FlyBase Allele Report: Dmel\shg[g317]

General Information

Symbol

Dmel\shg^g317

Species

D. melanogaster

Name

FlyBase ID

FBal0028554

Feature type

allele

Associated gene

Dmel\shg

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

gain of function allele

Mutagen

gamma ray

Nature of the Allele

Allele class

gain of function allele

(Tepass et al., 1996)

Mutagen

gamma ray

(Tepass et al., 1996)

Progenitor genotype

Caused by aberration

In(2R)shg^g317

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion | maternal effect

(Degennaro et al., 2011)

abnormal cell migration | maternal effect

(Degennaro et al., 2011)

lethal - all die during embryonic stage

(Tepass et al., 1996)

some die during embryonic stage

(Tepass et al., 1996)

Phenotype Manifest In

actin filament | embryonic stage

(Jurado et al., 2016)

amnioserosa

(Jurado et al., 2016)

egg | germline clone

(Tepass et al., 1996)

embryonic epidermis

(Tepass et al., 1996)

endoderm

(Tepass and Hartenstein, 1994)

fat body/gonad primordium

(Jenkins et al., 2003)

fat body/gonad primordium | male

(Jenkins et al., 2003)

germline cell | embryonic stage | maternal effect

(Jenkins et al., 2003)

germline cell | maternal effect

(Degennaro et al., 2011)

gonadal sheath proper primordium

(Jenkins et al., 2003)

gonadal sheath proper primordium | male

(Jenkins et al., 2003)

Malpighian tubule

(Tepass et al., 1996)

neurogenic region

(Tepass et al., 1996)

ovary | germline clone

(Tepass et al., 1996)

presumptive embryonic/larval peripheral nervous system

(Tepass et al., 1996)

stomatogastric nervous system | embryonic stage

(Tepass et al., 1996)

visual primordium

(Tepass et al., 1996)

Detailed Description

Statement

Reference

shg^g317 mutant embryos display defects in the actin dynamics in the amnioserosa cells compared to controls.

(Jurado et al., 2016)

In embryos from females heterozygous for shg^g317, an average of 3.7 PGCs are left outside the midgut with 64% of embryos exhibiting a phenotype.

(Degennaro et al., 2011)

In shg^g317 homozygous embryos gonad compaction is sometimes initiated, but often does not proceed to completion. The three clusters of somatic gonadal precursors (SGPs) from parasegments (PS) 10-12 are able to associate correctly with one another, and with germ cells, to form a cohesive group. However, these cells often remain loosely associated and spread over more than one parasegment, rather than compacting tightly in PS10. In the most severe cases, compaction from PS10-12 to PS10 appears completely blocked. In weaker examples, compaction is initiated but not completed, resulting in partially compacted and misshapen gonads. During ensheathment of germ cells by gonadal mesoderm in these embryos the gonadal mesoderm cells fail to make cellular extensions between and around the germ cells. Phenotypes are stronger in these embryos than in shg² homozygotes. This is true, both for the compaction phenotype (22% severe, 41% weak, n=49 in shg^g317 homozygotes; 3% severe, 50% weak, n=36 in shg² homozygotes) and the ensheathment phenotype, but the severity of these two phenotypes do not correlate with each other. In male shg^g317 homozygous embryos male specific gonadal precursors often fail to join the posterior of the male gonad (42% male embryos n=49). Offspring from heterozygous shg^g317/+ females are viable, but the embryos have a significant germ cell migration defect. Offspring from the reciprocal cross using shg^g317/+ males show no germ cell migration defect.

(Jenkins et al., 2003)

Class IV allele: mutants lack all head and ventral cuticle, and show segmental defects in lateral epidermis or dorsal and lateral epidermis. Defects are evident in the neurectoderm, Malpighian tubules, optic lobe precursor, PNS and SNS. In germ-line clones, no eggs are recovered.

(Tepass et al., 1996)

The principal midgut epithelial cells spread over the visceral mesoderm but do not become columnar, they maintain a rounded to cuboidal shape, and do not form a monolayer. At later stages (stage 14-17) the principle midgut epithelial cells become attached to the visceral mesoderm and gradually adopt a more wild type appearance. By the time the embryos are fully differentiated, no difference from wild type can be seen.

(Tepass and Hartenstein, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

shg^g317 has abnormal cell migration | maternal effect phenotype, enhanceable by Jafrac1[+]/Prx2^KG05372

(Degennaro et al., 2011)

shg^g317 has abnormal cell adhesion | maternal effect phenotype, enhanceable by Jafrac1[+]/Prx2^KG05372

(Degennaro et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

shg^g317 has germline cell | maternal effect phenotype, enhanceable by Jafrac1[+]/Prx2^KG05372

(Degennaro et al., 2011)

Suppressed by

Statement

Reference

shg^g317 has neurogenic region phenotype, suppressible by N^int.hs

(Tepass et al., 1996)

shg^g317 has Malpighian tubule phenotype, suppressible by ct^C145

(Tepass et al., 1996)

Enhancer of

Statement

Reference

shg[+]/shg^g317 is an enhancer of germline cell | embryonic stage 15 phenotype of raw^155.27/raw^134.47

(Jemc et al., 2012)

shg^g317 is an enhancer of germline cell | embryonic stage 15 phenotype of raw^155.27/raw^134.47

(Jemc et al., 2012)

shg[+]/shg^g317 is an enhancer of adult thorax phenotype of Src42A^6-1

(Takahashi et al., 2005)

Other

Statement

Reference

ena[+]/ena^C14-06, shg^g317 has gonad | embryonic stage phenotype

(Sano et al., 2012)

ena^C14-06, shg[+]/shg^g317 has gonad | embryonic stage phenotype

(Sano et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

shg^g317/+ enhances the frequency of ensheathment defects in raw^134.47/raw^155.27 mutants. Homozygous shg^g317 does so even at a greater frequency.

(Jemc et al., 2012)

67.8% of gonads do not form correctly in ena^C14-06/+, shg^g317/+ transheterozygous embryos, compared to 28.8% in ena^C14-06/+ and 37.3% in shg^g317/+ single heterozygotes.

(Sano et al., 2012)

Embryos from Jafrac1^KG05372, shg^g317 trans-heterozygous mothers display a significantly enhanced primordial germline cell adherence defect compared to single shg^g317 mutants. These mothers produce embryos with an average of 6.2 primordial germline cells left outside of the midgut with 91% of embryos affected.

(Degennaro et al., 2011)

Src42A^6-1 notum phenotypes are enhanced in a shg^g317/+ background; in some Src42A^6-1; shg^g317/+ flies the right and left halves of the notum appear completely separated.

(Takahashi et al., 2005)

Neurectoderm defect is rescued by N^int.hs. Malpighian tubule defect of shg^g317 is rescued by ct^C145. The zonula adherens of many epithelial cells is unaffected by zygotic loss of shg function.

(Tepass et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(2R)E2/shg^g317 is rescued by shg^{Ubi-p63E.sgGFP}

(Oda and Tsukita, 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Strong allele. Visceral mesoderm develops normally.

(Tepass and Hartenstein, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

shg ^g317

(Jemc et al., 2012)

shg^G137

(Jenkins et al., 2003)

shg^G317

(Sano et al., 2012, Noll et al., 2000)

shg^g317

(Jurado et al., 2016, Li et al., 2015, Jemc et al., 2012, Degennaro et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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