FlyBase Allele Report: Dmel\DAAM[Ex4]

General Information

Symbol

Dmel\DAAM^Ex4

Species

D. melanogaster

Name

FlyBase ID

FBal0314794

Feature type

allele

Associated gene

Dmel\DAAM

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Gombos et al., 2015)

Mutagen

P-element activity

(Gombos et al., 2015)

Progenitor genotype

P{GawB}NP6041

(Gombos et al., 2015)

Cytology

Description

A 1293bp deletion affecting the first exon and intron of the DAAM B isoform.

(Gombos et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:1,321,084..1,322,376 [-]

Comment:

Estimated boundaries of the 1293 bp DAAM^Ex4 deletion, which was created by the imprecise excision of P{GawB}NP6041.

(Gombos et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult mushroom body alpha-lobe

(Gombos et al., 2015)

adult mushroom body alpha-lobe | adult stage

(Dollar et al., 2016)

adult mushroom body beta-lobe

(Gombos et al., 2015)

adult mushroom body beta-lobe | adult stage

(Dollar et al., 2016)

amnioserosa

(Tóth et al., 2022)

axon | adult stage

(Dollar et al., 2016)

axonal growth cone | embryonic stage

(Földi et al., 2022)

axon microtubule bundle | embryonic stage

(Földi et al., 2022)

embryonic dorsal epidermis

(Tóth et al., 2022)

growth cone filopodium | decreased number | embryonic stage, with DAAM^{I732A.UAS.Tag:FLAG}, Scer\GAL4^elav.PLu

(Földi et al., 2022)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage

(Földi et al., 2022)

lobe system of adult mushroom body

(Földi et al., 2022)

lobe system of adult mushroom body, with DAAM^{DADm.UAS.EGFP}, Scer\GAL4^ey-OK107

(Földi et al., 2022)

ommatidium | P-stage (with DAAM^Ex68)

(Gazsó-Gerhát et al., 2023)

Detailed Description

Statement

Reference

Amnioserosa cells of DAAM^Ex4 mutant embryos are bigger, present a more regular, more round shape, and exhibit a decrease in the amplitude of the cell contraction.

DAAM^Ex4 embryos exhibit dorsal closure phenotypes, such as decreased zipping speed, puckering of the dorsal epidermis and a malformed canthus; some embryos also arrests at this stage.

(Tóth et al., 2022)

DAAM^Ex4/Y males are viable and have moderate mushroom body axon growth and guidance defects.

(Dollar et al., 2016)

DAAM^Ex4 mutant flies exhibit growth and axon guidance defects in the α and β lobes of the mushroom body. No changes in Kenyon cell number or dendritic organisation are detected.

Single cell DAAM^Ex4 mutant mushroom body clones generated in a homozygous DAAM^Ex4 mutant background exhibit axon guidance and/or growth defects. In many cases the two axon branches run into the same lobe.

(Gombos et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

DAAM^Ex4, Frl⁵⁹ has abnormal neuroanatomy | P-stage phenotype, enhanceable by Frl^I773A.UAS/Scer\GAL4^54C

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has abnormal neuroanatomy | P-stage phenotype, enhanceable by Frl^I773A.UAS/Scer\GAL4^GMR.PU

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4/DAAM^Ex68 has abnormal neuroanatomy | P-stage phenotype, enhanceable by Cdc42²

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has abnormal neuroanatomy | P-stage phenotype, enhanceable by DAAM^{I732A.UAS.Tag:FLAG}/Scer\GAL4^GMR.PU

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has abnormal neuroanatomy | P-stage phenotype, enhanceable by DAAM^{I732A.UAS.Tag:FLAG}/Scer\GAL4^54C

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4 has abnormal neuroanatomy | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex83

(Dollar et al., 2016)

DAAM^Ex4 has abnormal neuroanatomy | adult stage phenotype, enhanceable by Frl^HMS00445/Scer\GAL4^ey-OK107

(Dollar et al., 2016)

DAAM^Ex4 has abnormal neuroanatomy | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex88

(Dollar et al., 2016)

