FB2024_03 , released June 25, 2024
Allele: Dmel\enaUAS.GFP
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General Information
Symbol
Dmel\enaUAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0218985
Feature type
allele
Associated gene
Dmel\ena
Associated Insertion(s)
Carried in Construct
P{UAS-ena.GFP}
Also Known As
UAS-ena
Key Links
Transgenic product class
wild_type
(Gates et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Gates et al., 2007)
Mutagen
in vitro construct
(Gates et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-ena.GFP}
Cytology
Description

The fluorescent tag is appended to the N-terminus of ena.

(Nowotarsky and Peifer, 2014.10.31)

UAS regulatory sequences drive expression of ena which is tagged with GFP.

(Gates et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
ena
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Border cell clusters expressing enaScer\UAS.T:Avic\GFP under the control of Scer\GAL4slbo.2.6 have many fine protrusions at the beginning of migration, in contrast to wild type, where one prominent protrusion is usually seen in the direction of the oocyte. Border cell migration is defective in these mutants.

      (Gates et al., 2009)

      Expression of enaScer\UAS.T:Avic\GFP under the control of Scer\GAL4en-e16E can increase filopodia number and length at the leading edge and larger lamellipodia are seen.

      (Homem and Peifer, 2009)

      Expression of enaScer\UAS.T:Avic\GFP under the control of Scer\GAL4e22c does not result in embryonic lethality.

      Leading edge cells in embryos expressing enaScer\UAS.T:Avic\GFP under the control of Scer\GAL4en-e16E produce longer filopodia than wild type. Lateral epithelial cells produce many filopodia in these embryos (these cells do not produce filopodia in wild-type embryos).

      (Gates et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      enaUAS.GFP/Scer\GAL415J2 is a suppressor of abnormal neuroanatomy | recessive phenotype of Nl1N-ts1

      (Kuzina et al., 2011)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of enaScer\UAS.T:Avic\GFP under the control of Scer\GAL415J2 suppresses the early longitudinal axon phenotype of Nl1N-ts1.

      (Kuzina et al., 2011)

      Co-expression of enaScer\UAS.T:Avic\GFP and diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E in embryos induces protrusions in epidermal cells that are different from those produced by expression of either construct alone. Co-expression results in lamellipodia both at the leading edge and in more ventral epidermal cells, at the expense of filopodia.

      Co-expression of enaScer\UAS.T:Avic\GFP and diaDDFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E in embryos results in fewer filopodia but larger lamellipodia at the leading edge.

      Co-expression of enaScer\UAS.T:Avic\GFP and diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E in embryos results in fewer filopodia but larger lamellipodia at the leading edge.

      (Homem and Peifer, 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      enaUAS.GFP/Scer\GAL4how-24B partially rescues enaGC5

      (King et al., 2021)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      enaScer\UAS.T:Avic\GFP
      enaUAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (7)