FlyBase Allele Report: Dmel\trc[T453A.UAS.L]

General Information

Symbol

Dmel\trc^T453A.UAS.L

Species

D. melanogaster

Name

FlyBase ID

FBal0215624

Feature type

allele

Associated gene

Dmel\trc

Associated Insertion(s)

Carried in Construct

P{UAS-trc.L.T453A}

Key Links

Transgenic product class

missense_variant

(He et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(He et al., 2005)

Mutagen

in vitro construct

(He et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-trc.L.T453A}

Cytology

Description

Amino acid replacement: T453A.

(He et al., 2005)

UASt regulatory sequences drive expression of a mutated form of the long isoform of trc with the above amino acid substitution.

(He et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

trc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ap-md544

(He et al., 2005)

visible, with Scer\GAL4^ptc-559.1

(He et al., 2005)

Phenotype Manifest In

macrochaeta, with Scer\GAL4^ap-md544

(He et al., 2005)

macrochaeta, with Scer\GAL4^neur-P72

(He et al., 2005)

wing hair | increased number, with Scer\GAL4^ap-md544

(He et al., 2005, He et al., 2005)

wing hair | increased number, with Scer\GAL4^ptc-559.1

(He et al., 2005)

Detailed Description

Statement

Reference

Expression of trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ap-md544 results in a multiple wing hair phenotype. On average, 1.5-4 hairs per cell are produced but many cells still produce a single hair. Expression of trc^{T453A.Scer\UAS} in the notum, driven by either Scer\GAL4^ap-md544 or Scer\GAL4^neur-P72 results in split or branched bristles, particularly on the abdomen, and in deformed bristles that are short, fat, bent, and twisted.

Coexpression of trc^L.Scer\UAS with trc^{T453A.Scer\UAS}, under the control of Scer\GAL4^ap-md544, largely rescues the wing hair and bristle defects seen when trc^{T453A.Scer\UAS} is expressed alone.

(He et al., 2005)

Expression of trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ap-md544 or Scer\GAL4^ptc-559.1 results in a multiple wing hair phenotype.

(He et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has visible phenotype, enhanceable by mats^e03077/mats[+]

(He et al., 2005)

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has visible phenotype, enhanceable by wts[+]/wts^3-17

(He et al., 2005)

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has visible phenotype, enhanceable by Mob2^C.UAS, Scer\GAL4^ptc-559.1

(He et al., 2005)

Scer\GAL4^ap-md544, trc^T453A.UAS.L has visible phenotype, enhanceable by Mob2^C.UAS, Scer\GAL4^ap-md544

(He et al., 2005)

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has visible phenotype, enhanceable by mats^e235/mats[+]

(He et al., 2005)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has visible phenotype, non-enhanceable by mats^PBrv/mats[+]

(He et al., 2005)

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has visible phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/mats^UAS.cLa

(He et al., 2005)

Other

Statement

Reference

Rho1^N19.UAS, Scer\GAL4^ap-md544, trc^T453A.UAS.L has lethal phenotype

(He et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by fry¹/fry[+]

(He et al., 2005)

Scer\GAL4^ap-md544, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by Rac1^J11/Rac1[+]

(He et al., 2005)

Scer\GAL4^ap-md544, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by Rac1^EY05848/Rac1[+]

(He et al., 2005)

Scer\GAL4^ap-md544, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by Rho1^E3.10/Rho1[+]

(He et al., 2005)

Scer\GAL4^ap-md544, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by Cdc42[+]/Cdc42²

(He et al., 2005)

Scer\GAL4^ap-md544, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by Rala^G20V.UAS, Scer\GAL4^ap-md544

(He et al., 2005)

Scer\GAL4^ap-md544, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by bsk²/bsk[+]

(He et al., 2005)

Scer\GAL4^ap-md544, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by Graf^RNAi.UAS, Scer\GAL4^ap-md544

(He et al., 2005)

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by mats^e03077/mats[+]

(He et al., 2005)

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by wts[+]/wts^3-17

(He et al., 2005)

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by Mob2^C.UAS, Scer\GAL4^ptc-559.1

(He et al., 2005)

Scer\GAL4^ap-md544, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by Mob2^C.UAS, Scer\GAL4^ap-md544

(He et al., 2005)

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has wing hair | increased number phenotype, enhanceable by mats^e235/mats[+]

(He et al., 2005)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has wing hair | increased number phenotype, non-enhanceable by mats^PBrv/mats[+]

(He et al., 2005)

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, trc^T453A.UAS.L has wing hair | increased number phenotype, suppressible by Rala^S25N.UAS, Scer\GAL4^ap-md544

(He et al., 2005)

Scer\GAL4^ap-md544, trc^T453A.UAS.L has wing hair | increased number phenotype, suppressible | partially by vimar^k16722

(He et al., 2005)

Scer\GAL4^ptc-559.1, trc^T453A.UAS.L has wing hair | increased number phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/mats^UAS.cLa

(He et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

A fry¹/+ background enhances the multiple wing hair phenotype of flies that express trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ap-md544.

The following genetic backgrounds enhance the splitting wing hair phenotype of flies that express trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ap-md544 : Rac1^J11/+, Rac1^EY05848/+, Rho1^E3.10/+, Cdc42²/+ and bsk²/+.

Coexpression of Rho1^N19.Scer\UAS and trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ap-md544 induces synthetic lethality.

Coexpression of the dominant negative Rala^{S25N.Scer\UAS} with trc^{T453A.Scer\UAS}, under the control of Scer\GAL4^ap-md544, leads to a complete suppression of the trc^{T453A.Scer\UAS} phenotype. In contrast, coexpression of the constitutively active Rala^{G20V.Scer\UAS} transgene with trc^{T453A.Scer\UAS}, under the control of Scer\GAL4^ap-md544, results in a dramatic enhancement of the multiple wing hair phenotype.

Coexpression of Graf^{dsRNA.Scer\UAS} greatly enhances the Scer\GAL4^ap-md544>trc^{T453A.Scer\UAS} multiple wing hair phenotype.

A vimar^k16722 background partially suppresses the multiple wing hair phenotype seen when trc^{T453A.Scer\UAS} is expressed under the control of Scer\GAL4^ap-md544.

(He et al., 2005)

The multiple wing hair phenotype that is caused by expression of trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ap-md544 is enhanced by a deficiency for one of mats, Mob2, Mob3 or Mob4.

The multiple wing hair phenotype that is caused by expression of trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 is dominantly enhanced by wts^3-17, mats^e235 or mats^e03077, but not by mats^PBrv.

The multiple wing hair phenotype that is caused by expression of trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 is partially suppressed by co-expression of mats^Scer\UAS.cLa.

The multiple wing hair phenotype that is caused by expression of trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 or Scer\GAL4^ap-md544 is enhanced by co-expression of Mob2^C.Scer\UAS.

(He et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^Act5C.PI/trc^T453A.UAS.L fails to rescue trc^S066917a

(He et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

32083

w^*; P{UAS-trc.L.T453A}2

32088

w^*; P{UAS-trc.L.T453A}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

trc^{T453A.Scer\UAS.L}

trc^{T453A.Scer\UAS}

trc^T453A.UAS.L

trc^T453A

(He et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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