DAAM^Ex4 has abnormal neuroanatomy | adult stage phenotype, enhanceable by Frl[+]/Frl^ExK62

(Dollar et al., 2016)

Suppressed by

Statement

Reference

DAAM^Ex4, Frl⁵⁹ has abnormal neuroanatomy | P-stage phenotype, suppressible | partially by Scer\GAL4^54C/Frl^UAS.cGGa

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has abnormal neuroanatomy | P-stage phenotype, suppressible by Frl^UAS.cGGa/Scer\GAL4^GMR.PU

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has abnormal neuroanatomy | P-stage phenotype, suppressible by DAAM^UAS.PB/Scer\GAL4^GMR.PU

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has abnormal neuroanatomy | P-stage phenotype, suppressible | partially by DAAM^UAS.PB/Scer\GAL4^54C

(Gazsó-Gerhát et al., 2023)

Other

Statement

Reference

DAAM^Ex4, Frl⁵⁹ has abnormal cell shape | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl^HMS00445, Scer\GAL4^GMR.PU has abnormal neuroanatomy | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl^HMS00445, Scer\GAL4^54C has abnormal neuroanatomy | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, zip² has abnormal neuroanatomy | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has visible | adult stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has lethal - all die during pharate adult stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, DAAM^RNAi.PD.UAS, Frl⁵⁹, Scer\GAL4^GMR.PU has abnormal neuroanatomy | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has abnormal neuroanatomy | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has partially lethal - majority die phenotype

(Földi et al., 2022)

DAAM^Ex4, Frl⁵⁹ has abnormal neuroanatomy | embryonic stage phenotype

(Földi et al., 2022)

DAAM^Ex4, Frl⁵⁹ has some die during P-stage phenotype

(Földi et al., 2022)

DAAM^Ex4, Frl⁵⁹ has some die during larval stage phenotype

(Földi et al., 2022)

Phenotype Manifest In

Enhanced by

Statement

Reference

DAAM^Ex4, Frl⁵⁹ has ommatidium | P-stage phenotype, enhanceable by Frl^I773A.UAS/Scer\GAL4^54C

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has ommatidium | P-stage phenotype, enhanceable by Frl^I773A.UAS/Scer\GAL4^GMR.PU

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4/DAAM^Ex68 has ommatidium | P-stage phenotype, enhanceable by Cdc42²

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has ommatidium | P-stage phenotype, enhanceable by DAAM^{I732A.UAS.Tag:FLAG}/Scer\GAL4^GMR.PU

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has ommatidium | P-stage phenotype, enhanceable by DAAM^{I732A.UAS.Tag:FLAG}/Scer\GAL4^54C

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4 has axon | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex83

(Dollar et al., 2016)

DAAM^Ex4 has adult mushroom body alpha-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^ExK62

(Dollar et al., 2016)

DAAM^Ex4 has adult mushroom body beta-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^ExK62

(Dollar et al., 2016)

DAAM^Ex4 has axon | adult stage phenotype, enhanceable by Frl^HMS00445/Scer\GAL4^ey-OK107

(Dollar et al., 2016)

DAAM^Ex4 has adult mushroom body alpha-lobe | adult stage phenotype, enhanceable by Frl^HMS00445/Scer\GAL4^ey-OK107

(Dollar et al., 2016)

DAAM^Ex4 has adult mushroom body beta-lobe | adult stage phenotype, enhanceable by Frl^HMS00445/Scer\GAL4^ey-OK107

(Dollar et al., 2016)

DAAM^Ex4 has adult mushroom body alpha-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex83

(Dollar et al., 2016)

DAAM^Ex4 has adult mushroom body beta-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex83

(Dollar et al., 2016)

DAAM^Ex4 has axon | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex88

(Dollar et al., 2016)

DAAM^Ex4 has adult mushroom body alpha-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex88

(Dollar et al., 2016)

DAAM^Ex4 has adult mushroom body beta-lobe | adult stage phenotype, enhanceable by Frl[+]/Frl^Ex88

(Dollar et al., 2016)

DAAM^Ex4 has axon | adult stage phenotype, enhanceable by Frl[+]/Frl^ExK62

(Dollar et al., 2016)

Suppressed by

Statement

Reference

DAAM^Ex4, Frl⁵⁹ has ommatidium | P-stage phenotype, suppressible by Frl^UAS.cGGa/Scer\GAL4^GMR.PU

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has ommatidium | P-stage phenotype, suppressible | partially by Scer\GAL4^54C/Frl^UAS.cGGa

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has ommatidium | P-stage phenotype, suppressible by DAAM^UAS.PB/Scer\GAL4^GMR.PU

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has ommatidium | P-stage phenotype, suppressible | partially by DAAM^UAS.PB/Scer\GAL4^54C

(Gazsó-Gerhát et al., 2023)

Other

Statement

Reference

DAAM^Ex4, Frl⁵⁹ has interommatidial cell | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has rhabdomere of eye photoreceptor cell | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl^HMS00445, Scer\GAL4^GMR.PU has ommatidium | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl^HMS00445, Scer\GAL4^54C has ommatidium | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, zip² has ommatidium | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has eye phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, DAAM^RNAi.PD.UAS, Frl⁵⁹, Scer\GAL4^GMR.PU has ommatidium | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has ommatidium | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has secondary pigment cell | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has tertiary pigment cell | P-stage phenotype

(Gazsó-Gerhát et al., 2023)

DAAM^Ex4, Frl⁵⁹ has axon microtubule bundle | embryonic stage phenotype

(Földi et al., 2022)

DAAM^Ex4, Frl⁵⁹ has axonal growth cone | embryonic stage phenotype

(Földi et al., 2022)

DAAM^Ex4, Frl⁵⁹ has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype

(Földi et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of Frl^Ex83/+, Frl^Ex88/+ or Frl^ExK62/+ enhances axon growth phenotypes seen in the mushroom body of DAAM^Ex4/Y males. Expression of Frl^HMS00445 driven by Scer\GAL4^ey-OK107 enhances axon growth phenotypes seen in the mushroom body (β and α lobes) of DAAM^Ex4/Y males.

(Dollar et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

DAAM^Ex4 is rescued by Scer\GAL4^elav.PLu/DAAM^{C.FH2R.K-A.UAS}

(Földi et al., 2022)

DAAM^Ex4 is rescued by DAAM^UASp.cMa/Scer\GAL4^elav.PLu

(Földi et al., 2022)

DAAM^Ex4 is rescued by DAAM^UASp.cMa/Scer\GAL4^ey-OK107

(Földi et al., 2022)

DAAM^Ex4 is rescued by DAAM^UASp.cMa/Scer\GAL4^elav.PU

(Gombos et al., 2015)

Partially rescued by

DAAM^Ex4 is partially rescued by DAAM^K881A.UAS/Scer\GAL4^elav.PLu

(Földi et al., 2022)

DAAM^Ex4 is partially rescued by Scer\GAL4^elav.PU/DAAM^FL-GBD2.UAS

(Gombos et al., 2015)

Not rescued by

DAAM^Ex4 is not rescued by Scer\GAL4^ey-OK107/DAAM^{C.FH2R.K-A.UAS}

(Földi et al., 2022)

DAAM^Ex4 is not rescued by DAAM^{I732A.UAS.Tag:FLAG}/Scer\GAL4^elav.PLu

(Földi et al., 2022)

Comments

Expression of DAAM^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^elav.PU rescues the α and β lobe mushroom body defects seen in DAAM^Ex4 mutant flies.

Expression of DAAM^{I732A.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PU does not rescue the α and β lobe mushroom body defects seen in DAAM^Ex4 mutant flies.

Expression of DAAM^{FL-GBD2.Scer\UAS} under the control of Scer\GAL4^elav.PU partially rescues the α and β lobe mushroom body defects seen in DAAM^Ex4 mutant flies.

(Gombos et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

DAAM^Ex4

(Gazsó-Gerhát et al., 2023, Földi et al., 2022, Tóth et al., 2022, Dollar et al., 2016)

dDAAM^Ex4

(Gombos et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (5)

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