FlyBase Allele Report: Dmel\Prx2[KG05372]

General Information

Symbol

Dmel\Prx2^KG05372

Species

D. melanogaster

Name

FlyBase ID

FBal0147828

Feature type

allele

Associated gene

Dmel\Prx2

Associated Insertion(s)

P{SUPor-P}Prx2^KG05372

Carried in Construct

Also Known As

Jafrac1^KG05372

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Degennaro et al., 2011)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}Prx2^KG05372

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:13,330,006..13,330,006 [-]

(Chew et al., 2021, Degennaro et al., 2011, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion

(Degennaro et al., 2011)

abnormal cell adhesion (with Prx2^f08066)

(Degennaro et al., 2011)

abnormal cell migration

(Degennaro et al., 2011)

abnormal cell migration (with Prx2^f08066)

(Degennaro et al., 2011)

abnormal oxidative stress response

(Degennaro et al., 2011)

abnormal starvation stress response

(Degennaro et al., 2011)

Phenotype Manifest In

germline cell | maternal effect

(Degennaro et al., 2011)

germline cell | maternal effect (with Prx2^f08066)

(Degennaro et al., 2011)

Detailed Description

Statement

Reference

Jafrac1^KG05372/Jafrac1^KG05372 flies do not show significant differences to controls under exposure to oxidative stress (10mM paraquat).

(Khoshnood et al., 2016)

Germ cells from Jafrac1^KG05372 mutant mothers are left outside of the midgut during gastrulation. 73% of embryos from mothers homozygous for Jafrac1^KG05372 display this phenotype, with an average of 6 germ cells left outside the midgut per embryo. Jafrac1^KG05372/Jafrac1^f08066 mutants exhibit an intermediate phenotype between the two alleles.

Jafrac1^KG05372 flies exposed to paraquat are less viable compared to wild-type flies, suggesting an increased sensitivity to oxidative stress in the mutant.

Jafrac1^KG05372 mutants are homozygous viable and fertile.

Adult Jafrac1^KG05372 flies can tolerate dry starvation better than wild-type controls.

Jafrac1^KG05372 mutant mothers form a similar number of primordial germline cells (PGC) outside and inside the gut as in heterozygous and wild-type controls, suggesting that Jafrac1 is not required for PGC survival.

Jafrac1^KG05372 mutant primordial germline cells that enter the midgut during gastrulation polarize prior to migrating through the midgut epithelium and subsequently move into the mesoderm and reach the somatic gonadal precursors similarly to wild-type or heterozygous controls.

Jafrac1^KG05372 mutant primordial germline cells that are left outside the embryo during gastrulation remain there throughout embryogenesis and retain their normal morphology.

There are no obvious differences between Jafrac1^KG05372 and wild-type primordial germline cells in protrusion formation at this early stage. Once gastrulation is initiated, Jafrac1^KG05372 primordial germline cells are more amoeboid in character and less tightly associated with the soma and each other (compared to wild-type controls), preventing stable cluster formation. As gastrulation proceeds, primordial germline cells that are not close to the center of the invaginating midgut primordium in Jafrac1^KG05372 mutant embryos remain in the hindgut or outside the embryo, instead of being clustered within the midgut. Jafrac1^KG05372/Jafrac1^f08066 mutants exhibit an intermediate phenotype between the two alleles.

Embryos from Jafrac1^KG05372 heterozygous mothers exhibit normal primordial germline cell adhesion during gastrulation.

(Degennaro et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Jafrac1[+]/Prx2^KG05372 is an enhancer of abnormal cell migration | maternal effect phenotype of shg^g317

(Degennaro et al., 2011)

Jafrac1[+]/Prx2^KG05372 is an enhancer of abnormal cell adhesion | maternal effect phenotype of shg^g317

(Degennaro et al., 2011)

Jafrac1[+]/Prx2^KG05372 is an enhancer of abnormal cell migration | maternal effect phenotype of shg²

(Degennaro et al., 2011)

Jafrac1[+]/Prx2^KG05372 is an enhancer of abnormal cell adhesion | maternal effect phenotype of shg²

(Degennaro et al., 2011)

Jafrac1[+]/Prx2^KG05372 is an enhancer of abnormal cell migration | maternal effect phenotype of shg³⁷³

