FlyBase Allele Report: Dmel\Ank2[f00518]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Dmel\Ank2^f00518

General Information

Symbol

Dmel\Ank2^f00518

Species

D. melanogaster

Name

FlyBase ID

FBal0188204

Feature type

allele

Associated gene

Associated Insertion(s)

PBac{WH}Ank2^f00518

Carried in Construct

Also Known As

ank2⁵¹⁸, ank2^null

Key Links

Genomic Maps

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{WH}Ank2^f00518

Cytology

Description

Allele components

Component

Use(s)

Inserted element

misexpression element

Regulatory region(s)

binary expression system - regulatory region

Also carries

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:7,707,163..7,707,163 [+]

(Chen et al., 2023, Bulat et al., 2014, Pielage et al., 2008, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of epilepsy

CEA with Ank2^MB04517

(Chen et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Pielage et al., 2008)

abnormal neuroanatomy, with Df(3L)RM5-2

(Pielage et al., 2008)

abnormal neuroanatomy (with Ank2^f02001)

(Pielage et al., 2008)

abnormal neuroanatomy | larval stage

(Stephan et al., 2015)

abnormal neuroanatomy | larval stage, with Ank2^XL-5xR

(Stephan et al., 2015)

abnormal neuroanatomy | larval stage, with Ank2^XL-20xR

(Stephan et al., 2015)

abnormal neuroanatomy | larval stage, with Ank2^XL-Δall

(Stephan et al., 2015)

abnormal neuroanatomy | larval stage, with Ank2^XL-ΔC

(Stephan et al., 2015)

abnormal neuroanatomy | larval stage, with Ank2^XL-ΔR

(Stephan et al., 2015)

abnormal neurophysiology | second instar larval stage, with Df(3L)RM5-2

(Pielage et al., 2008)

(Stephan et al., 2015)

lethal (with Ank2^f02001)

(Pielage et al., 2008)

lethal - all die before end of larval stage, with Df(3L)RM5-2

(Pielage et al., 2008)

lethal - all die before end of larval stage | recessive

(Pielage et al., 2008)

some die during larval stage, with Df(3L)RM5-2

(Pielage et al., 2008)

some die during larval stage | recessive

(Pielage et al., 2008)

Phenotype Manifest In

axon | larval stage, with Ank2^XL-5xR

(Stephan et al., 2015)

axon | larval stage, with Ank2^XL-20xR

(Stephan et al., 2015)

axon | larval stage, with Ank2^XL-Δall

(Stephan et al., 2015)

axon | larval stage, with Ank2^XL-ΔC

(Stephan et al., 2015)

axon | larval stage, with Ank2^XL-ΔR

(Stephan et al., 2015)

embryonic/larval neuromuscular junction | larval stage

(Migh et al., 2018, Stephan et al., 2015)

embryonic/larval neuromuscular junction | larval stage, with Ank2^XL-5xR

(Stephan et al., 2015)

embryonic/larval neuromuscular junction | larval stage, with Ank2^XL-20xR

(Stephan et al., 2015)

embryonic/larval neuromuscular junction | larval stage, with Ank2^XL-Δall

(Stephan et al., 2015)

embryonic/larval neuromuscular junction | larval stage, with Ank2^XL-ΔC

(Stephan et al., 2015)

embryonic/larval neuromuscular junction | larval stage, with Ank2^XL-ΔR

(Stephan et al., 2015)

larval LL1 motor neuron | larval stage

(Migh et al., 2018)

microtubule | larval stage

(Migh et al., 2018, Stephan et al., 2015)

microtubule | larval stage, with Ank2^XL-5xR

(Stephan et al., 2015)

microtubule | larval stage, with Ank2^XL-20xR

(Stephan et al., 2015)

microtubule | larval stage, with Ank2^XL-Δall

(Stephan et al., 2015)

microtubule | larval stage, with Ank2^XL-ΔC

(Stephan et al., 2015)

microtubule | larval stage, with Ank2^XL-ΔR

(Stephan et al., 2015)

neuromuscular junction

(Bulat et al., 2014, Pielage et al., 2008)

neuromuscular junction, with Df(3L)RM5-2

(Pielage et al., 2008)

neuromuscular junction (with Ank2^f02001)

(Pielage et al., 2008)

