FlyBase Allele Report: Dmel\Arpc1[Q25sd]

General Information

Symbol

Dmel\Arpc1^Q25sd

Species

D. melanogaster

Name

FlyBase ID

FBal0135790

Feature type

allele

Associated gene

Dmel\Arpc1

Associated Insertion(s)

Carried in Construct

Also Known As

sop2^Q25sd

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Hudson and Cooley, 2002)

Progenitor genotype

Cytology

Description

A mutation in the conserved splice donor dinucleotide after Q25 (C/gt->C/at).

(Hudson and Cooley, 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:13,829,027..13,829,027 [-]

Nucleotide change:

G13829027A

Reported nucleotide change:

GT?AT

Amino acid change:

W108term | Arpc1-PA; W108term | Arpc1-PB

Comment:

A mutation in the conserved splice donor dinucleotide after Q25 (C/gt->C/at).

(Hudson and Cooley, 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage | somatic clone

(Stürner et al., 2022)

abnormal neuroanatomy | third instar larval stage

(Gokhale et al., 2016)

lethal

(Galy et al., 2011)

lethal (with Arpc1¹)

(Hudson and Cooley, 2002)

lethal (with Df(2L)b84a9)

(Hudson and Cooley, 2002)

lethal | recessive

(Hudson and Cooley, 2002, Tal et al., 2002)

Phenotype Manifest In

actin filament & nurse cell | germ-line clone

(Hudson and Cooley, 2002)

dendrite | somatic clone | third instar larval stage

(Gokhale et al., 2016)

dendritic branch | decreased number | larval stage | somatic clone

(Stürner et al., 2022)

eye | somatic clone

(Zallen et al., 2002)

filopodium (with Arpc1¹)

(Gonçalves-Pimentel et al., 2011)

interommatidial bristle | adult stage | somatic clone

(Galy et al., 2011)

interommatidial bristle | somatic clone

(Zallen et al., 2002)

larval multidendritic class III neuron | somatic clone

(Stürner et al., 2022)

larval multidendritic class IV neuron | somatic clone | third instar larval stage

(Gokhale et al., 2016)

lens | somatic clone

(Zallen et al., 2002)

macrochaeta & adult head | somatic clone

(Zallen et al., 2002)

macrochaeta | somatic clone

(Tal et al., 2002)

microchaeta & adult head | somatic clone

(Zallen et al., 2002)

NMJ bouton | increased number | third instar larval stage

(Gokhale et al., 2016)

nucleus & nurse cell | germ-line clone

(Hudson and Cooley, 2002)

nurse cell | germline clone

(Hudson and Cooley, 2002)

nurse cell ring canal | germline clone

(Hudson and Cooley, 2002)

ommatidium | adult stage | somatic clone

(Galy et al., 2011)

ommatidium | somatic clone

(Zallen et al., 2002)

photoreceptor | adult stage | somatic clone

(Galy et al., 2011)

retina | adult stage | somatic clone

(Galy et al., 2011)

rhabdomere | adult stage | somatic clone

(Galy et al., 2011)

tormogen cell | somatic clone

(Tal et al., 2002)

Detailed Description

Statement

Reference

Arpc1^Q25sd heterozygous mutant third instar larvae display increased number of boutons on neuromuscular junctions. The number of dendritic branches as well as the total dendrite length in class IV dendritic arborizing neurons in heterozygous larvae is comparable to controls but severely reduced in MARCM somatic clones homozygous for Arpc1^Q25sd.

(Gokhale et al., 2016)

Homozygous SCAR^Δ37 mutant eye clones lack interommatidial bristles. They also show an irregular ommatidia array and crater-like intrusions. Rhabdomeres appear short and bulky and photoreceptors have fallen through the fenestrated membrane, retinal depth is reduced and defects are seen in cytoskeletal architecture.

(Galy et al., 2011)

Cultured primary neurons derived from Sop2¹/Sop2^Q25sd embryos show a significant reduction in the number of filopodia compared to control neurons.

(Gonçalves-Pimentel et al., 2011)

Ring canals between nurse cells mutant for Sop2^Q25sd first begin to look different from wild-type ring canals at stage 5-6 of oogenesis: actin incorporation in mutant ring canals is less robust, and ring canals are smaller in diameter. These defects are more clear by stage 8, when some ring canals also become misshapen. By stage 10, mutant ring canals are severely deformed. In later stages, ring canals are sometimes detached from the nurse cell membranes, nurse cell subcortical actin becomes destabilized, and some nurse cells become multinucleate. This latter phenotype is not due to an inhibition of cell division, as nurse cell numbers remain normal.

(Hudson and Cooley, 2002)

When homozygous mutant clones are made in the head, a pronounced loss of external sensory organ structures is seen, including both the bristle-shafts and the socket structures from which they emanate.

(Tal et al., 2002)

CNS axon morphology in Sop2^Q25sd homozygous embryos is normal. Extensive bristle loss and a rough-eye phenotype are apparent in heads carrying somatic clones of Sop2^Q25sd. The abnormal eye facet pattern of these mosaic eyes is characterized by irregularly shaped ommatidia, craters of missing lens material, and a bristle-loss phenotype.

