FlyBase Allele Report: Dmel\Cip4[1]

General Information

Symbol

Dmel\Cip4¹

Species

D. melanogaster

Name

FlyBase ID

FBal0260999

Feature type

allele

Associated gene

Dmel\Cip4

Associated Insertion(s)

Carried in Construct

Also Known As

dcip4¹

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Nahm et al., 2010)

Progenitor genotype

Cip4^EP3507

(Nahm et al., 2010)

Cytology

Description

Imprecise excision of P{EP}Cip4^EP3507 generates a deletion that removes part of the Cip4¹ first exon including the initiation codon, the first intron, and the second exon of the Cip4 gene.

(Nahm et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Nahm et al., 2010)

abnormal neuroanatomy (with Df(3L)ED4342)

(Nahm et al., 2010)

abnormal neuroanatomy | third instar larval stage (with Df(3L)ED4342)

(Nahm et al., 2010)

partially lethal - majority die

(Nahm et al., 2010)

Phenotype Manifest In

neuromuscular junction

(Nahm et al., 2010)

neuromuscular junction (with Df(3L)ED4342)

(Nahm et al., 2010)

NMJ bouton

(Nahm et al., 2010)

NMJ bouton (with Df(3L)ED4342)

(Nahm et al., 2010)

NMJ bouton | third instar larval stage (with Df(3L)ED4342)

(Nahm et al., 2010)

Detailed Description

Statement

Reference

Third instar Cip4¹/Df(3L)ED4342 larvae show an increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction compared to wild type.

(Nahm et al., 2010)

Cip4¹ homozygotes and Cip4¹/Df(3L)ED4342 trans-heterozygotes are semi-lethal at the pupal stage with an incidence of lethality of ~17%.

Cip4¹ mutant larvae exhibit an increase in bouton number, satellite bouton number and neuromuscular junction length.

Compared with wild-type controls, Cip4¹/Df(3L)ED4342 trans-heterozygous mutant larvae exhibit an increase in bouton number normalized to muscle surface area of approximately 32%. In addition, satellite bouton number and neuromuscular junction length normalized to muscle surface area are increased by 67% and 24%, respectively.

Cip4¹ heterozygous neuromuscular junctions do not exhibit any morphological changes.

(Nahm et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cip4¹ has abnormal neuroanatomy phenotype, enhanceable by Cdc42[+]/WASp[+]/WASp¹/Cdc42²

(Nahm et al., 2010)

Cip4¹ has abnormal neuroanatomy phenotype, enhanceable by Cdc42[+]/Cdc42²

(Nahm et al., 2010)

Cip4¹ has abnormal neuroanatomy phenotype, enhanceable by WASp[+]/WASp¹

(Nahm et al., 2010)

Cip4¹ has abnormal neuroanatomy phenotype, enhanceable by Arp3^EP3640/Arp66B[+]

(Nahm et al., 2010)

NOT suppressed by

Statement

Reference

Df(3L)ED4342/Cip4¹ has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

Enhancer of

Statement

Reference

Cdc42[+], Cip4¹, Cip4[+], Cdc42² is an enhancer of abnormal neuroanatomy phenotype of WASp¹

(Nahm et al., 2010)

Cip4¹/Cip4[+] is an enhancer of abnormal neuroanatomy phenotype of Cdc42²

(Nahm et al., 2010)

Cip4¹, WASp[+], WASp¹, Cip4[+] is an enhancer of abnormal neuroanatomy phenotype of Cdc42²

(Nahm et al., 2010)

Cip4¹/Cip4[+] is an enhancer of abnormal neuroanatomy phenotype of Arp3^EP3640

(Nahm et al., 2010)

Suppressor of

Statement

Reference

Cip4¹/Cip4[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cip4¹ has NMJ bouton phenotype, enhanceable by Cdc42[+]/Cdc42²

(Nahm et al., 2010)

Cip4¹ has neuromuscular junction phenotype, enhanceable by Cdc42[+]/Cdc42²

(Nahm et al., 2010)

Cip4¹ has neuromuscular junction phenotype, enhanceable by Cdc42[+]/WASp[+]/WASp¹/Cdc42²

(Nahm et al., 2010)

Cip4¹ has NMJ bouton phenotype, enhanceable by WASp[+]/WASp¹

(Nahm et al., 2010)

Cip4¹ has neuromuscular junction phenotype, enhanceable by WASp[+]/WASp¹

(Nahm et al., 2010)

Cip4¹ has NMJ bouton phenotype, enhanceable by Arp3^EP3640/Arp66B[+]

(Nahm et al., 2010)

Cip4¹ has neuromuscular junction phenotype, enhanceable by Arp3^EP3640/Arp66B[+]

(Nahm et al., 2010)

Cip4¹ has NMJ bouton phenotype, enhanceable by Cdc42[+]/WASp[+]/WASp¹/Cdc42²

(Nahm et al., 2010)

NOT suppressed by

Statement

Reference

Df(3L)ED4342/Cip4¹ has NMJ bouton | third instar larval stage phenotype, non-suppressible by RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