NMJ bouton, with Df(3L)RM5-2

(Pielage et al., 2008)

NMJ bouton | larval stage

(Migh et al., 2018)

(Bulat et al., 2014)

synapse | larval stage

(Stephan et al., 2015)

synapse | larval stage, with Ank2^XL-Δall

(Stephan et al., 2015)

synapse | larval stage, with Ank2^XL-ΔC

(Stephan et al., 2015)

type Ib motor neuron | larval stage, with Ank2^XL-5xR

(Stephan et al., 2015)

type Ib motor neuron | larval stage, with Ank2^XL-20xR

(Stephan et al., 2015)

type Ib motor neuron | larval stage, with Ank2^XL-Δall

(Stephan et al., 2015)

type Ib motor neuron | larval stage, with Ank2^XL-ΔC

(Stephan et al., 2015)

type Ib motor neuron | larval stage, with Ank2^XL-ΔR

(Stephan et al., 2015)

Detailed Description

Statement

Reference

Larval muscle 4 neuromuscular junctions in Ank2^XLΔ homozygotes display a significant decrease in the number of synaptic boutons, in parallel with bouton fusion and pronounced microtubule accumulation in the nerve terminal, as compared to controls; heterozygotes, however, do not show significant changes in synaptic bouton number, as compared to controls.

(Migh et al., 2018)

Ank2^f00518/Ank2^f00518 mutant larvae exhibit lethality, and neuromuscular junctions exhibit defects in synaptic morphology and stability, and defects in microtubule organization.

Expression of Ank2^XL-Δall or Ank2^XL-ΔC in a Ank2^f00518/Ank2^f00518 background in larvae results in neuromuscular junctions exhibiting defective microtubule organization and synapse morphology, and the type 1b axons innervating NMJs exhibit aberrant axonal microtubule accumulations, as compared to controls.

Expression of Ank2^XL-5xR, Ank2^XL-ΔR, or Ank2^XL-20xR, in a Ank2^f00518/Ank2^f00518 background in larvae results in type 1b axons innervating NMJs exhibit aberrant axonal microtubule accumulations, as compared to controls.

(Stephan et al., 2015)

Ank2^f00518 homozygous mutants exhibit a synaptic retraction phenotype.

(Bulat et al., 2014)

Homozygous and Ank2^f00518/Df(3L)RM5-2 animals show severe synaptic retractions at 29[o]C of neuromuscular junctions.

Ank2^f02001/Ank2^f00518 animals die as third instar larvae/early pupae, and they show severe synaptic retractions at the neuromuscular junctions.

Ank2^f00518/Df(3L)RM5-2 second instar larvae show defects in synaptic transmission and motoneuron excitability at the NMJ. Defects in in synapse morphology are also seen at the NMJ, with loss of the narrow interbouton regions and loss of discrete, evenly spaced boutons. The presynaptic microtubule cytoskeleton is severely disorganised.

(Pielage et al., 2008)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Ank2^f00518 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by futsch^K68/futsch^K68

(Stephan et al., 2015)

Suppressor of

Statement

Reference

Ank2^XL-ΔR, Ank2^f00518/Ank2^f00518 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Ank2^XL-5xR, Ank2^f00518/Ank2^f00518 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Ank2^XL-20xR, Ank2^f00518/Ank2^f00518 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

NOT Suppressor of

Statement

Reference

Ank2^XL-Δall, Ank2^f00518/Ank2^f00518 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Ank2^XL-ΔC, Ank2^f00518/Ank2^f00518 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ank2^f00518 has embryonic/larval neuromuscular junction | larval stage phenotype, enhanceable by DAAM^Ex68/DAAM^Ex68

(Migh et al., 2018)

Ank2^f00518 has NMJ bouton | larval stage phenotype, enhanceable by DAAM^Ex68/DAAM^Ex68

(Migh et al., 2018)

Suppressed by

Statement

Reference

Ank2^f00518 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by DAAM^Ex68/DAAM^Ex68

(Migh et al., 2018)

Ank2^f00518 has microtubule | larval stage phenotype, suppressible | partially by DAAM^Ex68/DAAM^Ex68

(Migh et al., 2018)

NOT suppressed by

Statement

Reference

Ank2^f00518 has neuromuscular junction | larval stage phenotype, non-suppressible by futsch^K68/futsch^K68