(Degennaro et al., 2011)

Jafrac1[+]/Prx2^KG05372 is an enhancer of abnormal cell adhesion | maternal effect phenotype of shg³⁷³

(Degennaro et al., 2011)

Suppressor of

Statement

Reference

Prx2^KG05372/Prx2^KG05372 is a suppressor | partially of abnormal oxidative stress response phenotype of Urm1ⁿ¹²³

(Khoshnood et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

Jafrac1[+]/Prx2^KG05372 is an enhancer of germline cell | maternal effect phenotype of shg^g317

(Degennaro et al., 2011)

Jafrac1[+]/Prx2^KG05372 is an enhancer of germline cell | maternal effect phenotype of shg²

(Degennaro et al., 2011)

Jafrac1[+]/Prx2^KG05372 is an enhancer of germline cell | maternal effect phenotype of shg³⁷³

(Degennaro et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Jafrac1^KG05372/Jafrac1^KG05372 significantly suppresses the resistance to oxidative stress (increased survival under 10mM paraquat conditions) seen in Urm1ⁿ¹²³/Urm1ⁿ¹²³ flies.

(Khoshnood et al., 2016)

Embryos from Jafrac1^KG05372, shg^g317 trans-heterozygous mothers display a significantly enhanced primordial germline cell adherence defect compared to single shg^g317 mutants. These mothers produce embryos with an average of 6.2 primordial germline cells left outside of the midgut with 91% of embryos affected.

Embryos from Jafrac1^KG05372, shg³⁷³ trans-heterozygous mothers display a significantly enhanced primordial germline cell adherence defect compared to single shg³⁷³ mutants. These mothers produce embryos with an average of 5.4 primordial germline cells left outside of the midgut with 93% of embryos affected.

Embryos from Jafrac1^KG05372, shg² trans-heterozygous mothers display a statistically insignificant enhancement of the primordial germline cell adherence defect compared to single shg² mutants. These mothers produce embryos with an average of 0.9 primordial germline cells left outside of the midgut with 26% of embryos affected.

The presence of shg^nos.PS in a Jafrac1^KG05372 mutant background rescues the Jafrac1^KG05372 primordial germline cell adhesion defect to an average of 0.5 primordial germline cells left outside of the midgut with 21% of embryos affected. This result is statistically similar to heterozygous mutant controls with an average of 0.3 primordial germline cells left outside of the midgut with 2% of embryos affected and indicates that shg acts downstream of Jafrac1 in primordial germline cells to regulate their adhesion during gastrulation.

(Degennaro et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Prx2^KG05372 is rescued by Scer\GAL4^nanos.PG/Prx2^UAS.cDa

(Degennaro et al., 2011)

Prx2^KG05372 is rescued by Prx2^UASp.cDa/Scer\GAL4^Act5C.PI

(Degennaro et al., 2011)

Partially rescued by

Prx2^KG05372 is partially rescued by Prx2^{nanos.3'UTR-pgc}

(Degennaro et al., 2011)

Not rescued by

Prx2^KG05372 is not rescued by Scer\GAL4^nullo.PG/Prx2^UAS.cDa

(Degennaro et al., 2011)

Comments

Expression of Jafrac1^Scer\UAS.cDa in Jafrac1^KG05372 mutants under the control of Scer\GAL4^nos.PG reduces the penetrance of the primordial germline cell adhesion defect phenotype in Jafrac1^KG05372 mutants from 73% of embryos to 8% of embryos, and a reduction in the average number of primordial germline cells left outside the midgut from 6 to 0.4.

Somatic expression of Jafrac1^Scer\UAS.cDa under the control of Scer\GAL4^nullo.PG does not significantly alter the Jafrac1^KG05372 mutant phenotype, with an average of 7.2 PGCs left out of the midgut.

Ubiquitous expression of Jafrac1^{Scer\UAS.P\T.cDa} under the control of Scer\GAL4^Act5C.PI rescues the paraquat sensitivity found in Jafrac1^KG05372 mutants.

(Degennaro et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

14440

y¹ P{SUPor-P}Prx2^KG05372

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Jafrac1^KG05372

(Chew et al., 2021, Khoshnood et al., 2016)

Prx2^KG05372

Name Synonyms

Secondary FlyBase IDs

References (4)

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