(Zallen et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Arpc1^Q25sd has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Blos1[+]/Blos1^ex65

(Gokhale et al., 2016)

Arpc1^Q25sd has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Pldn[+]/Pldn^Δ1

(Gokhale et al., 2016)

Arpc1^Q25sd has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Dysb[+]/Dysb^e01028

(Gokhale et al., 2016)

Enhancer of

Statement

Reference

Arpc1^Q25sd/Arpc1[+] is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Blos1^ex65

(Gokhale et al., 2016)

NOT Enhancer of

Statement

Reference

Arpc1^Q25sd/Arpc1[+] is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Dysb^e01028

(Gokhale et al., 2016)

Suppressor of

Statement

Reference

Arpc1^Q25sd/Arpc1[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Dysb^e01028

(Gokhale et al., 2016)

Arpc1^Q25sd/Arpc1[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Blos1^ex65

(Gokhale et al., 2016)

Arpc1^Q25sd/Arpc1[+] is a suppressor of abnormal cell migration phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

NOT Suppressor of

Statement

Reference

Arpc1^Q25sd/Arpc1[+] is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Dysb^e01028

(Gokhale et al., 2016)

Arpc1^Q25sd/Sop2[+] is a non-suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Other

Statement

Reference

Arpc1^Q25sd, Csk^GD9345 has lethal phenotype

(Rudrapatna et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Arpc1^Q25sd has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Blos1[+]/Blos1^ex65

(Gokhale et al., 2016)

Arpc1^Q25sd has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Pldn[+]/Pldn^Δ1

(Gokhale et al., 2016)

Arpc1^Q25sd has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Dysb[+]/Dysb^e01028

(Gokhale et al., 2016)

Enhancer of

Statement

Reference

Arpc1^Q25sd/Arpc1[+] is an enhancer of larval multidendritic class IV neuron | third instar larval stage phenotype of Blos1^ex65

(Gokhale et al., 2016)

Arpc1^Q25sd/Arpc1[+] is an enhancer of dendrite | third instar larval stage phenotype of Blos1^ex65

(Gokhale et al., 2016)

NOT Enhancer of

Statement

Reference

Arpc1^Q25sd/Arpc1[+] is a non-enhancer of larval multidendritic class IV neuron | third instar larval stage phenotype of Dysb^e01028

(Gokhale et al., 2016)

Arpc1^Q25sd/Arpc1[+] is a non-enhancer of dendrite | third instar larval stage phenotype of Dysb^e01028

(Gokhale et al., 2016)

Suppressor of

Statement

Reference

Arpc1^Q25sd/Arpc1[+] is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Dysb^e01028

(Gokhale et al., 2016)

Arpc1^Q25sd/Arpc1[+] is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Blos1^ex65

(Gokhale et al., 2016)

Arpc1^Q25sd/Arpc1[+] is a suppressor of wing disc phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

NOT Suppressor of

Statement

Reference

Arpc1^Q25sd/Arpc1[+] is a non-suppressor of larval multidendritic class IV neuron | third instar larval stage phenotype of Dysb^e01028

(Gokhale et al., 2016)

Arpc1^Q25sd/Arpc1[+] is a non-suppressor of dendrite | third instar larval stage phenotype of Dysb^e01028

(Gokhale et al., 2016)

Arpc1^Q25sd/Sop2[+] is a non-suppressor of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Other

Statement

Reference

Arpc1^Q25sd, DAAM[+]/DAAM^Ex68 has embryonic/larval central nervous system phenotype

(Gonçalves-Pimentel et al., 2011)

Arpc1^Q25sd/Sop2[+], DAAM^Ex68 has embryonic/larval central nervous system phenotype

(Gonçalves-Pimentel et al., 2011)

Arp3^EP3640, Arpc1^Q25sd/Df(2L)b84a9 has larval ventral nerve cord commissure phenotype

(Zallen et al., 2002)

Arp3^EP3640/Arp66B[+], Arpc1^Q25sd/Df(2L)b84a9 has larval ventral nerve cord commissure phenotype

(Zallen et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of boutons on neuromuscular junctions observed in third instar larvae mutant for any of the following alleles: Arpc1^Q25sd (in heterozygous state), Pldn^del (homozygous), Dysb^e01028 (either homozygous or heterozygous) or Blos1^ex65 (heterozygous) is restored to wild-type levels in Arpc1^Q25sd/+;Pldn^del/+, Arpc1^Q25sd/+;Dysb^e01028/+ or Arpc1^Q25sd/+;Blos1^ex65/+ double heterozygotes.

The reduced number of dendritic branches as well as the total dendrite length in class IV dendritic arborizing neurons characteristic for Dysb^e01028 heterozugous larvae is not modified by combination with a single copy of Arpc1^Q25sd. The reduced number of branches observed in Blos1^ex65 heterozygotes is mildly enhanced in Blos1^ex65;Arpc1^Q25sd double heterozygotes but the total dendrite length per neuron remains unchanged.

(Gokhale et al., 2016)

An Arpc1^Q25sd heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing Csk^GD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4^ptc-559.1.

(Rudrapatna et al., 2014)

Only 20% of cultured primary neurons derived from DAAM^Ex1/DAAM^Ex68 Sop2¹/Sop2^Q25sd embryos have neurites.

(Gonçalves-Pimentel et al., 2011)

In Sop2^Q25sd/Df(2L)b84a9; Arp66B^EP3640/+ embryos, commissural axons are severely reduced.

(Zallen et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Arpc1^Q25sd is rescued by Arpc1^+tPa

(Hudson and Cooley, 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9137

P{hsFLP}12, y¹ w^*; Arpc1^Q25sd P{neoFRT}40A/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

ArpC1^Q25sd

(Gerdes et al., 2020)

Arpc1^Q25sd

(Tsarouhas et al., 2019, Gokhale et al., 2016, Galy et al., 2011, Hudson and Cooley, 2002, Zallen et al., 2002)

Sop2^Q25sd

(Gonçalves-Pimentel et al., 2011)

arpc1 q25sd

(Stürner et al., 2022)

arpc1^Q25SD

(Pinheiro et al., 2017)

sop2^Q25sd

(Rudrapatna et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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