(Stephan et al., 2015)

Ank2^f00518 has synapse | larval stage phenotype, non-suppressible by futsch^K68/futsch^K68

(Stephan et al., 2015)

Enhancer of

Statement

Reference

Ank2^f00518/Ank2^f00518 is an enhancer of NMJ bouton | larval stage phenotype of DAAM^Ex68

(Migh et al., 2018)

Suppressor of

Statement

Reference

Ank2^f00518/Ank2^f00518 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of DAAM^Ex68

(Migh et al., 2018)

Ank2^XL-ΔR, Ank2^f00518/Ank2^f00518 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Ank2^XL-ΔR, Ank2^f00518/Ank2^f00518 is a suppressor | partially of microtubule | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Ank2^XL-5xR, Ank2^f00518/Ank2^f00518 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Ank2^XL-5xR, Ank2^f00518/Ank2^f00518 is a suppressor | partially of microtubule | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Ank2^XL-20xR, Ank2^f00518/Ank2^f00518 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Ank2^XL-20xR, Ank2^f00518/Ank2^f00518 is a suppressor | partially of microtubule | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

NOT Suppressor of

Statement

Reference

Ank2[+]/Ank2^f00518 is a non-suppressor of NMJ bouton | larval stage phenotype of DAAM^Ex68

(Migh et al., 2018)

Ank2[+]/Ank2^f00518 is a non-suppressor of larval LL1 motor neuron | larval stage phenotype of DAAM^Ex68

(Migh et al., 2018)

Ank2[+]/Ank2^f00518 is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of DAAM^Ex68

(Migh et al., 2018)

Ank2^XL-Δall, Ank2^f00518/Ank2^f00518 is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Ank2^XL-Δall, Ank2^f00518/Ank2^f00518 is a non-suppressor of microtubule | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Ank2^XL-ΔC, Ank2^f00518/Ank2^f00518 is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Ank2^XL-ΔC, Ank2^f00518/Ank2^f00518 is a non-suppressor of microtubule | larval stage phenotype of futsch^K68

(Stephan et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

futsch^K68/futsch^K68 fails to suppress the defective synaptic stability of Ank2^f00518/Ank2^f00518 mutant larval neuromuscular junctions.

(Stephan et al., 2015)

Presynaptic expression of CkIIα^{Scer\UAS.N.T:Ivir\HA1} in Ank2^f00518 homozygous mutants fails to alleviate the synaptic retraction phenotype.

Presynaptic expression of CkIIα^{K66M.Scer\UAS.N.T:Ivir\HA1} in Ank2^f00518 homozygous mutants does not affect the synaptic retraction phenotype.

(Bulat et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

(Pielage et al., 2008)

Rescued by

Ank2^f00518 is rescued by Ank2^+t82

(Stephan et al., 2015)

Partially rescued by

Ank2^f00518 is partially rescued by Ank2^XL-Δall

(Stephan et al., 2015)

Ank2^f00518 is partially rescued by Ank2^XL-5xR

(Stephan et al., 2015)

Ank2^f00518 is partially rescued by Ank2^XL-20xR

(Stephan et al., 2015)

Ank2^f00518 is partially rescued by Ank2^XL-ΔR

(Stephan et al., 2015)

Comments

Ank2^XL-ΔR, Ank2^XL-20xR or Ank2^XL-5xR partially rescues the defective synapse morphology of Ank2^f00518/Ank2^f00518 mutant larvae.

Ank2^+t82 fully rescues the lethality, synaptic morphology and stability, and microtubule organization of Ank2^f00518/Ank2^f00518 mutant larvae.

Ank2^XL-Δall rescues the lethality of Ank2^f00518/Ank2^f00518 mutant larvae.

(Stephan et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

85608

w¹¹¹⁸; PBac{WH}Ank2^f00518 CG32373^f00518/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Ank2⁵¹⁸

(Migh et al., 2018)

Ank2^F00518

(Weiner et al., 2020)

Ank2^f00518

(Jegla et al., 2016, Bulat et al., 2014)

Ank2^null

(Migh et al., 2018)

ank2⁵¹⁸

(Qu et al., 2017, Siegenthaler et al., 2015, Stephan et al., 2015, Pielage et al., 2008)

ank2^f00518

(Chen et al., 2023)

ank2^null

(Stephan et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections