FB2024_03 , released June 25, 2024
Allele: Dmel\diaDDFH1FH2.UASp.EGFP
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General Information
Symbol
Dmel\diaDDFH1FH2.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0277862
Feature type
allele
Associated gene
Dmel\dia
Associated Insertion(s)
Carried in Construct
P{UASp-dia.DDFH1FH2.EGFP}
Key Links
Transgenic product class
inframe_deletion
(Homem and Peifer, 2009)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Homem and Peifer, 2009)
Mutagen
in vitro construct
(Homem and Peifer, 2009)
Progenitor genotype
Carried in construct
P{UASp-dia.DDFH1FH2.EGFP}
Cytology
Description

UASp regulatory sequences drive expression of the dimerisation (DD), FH1 and FH2 domains of dia. The protein is tagged at the N-terminal end with EGFP (the tag is joined to the dia coding region via a HRYTSLYKKAGSAAAPFT linker).

(Homem and Peifer, 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
dia
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of diaDDFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E does not induce significant numbers of ectopic filopodia at the leading edge and there is little effect on protrusions of epidermal cells ventral to the leading edge. Lamellipodial area and filopodial number are significantly increased in leading edge cells. Filopodial lifetime is significantly increased compared to wild type.

      (Homem and Peifer, 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of enaScer\UAS.T:Avic\GFP and diaDDFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E in embryos results in fewer filopodia but larger lamellipodia at the leading edge.

      (Homem and Peifer, 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      diaDDFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP
      diaDDFH1FH2.UASp.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)