FB2024_03 , released June 25, 2024
Allele: Dmel\RalaG20V.UAS
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General Information
Symbol
Dmel\RalaG20V.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0101576
Feature type
allele
Associated gene
Dmel\Rala
Associated Insertion(s)
Carried in Construct
P{UAS-Rala.G20V}
Also Known As
DRalG20V, UAS-RalAG20V
Key Links
Transgenic product class
missense_variant
(Sawamoto et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Sawamoto et al., 1999)
Mutagen
in vitro construct
(Sawamoto et al., 1999)
Progenitor genotype
Carried in construct
P{UAS-Rala.G20V}
Cytology
Description

A constitutively active form of Rala (carries the amino acid replacement )G20V is expressed under the control of UASt regulatory sequences.

(Sawamoto et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rala
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4en-e16E-mediated expression of RalaG20V.Scer\UAS causes loss of crossveins.

      (Blanke and Jackle, 2006)

      Expression of RalaG20V.Scer\UAS under the control of Scer\GAL4amn-c651 has no effect on body size.

      (Caldwell et al., 2005)

      Expression of RalaG20V.Scer\UAS under the control of Scer\GAL4Act5C.PP in wing disc cells results in a decrease in cell size without affecting cell cycle phasing.

      (Prober and Edgar, 2002)

      Embryonic expression of RalaG20V.Scer\UAS under the control of Scer\GAL4unspecified results in lethality. In some cases, the embryonic cuticle has defects on the dorsal surface, indicating that dorsal closure is defective. Expression of RalaG20V.Scer\UAS in the leading edge under the control of Scer\GAL4pnr-MD237 or Scer\GAL4LE results in large holes in the anterior or dorsal epidermis of the embryo. Some embryos show a severe dorsal-open phenotype.

      (Sawamoto et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Coexpression of the dominant negative RalaS25N.Scer\UAS with trcT453A.Scer\UAS, under the control of Scer\GAL4ap-md544, leads to a complete suppression of the trcT453A.Scer\UAS phenotype. In contrast, coexpression of the constitutively active RalaG20V.Scer\UAS transgene with trcT453A.Scer\UAS, under the control of Scer\GAL4ap-md544, results in a dramatic enhancement of the multiple wing hair phenotype.

      (He et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4Rala-PG69/RalaG20V.UAS rescues RalaPG69

      (Ghiglione et al., 2008)
      Comments

      Expression of RalaG20V.Scer\UAS under the control of Scer\GAL4Rala-PG69 in a RalaPG69 mutant background rescues lethality, and border cell migration.

      (Ghiglione et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in plasmid "pUAST-DRalG20V", transfected into S2 cells and used in phosphorylation assays.

      (Sawamoto et al., 1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      DRalG20V
      (Sawamoto et al., 1999)
      DralG20V
      (He et al., 2005)
      RalV20
      (Caldwell et al., 2005)
      RalaG20V.Scer\UAS
      RalaG20V.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (9)