Coyle
      (Coyle et al., 2004)
      Secondary FlyBase IDs
        References (4)