FB2024_03 , released June 25, 2024
Allele: Dmel\RhoGAP92B2
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General Information
Symbol
Dmel\RhoGAP92B2
Species
D. melanogaster
Name
FlyBase ID
FBal0267120
Feature type
allele
Associated gene
Dmel\RhoGAP92B
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Nahm et al., 2010)
Progenitor genotype
P{EP}RhoGAP92BG6428
(Nahm et al., 2010)
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in a deletion from -129 to 6550 relative to the predicted translation start site.

(Nahm et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:19,905,182..19,911,860 [+]
Comment:

Deletion (nucleotides -129 to 6550 relative to the predicted RhoGAP92B translation start site) resulting from the imprecise excision of P{EP}RhoGAP92BG4993.

(Nahm et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygotes and RhoGAP92B1/RhoGAP92B2 animals have no obvious defects in pathfinding of motor axons.

      Third instar RhoGAP92B1/RhoGAP92B2 larvae show a mild increase in muscle area. They show a 43% reduction in bouton number/muscle area and a 37% reduction in neuromuscular junction (NMJ) length/muscle area compared to wild type. Mean bouton size is increased by 69% compared to controls. Muscle area and NMJ length are normal in first instar RhoGAP92B1/RhoGAP92B2 larvae.

      Ultrastructurally, type I boutons show a number of defects in RhoGAP92B1/RhoGAP92B2 third instar larvae. The cross-sectional bouton area is increased and the number of active zones/bouton area is reduced, while the number of T bars/bouton area is normal. Subsynaptic reticulum (SSR) thickness/bouton area is normal, SSR density is reduced and total non-tubular sarcoplasmic area/SSR area is increased.

      The amplitude of the excitatory junctional current (EJC) is reduced in RhoGAP92B1/RhoGAP92B2 third instar larvae compared to wild type. The amplitude of the miniature EJC is normal, but there is a small but significant increase in mEJC frequency.

      (Nahm et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction seen in third instar Cip41/Df(3L)ED4342 larvae is not altered if they are also mutant for RhoGAP92B1/RhoGAP92B2.

      The decrease in bouton number/muscle area and satellite bouton number at the neuromuscular junction which is seen in third instar RhoGAP92B1/RhoGAP92B2 larvae is significantly suppressed if the larvae are also mutant for Cip41/+, WASp1/+ or Dadj1E4/+.

      The increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction seen in third instar WASp1/Df(3R)3450 larvae is partially suppressed by RhoGAP92B1/RhoGAP92B2.

      RhoGAP92B1/+ gbb1/+ and RhoGAP92B1/+ witA12/+ double heterozygous third instar larvae show a significant decrease in bouton number/muscle area and satellite bouton number at the neuromuscular junction compared to wild type.

      The increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction which is seen in homozygous Dadj1E4 third instar larvae is not altered if they are also mutant for RhoGAP92B1/RhoGAP92B2.

      Expression of gbbScer\UAS.T:Avic\GFP under the control of Scer\GAL4C57 partially suppresses the reduction in bouton number/muscle area and satellite bouton number at the neuromuscular junction seen in third instar RhoGAP92B1/RhoGAP92B2 larvae.

      (Nahm et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      RhoGAP92B1/RhoGAP92B2 is rescued by RhoGAP92BUAS.cNa/Scer\GAL4C57

      (Nahm et al., 2010)
      Partially rescued by

      RhoGAP92B1/RhoGAP92B2 is partially rescued by RhoGAP92BR287A.UAS/Scer\GAL4C57

      (Nahm et al., 2010)

      RhoGAP92B1/RhoGAP92B2 is partially rescued by RhoGAP92BΔBAR.UAS.GFP/Scer\GAL4C57

      (Nahm et al., 2010)
      Not rescued by

      RhoGAP92B1/RhoGAP92B2 is not rescued by Scer\GAL4elav-C155/RhoGAP92BUAS.cNa

      (Nahm et al., 2010)
      Comments

      The muscle and neuromuscular junction growth defects seen in third instar RhoGAP92B1/RhoGAP92B2 larvae are rescued by expression of RhoGAP92BScer\UAS.cNa in the muscle under the control of Scer\GAL4C57 but are not rescued by neuronal expression of RhoGAP92BScer\UAS.cNa under the control of Scer\GAL4elav-C155.

      The electrophysiological defects seen in third instar RhoGAP92B1/RhoGAP92B2 larvae are not rescued by expression of RhoGAP92BScer\UAS.cNa under the control of Scer\GAL4elav-C155 but are completely rescued by expression of RhoGAP92BScer\UAS.cNa in the muscle under the control of Scer\GAL4C57.

      Expression of RhoGAP92BR287A.Scer\UAS in the muscle under the control of Scer\GAL4C57 rescues the increase in muscle size seen in third instar RhoGAP92B1/RhoGAP92B2 larvae. The reduced neuromuscular junction length/muscle area, increased bouton size, reduced bouton number/muscle area, increased bouton area and reduced number of active zones/bouton area are not rescued, while the subsynaptic reticulum defects are partly or completely rescued.

      The electrophysiological defects seen in third instar RhoGAP92B1/RhoGAP92B2 larvae are not rescued by expression of RhoGAP92BR287A.Scer\UAS under the control of Scer\GAL4C57.

      Expression of RhoGAP92BΔBAR.Scer\UAS.T:Avic\GFP in the muscle under the control of Scer\GAL4C57 does not rescue the increase in muscle size seen in third instar RhoGAP92B1/RhoGAP92B2 larvae. The increased bouton size, increased bouton area and reduced number of active zones/bouton area are rescued, but the subsynaptic reticulum defects are not rescued.

      The electrophysiological defects seen in third instar RhoGAP92B1/RhoGAP92B2 larvae are rescued by expression of RhoGAP92BΔBAR.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4C57.

      (Nahm et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      RhoGAP92B2
      drich2
      (Nahm et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)