FB2024_03 , released June 25, 2024
Allele: Dmel\enaC14-06
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General Information
Symbol
Dmel\enaC14-06
Species
D. melanogaster
Name
FlyBase ID
FBal0282342
Feature type
allele
Associated gene
Dmel\ena
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Sano et al., 2012)
Mutagen
ethyl methanesulfonate
(Sano et al., 2012)
Progenitor genotype
Cytology
Description

Nucleotide substitution: C?T.

(Sano et al., 2012)

Amino acid replacement: Q388term.

(Sano et al., 2012)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:19,160,540..19,160,540 [+]
Nucleotide change:

C19160540T

Reported nucleotide change:

C?T

Amino acid change:

Q389term | ena-PA; Q538term | ena-PB; Q389term | ena-PC; Q392term | ena-PD; Q389term | ena-PE; Q685term | ena-PF; Q369term | ena-PG

Reported amino acid change:

Q388term

(Sano et al., 2012)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Somatic gonadal precursors (SGPs) are specified normally as three clusters and primordial germ cells (PGCs) are associated with all three clusters in homozygous embryos. However, these clusters fail to adhere to each other at stage 13, and the mutant gonads remain elongated or split at stage 16 in the mutant embryos. Some mis-migrating PGCs are also seen outside the gonad from stage 13 onwards.

      The number of SGP protrusions appear to be normal in mutant gonads at stage 15-16, extending between the PGCs as in wild type. The changes in SGP cell morphology and cell orientation that normally happen during gonad compaction do not occur in the mutant embryos.

      (Sano et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT suppressed by
      Statement
      Reference

      enaC14-06 has gonad | embryonic stage phenotype, non-suppressible | maternal effect by osk8/oskCE4

      (Sano et al., 2012)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Embryos that are derived from oskCE4/osk8 females and are zygotically homozygous for enaC14-06 show defects or delays in somatic gonadal precursor cluster adhesion at stage 13 and fail to complete gonad compaction, resulting in extended or split gonads at later stages, as occurs in enaC14-06 single mutant embryos.

      67.8% of gonads do not form correctly in enaC14-06/+, shgg317/+ transheterozygous embryos, compared to 28.8% in enaC14-06/+ and 37.3% in shgg317/+ single heterozygotes.

      More than 50% of gonads do not form correctly in enaC14-06/+, shg2/+ transheterozygous embryos.

      51.9% of gonads fail to compact properly in enaC14-06/+, zip1/+ transheterozygous embryos, compared to 33.3% in zip1/+ single heterozygotes.

      59.8% of gonads fail to compact properly in enaC14-06/+, arm1/+ transheterozygous embryos, compared to 22.1 in arm1/+ single heterozygotes.

      (Sano et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      enaC14-06 is partially rescued by Scer\GAL4how-24B/enaUAS.cGa

      (Sano et al., 2012)
      Not rescued by

      enaC14-06 is not rescued by Scer\GAL4VP16.nanos.UTR/enaUAS.cGa

      (Sano et al., 2012)
      Comments

      Expression of enaScer\UAS.cGa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the gonad coalescence defects seen in enaC14-06 embryos.

      Expression of enaScer\UAS.cGa under the control of Scer\GAL4how-24B partially rescues the gonad coalescence defects seen in enaC14-06 embryos.

      (Sano et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      enaC14-06
      (Sano et al., 2012)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)