Enhancer of

Statement

Reference

Cip4¹/Cip4[+] is an enhancer of NMJ bouton phenotype of WASp¹

(Nahm et al., 2010)

Cip4¹/Cip4[+] is an enhancer of neuromuscular junction phenotype of WASp¹

(Nahm et al., 2010)

Cdc42[+], Cip4¹, Cip4[+], Cdc42² is an enhancer of NMJ bouton phenotype of WASp¹

(Nahm et al., 2010)

Cdc42[+], Cip4¹, Cip4[+], Cdc42² is an enhancer of neuromuscular junction phenotype of WASp¹

(Nahm et al., 2010)

Cip4¹/Cip4[+] is an enhancer of NMJ bouton phenotype of Cdc42²

(Nahm et al., 2010)

Cip4¹/Cip4[+] is an enhancer of neuromuscular junction phenotype of Cdc42²

(Nahm et al., 2010)

Cip4¹, WASp[+], WASp¹, Cip4[+] is an enhancer of NMJ bouton phenotype of Cdc42²

(Nahm et al., 2010)

Cip4¹, WASp[+], WASp¹, Cip4[+] is an enhancer of neuromuscular junction phenotype of Cdc42²

(Nahm et al., 2010)

Cip4¹/Cip4[+] is an enhancer of NMJ bouton phenotype of Arp3^EP3640

(Nahm et al., 2010)

Cip4¹/Cip4[+] is an enhancer of neuromuscular junction phenotype of Arp3^EP3640

(Nahm et al., 2010)

Suppressor of

Statement

Reference

Cip4¹/Cip4[+] is a suppressor of NMJ bouton | third instar larval stage phenotype of RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction seen in third instar Cip4¹/Df(3L)ED4342 larvae is not altered if they are also mutant for RhoGAP92B¹/RhoGAP92B².

The decrease in bouton number/muscle area and satellite bouton number at the neuromuscular junction which is seen in third instar RhoGAP92B¹/RhoGAP92B² larvae is significantly suppressed by Cip4¹/+.

(Nahm et al., 2010)

Cdc42²; Cip4¹ double heterozygotes exhibit an increase in total bouton number, satellite bouton formation and neuromuscular junction length.

WASp¹/Cip4¹ double heterozygotes exhibit an increase in total bouton number, satellite bouton formation and neuromuscular junction length.

Arp66B^EP3640/Cip4¹ double heterozygotes exhibit an increase in total bouton number, satellite bouton formation and neuromuscular junction length.

Cdc42²/+; Cip4¹/+; WASp¹/+ trans-heterozygous larvae exhibit an increase in total bouton number, neuromuscular junction length and in particular satellite bouton formation.

(Nahm et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cip4¹ is rescued by Scer\GAL4^da.G32/Cip4^UAS.N.Tag:HA

(Nahm et al., 2010)

Df(3L)ED4342/Cip4¹ is rescued by Scer\GAL4^C57/Cip4^UAS.N.Tag:HA

(Nahm et al., 2010)

Df(3L)ED4342/Cip4¹ is rescued by Cip4^{L145D.UAS.Tag:HA}/Scer\GAL4^C57

(Nahm et al., 2010)

Not rescued by

Df(3L)ED4342/Cip4¹ is not rescued by Scer\GAL4^elav-C155/Cip4^UAS.N.Tag:HA

(Nahm et al., 2010)

Df(3L)ED4342/Cip4¹ is not rescued by Cip4^{L436S.UAS.Tag:HA}/Scer\GAL4^C57

(Nahm et al., 2010)

Df(3L)ED4342/Cip4¹ is not rescued by Scer\GAL4^C57/Cip4^{W603K.UAS.Tag:HA}

(Nahm et al., 2010)

Comments

Ubiquitous expression of Cip4^{Scer\UAS.N.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 leads to a nearly complete rescue of Cip4¹ lethality.

Expression of Cip4^{Scer\UAS.N.T:Ivir\HA1} in Cip4¹/Df(3L)ED4342 trans-heterozygotes under the control of the muscle-specific Scer\GAL4^C57 fully rescues the synaptic overgrowth phenotype.

Expression of Cip4^{Scer\UAS.N.T:Ivir\HA1} in Cip4¹/Df(3L)ED4342 trans-heterozygotes under the control of the neuron-specific Scer\GAL4^elav-C155 fails to rescue the synaptic overgrowth phenotype.

Post-synaptic expression of Cip4^{L145D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^C57 rescues the synaptic overgrowth phenotype found in Cip4¹/Df(3L)ED4342 trans-heterozygotes.

Post-synaptic expression of Cip4^{L436S.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^C57 fails to rescue the synaptic overgrowth phenotype found in Cip4¹/Df(3L)ED4342 trans-heterozygotes.

Post-synaptic expression of Cip4^{W603K.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^C57 fails to rescue the synaptic overgrowth phenotype found in Cip4¹/Df(3L)ED4342 trans-heterozygotes.

(Nahm et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cip4¹

dcip4¹

(Nahm et al., 2010, Nahm et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (2)

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