FlyBase Allele Report: Dmel\Blos1[ex65]

General Information

Symbol

Dmel\Blos1^ex65

Species

D. melanogaster

Name

FlyBase ID

FBal0242578

Feature type

allele

Associated gene

Dmel\Blos1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Cheli et al., 2010)

Progenitor genotype

P{EPgy2}Blos1^EY06269

(Cheli et al., 2010)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion which removes sequences from -56 to +1033 relative to the ATG start site.

(Cheli et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of schizophrenia

CEA

(Mullin et al., 2015)

CEA with Dysb^e01028

(Mullin et al., 2015)

model of Hermansky-Pudlak syndrome

CEA

(Cheli et al., 2010)

model of metal metabolism disorder

CEA

(Gokhale et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color | recessive

(Cheli et al., 2010)

abnormal neuroanatomy | dominant

(Mullin et al., 2015)

abnormal neuroanatomy | third instar larval stage

(Gokhale et al., 2016)

abnormal neurophysiology | dominant

(Mullin et al., 2015)

fertile

(Cheli et al., 2010)

viable

(Cheli et al., 2010)

Phenotype Manifest In

dendrite | third instar larval stage

(Gokhale et al., 2016)

larval multidendritic class IV neuron | third instar larval stage

(Gokhale et al., 2016)

NMJ bouton | increased number

(Mullin et al., 2015)

NMJ bouton | increased number | third instar larval stage

(Gokhale et al., 2016)

photoreceptor cell pigment granule

(Cheli et al., 2010)

Detailed Description

Statement

Reference

Blos1^ex65 heterozygous mutant third instar larvae display increased number of boutons on neuromuscular junctions and reduced number of dendritic branches as well as the total dendrite length in class IV dendritic arborizing neurons.

(Gokhale et al., 2016)

Both homozygous and heterozygous Blos1^ex65 mutant flies have increased miniature excitatory junction potential amplitude and a correspondingly lower quantal content.

Homozygous Blos1^ex65 mutant flies exhibit deficits in short term olfactory habituation. This phenotype is not observed in either heterozygote.

Blos1^ex65 mutants show a dominant increase in the number of boutons per muscle at the 6/7 synapse, but overall muscle area is unaffected.

(Mullin et al., 2015)

Homozygous flies show a reduction in both brown and red pigment levels in the retina, in the absence of any gross anatomical abnormality. There are no defects in the organisation of the ommatidia or in the number of photoreceptors. The number and electron density of pigment granules within mutant pigment cells is reduced, and the remaining pigment granules have an abnormal morphology.

(Cheli et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Blos1^ex65 has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Arpc1^Q25sd/Arpc1[+]

(Gokhale et al., 2016)

Suppressed by

Statement

Reference

Blos1^ex65 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Arpc1[+]/Arpc1^Q25st

(Gokhale et al., 2016)

Blos1^ex65 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Arpc1^Q25sd/Arpc1[+]

(Gokhale et al., 2016)

Blos1^ex65 has abnormal neurophysiology | dominant phenotype, suppressible by Dysb[+]/Dysb^e01028

(Mullin et al., 2015)

Blos1^ex65 has abnormal neuroanatomy | dominant phenotype, suppressible by Dysb[+]/Dysb^e01028

(Mullin et al., 2015)

NOT suppressed by

Statement

Reference

Blos1^ex65 has abnormal neuroanatomy | dominant phenotype, non-suppressible by Dysb^rv/Dysb[+]

(Mullin et al., 2015)

Suppressor of

Statement

Reference

Blos1[+]/Blos1^ex65 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Arpc1^Q25sd

(Gokhale et al., 2016)

Blos1[+]/Blos1^ex65 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Arpc1^Q25st

(Gokhale et al., 2016)

Other

Statement

Reference

Blos1[+]/Blos1^ex65, Dysb^e01028 has abnormal learning phenotype

(Mullin et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Blos1^ex65 has larval multidendritic class IV neuron | third instar larval stage phenotype, enhanceable by Arpc1^Q25sd/Arpc1[+]

(Gokhale et al., 2016)

Blos1^ex65 has dendrite | third instar larval stage phenotype, enhanceable by Arpc1^Q25sd/Arpc1[+]

(Gokhale et al., 2016)

Suppressed by

Statement

Reference

Blos1^ex65 has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Arpc1^Q25sd/Arpc1[+]

(Gokhale et al., 2016)

Blos1^ex65 has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Arpc1[+]/Arpc1^Q25st

(Gokhale et al., 2016)

Blos1^ex65 has NMJ bouton | increased number phenotype, suppressible by Dysb[+]/Dysb^e01028

(Mullin et al., 2015)

NOT suppressed by

Statement

Reference

Blos1^ex65 has NMJ bouton | increased number phenotype, non-suppressible by Dysb^rv/Dysb[+]

(Mullin et al., 2015)

Suppressor of

Statement

Reference

Blos1[+]/Blos1^ex65 is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Arpc1^Q25sd

(Gokhale et al., 2016)

Blos1[+]/Blos1^ex65 is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Arpc1^Q25st

(Gokhale et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of boutons on neuromuscular junctions observed in third instar larvae heterozygous for any of the following alleles: Arpc1^Q25sd, Arpc1^Q25st or Blos1^ex65 is restored to wild-type levels in both Arpc1^Q25sd/+;Blos1^ex65/+ and Arpc1^Q25st/+;Blos1^ex65/+ double heterozygotes.

The reduced number of dendritic branches in class IV dendritic arborizing neurons characteristic for Blos1^ex65 heterozygous larvae is mildly enhanced in Blos1^ex65;Arpc1^Q25sd double heterozygotes but the total dendrite length per neuron remains unchanged.

(Gokhale et al., 2016)

One copy of Dysb^e01028 suppresses the dominant changes in mEJP amplitude and quantal content seen in Blos1^ex65/+ mutant NMJs. The increase in bouton number seen in Blos1^ex65/+ NMJs is also suppressed. Dysb^e01028 Blos1^ex65 double heterozygotes exhibit deficits in short term olfactory habituation. This phenotype is not observed in either heterozygote alone.

One copy of Dysb^rv does not suppress the increase in bouton number seen in Blos1^ex65/+ NMJs.

(Mullin et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Blos1^ex65 is rescued by Scer\GAL4^elav-C155/Blos1^UAS.cCa

(Mullin et al., 2015)

Comments

Pre-synaptic expression of Blos1^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 rescues the changes in mEJP amplitude seen in Blos1^ex65 mutant NMJs.

(Mullin et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Blos1^ex65

(Mullin et al., 2015)

blos1^ex65

(Gokhale et al., 2016, Gokhale et al., 2015, Cheli et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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