FlyBase Allele Report: Dmel\Rala[PG69]

General Information

Symbol

Dmel\Rala^PG69

Species

D. melanogaster

Name

FlyBase ID

FBal0130802

Feature type

allele

Associated gene

Dmel\Rala

Associated Insertion(s)

P{GawB}Rala^PG69

Carried in Construct

Also Known As

PG69

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Bourbon et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{GawB}Rala^PG69

Cytology

Description

Insertion in the 5' untranslated region.

(Ghiglione et al., 2008)

Insertion in the first exon.

(Balakireva et al., 2006)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:3,719,363..3,719,635

(Ghiglione et al., 2008, Balakireva et al., 2006, Bourbon et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | somatic clone

(Ghiglione et al., 2008)

lethal

(Balakireva et al., 2006)

lethal | recessive

(Bourbon et al., 2002)

Phenotype Manifest In

border follicle cell | somatic clone

(Ghiglione et al., 2008)

Detailed Description

Statement

Reference

Mosaic stage 10 Rala^PG69 oocytes show a complete lack of border cell migration.

Mosaic Rala^PG69 clones where the outer border cells are mutant but the two polar cells are wildtype show normal formation of the border cell cluster, and normal border cell migration.

When one of two polar cells is mutant for Rala^PG69, a reduced number of border cells are seen in the cluster, and it does not migrate.

When both of the polar cells are mutant for Rala^PG69, almost no outer border cells are recruited.

Some polar cells, but not other mutant follicle cells, show increased cell death when homozygous mutant for Rala^PG69.

(Ghiglione et al., 2008)

Adult hemizygous males die during embryogenesis from stage 13 through the first-instar larval stage.

(Balakireva et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rala^PG69 has lethal phenotype, suppressible | partially by Scer\GAL4^Rala-PG69/Jra^DN.UAS

(Balakireva et al., 2006)

Suppressor of

Statement

Reference

Rala[+]/Rala^PG69 is a suppressor of visible phenotype of lqf^FDD9

(Cho and Fischer, 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Rala[+]/Rala^PG69 is a suppressor of ommatidium phenotype of lqf^FDD9

(Cho and Fischer, 2011)

Rala[+]/Rala^PG69 is a suppressor of eye photoreceptor cell phenotype of lqf^FDD9

(Cho and Fischer, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Rala^PG69 dominantly suppresses the rough eye phenotype of lqf^FDD9 homozygotes, with a greater proportion of ommatidia containing the normal number of photoreceptor cells in the double mutant flies.

(Cho and Fischer, 2011)

Expression of Jra^DN.Scer\UAS under the control of Scer\GAL4^Rala-PG69 rescues Rala^PG69 hemizygous males to the pharate adult stage.

(Balakireva et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rala^PG69 is rescued by Scer\GAL4^Rala-PG69/Rala^UAS.cSa

(Ghiglione et al., 2008)

Rala^PG69 is rescued by Scer\GAL4^Rala-PG69/Rala^G20V.UAS

(Ghiglione et al., 2008)

Rala^PG69 is rescued by Rala^N.UAS.GFP/Scer\GAL4^Rala-PG69

(Ghiglione et al., 2008)

Rala^PG69 is rescued by Scer\GAL4^da.G32/Rala^UAS.cMa

(Balakireva et al., 2006)

Not rescued by

Rala^PG69 is not rescued by Rala^S25N.UAS/Scer\GAL4^Rala-PG69

(Ghiglione et al., 2008)

Rala^PG69 is not rescued by Scer\GAL4^Rala-PG69/Rala^{ΔCTLL.N.UAS.GFP}

(Ghiglione et al., 2008)

Rala^PG69 is not rescued by Rala^C.UAS.GFP/Scer\GAL4^Rala-PG69

(Ghiglione et al., 2008)

Comments

Expression of Rala^Scer\UAS.cSa under the control of Scer\GAL4^Rala-PG69 in a Rala^PG69 mutant background rescues lethality, and border cell migration.

Expression of Rala^{G20V.Scer\UAS} under the control of Scer\GAL4^Rala-PG69 in a Rala^PG69 mutant background rescues lethality, and border cell migration.

Expression of Rala^{S25N.Scer\UAS} under the control of Scer\GAL4^Rala-PG69 in a Rala^PG69 mutant background does not rescue lethality and border cell migration.

Expression of Rala^{N.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Rala-PG69 in a Rala^PG69 mutant background rescues border cell migration.

Expression of Rala^{ΔCTLL.N.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Rala-PG69 in a Rala^PG69 mutant background does not rescue border cell migration.

Expression of Rala^{C.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Rala-PG69 in a Rala^PG69 mutant background does not rescue border cell migration.

(Ghiglione et al., 2008)

Expression of Rala^Scer\UAS.cMa under the control of Scer\GAL4^da.G32 restores viability in Rala^PG69 hemizygous males.

(Balakireva et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Precise excision of the insertion reverts the lethal phenotype.

(Balakireva et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

PG69

(Ghiglione et al., 2008, Ghiglione et al., 2008)

Ral^PG69

(Cho and Fischer, 2011)

RalA^PG69

(Ghiglione et al., 2008, Ghiglione et al., 2008)

Rala^PG69

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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