FlyBase Allele Report: Dmel\crb[Y10A]

General Information

Symbol

Dmel\crb^Y10A

Species

D. melanogaster

Name

FlyBase ID

FBal0287561

Feature type

allele

Associated gene

Dmel\crb

Associated Insertion(s)

Carried in Construct

P{foscrb.Y10A}

Key Links

Transgenic product class

missense_variant

(Klose et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Klose et al., 2013)

Mutagen

in vitro construct

(Klose et al., 2013)

Progenitor genotype

crb^+tfos

(Klose et al., 2013)

Carried in construct

P{foscrb.Y10A}

Cytology

Description

Amino acid replacement: Y10A.

(Klose et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

crb

Encoded product / tool

crb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | embryonic stage, with crb^GX24w-

(Flores-Benitez and Knust, 2015)

majority die during embryonic stage, with crb^GX24w-

(Flores-Benitez and Knust, 2015)

majority die during embryonic stage, with crb^GX24w-/crb^11A22

(Flores-Benitez and Knust, 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

A majority of embryos expressing crb^Y10A in a crb^GX24w-/crb^GX24w- background exhibit lethality due to dorsal closure defects, ranging in severity from open cuticle, dorsal holes, closed cuticles but failure to hatch, or kinked larvae. These embryos also exhibit major defects in germ band retraction; a highly disorganized amnioserosa, with an increase in large processes, an increase in the number of apoptotic cells, and aberrant contractions of amnioserosal cells; and exhibit disorganization of elongation of the dorsal-most epidermal cells, and erratic extension of the filopodia extended by these cells. The amnioserosa in these embryos shows fragmentation of the zonula adherens.

A majority of embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background exhibit lethality due to dorsal closure defects, ranging in severity from open cuticle, dorsal holes, closed cuticles but failure to hatch, or kinked larvae.

(Flores-Benitez and Knust, 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, enhanceable by flw⁶/flw⁶

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, enhanceable by flw⁶

(Flores-Benitez and Knust, 2015)

Suppressed by

Statement

Reference

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, suppressible by flw^UAS.Tag:HA/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, suppressible | partially by Rho1^N19.UAS/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, suppressible | partially by Rok^CAT-KG.UAS/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-, crb^Y10A has majority die during embryonic stage phenotype, suppressible | partially by Rho1^1B

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, suppressible | partially by Pak^{AID.UAS.Tag:FLAG}/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, suppressible | partially by Moe^{K.T559D.UAS.Tag:MYC}/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, suppressible | partially by shg^UAS.cSa/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-, crb^Y10A has majority die during embryonic stage phenotype, suppressible | partially by SCAR^Δ37/SCAR[+]

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, suppressible | partially by Arp3^EP3640/Arp3[+]

(Flores-Benitez and Knust, 2015)

NOT suppressed by

Statement

Reference

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, non-suppressible by Rok²/Rok²

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, non-suppressible by Rok²

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, non-suppressible by Rac1^N17.UAS/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, non-suppressible by Moe^UAS.Tag:MYC/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has majority die during embryonic stage phenotype, non-suppressible by Arpc1[+]/Arpc1^Q25st

(Flores-Benitez and Knust, 2015)

Other

Statement

Reference

SCAR^Δ37/SCAR[+], crb^GX24w-, crb^Y10A has visible phenotype

(Flores-Benitez and Knust, 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, enhanceable by flw⁶/flw⁶

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, enhanceable by flw⁶/flw⁶

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, enhanceable by flw⁶

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, enhanceable by flw⁶

(Flores-Benitez and Knust, 2015)

Suppressed by

Statement

Reference

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, suppressible | partially by flw^UAS.Tag:HA/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, suppressible | partially by Rho1^N19.UAS/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, suppressible | partially by Rho1^N19.UAS/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, suppressible | partially by Rok^CAT-KG.UAS/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, suppressible | partially by Rok^CAT-KG.UAS/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-, crb^Y10A has embryo | dorsal closure stage phenotype, suppressible | partially by Rho1^1B

(Flores-Benitez and Knust, 2015)

crb^GX24w-, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, suppressible | partially by Rho1^1B

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, suppressible | partially by Pak^{AID.UAS.Tag:FLAG}/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, suppressible | partially by Pak^{AID.UAS.Tag:FLAG}/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, suppressible | partially by Moe^{K.T559D.UAS.Tag:MYC}/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, suppressible | partially by Moe^{K.T559D.UAS.Tag:MYC}/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, suppressible | partially by shg^UAS.cSa/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, suppressible | partially by shg^UAS.cSa/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-, crb^Y10A has embryo | dorsal closure stage phenotype, suppressible | partially by SCAR^Δ37/SCAR[+]

(Flores-Benitez and Knust, 2015)

crb^GX24w-, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, suppressible | partially by SCAR^Δ37/SCAR[+]

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, suppressible | partially by Arp3^EP3640/Arp3[+]

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, suppressible | partially by Arp3^EP3640/Arp3[+]

(Flores-Benitez and Knust, 2015)

NOT suppressed by

Statement

Reference

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, non-suppressible by Rok²

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, non-suppressible by Rac1^N17.UAS/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, non-suppressible by Rac1^N17.UAS/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, non-suppressible by Moe^UAS.Tag:MYC/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, non-suppressible by Moe^UAS.Tag:MYC/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, non-suppressible by Arpc1[+]/Arpc1^Q25st

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, non-suppressible by Arpc1[+]/Arpc1^Q25st

(Flores-Benitez and Knust, 2015)

crb^GX24w-, crb^Y10A has amnioserosa phenotype, non-suppressible by yrt⁷⁵/yrt⁷⁵

(Flores-Benitez and Knust, 2015)

crb^GX24w-, crb^Y10A has germ band | embryonic stage phenotype, non-suppressible by yrt⁷⁵/yrt⁷⁵

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, non-suppressible by Rok²/Rok²

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryonic/first instar larval cuticle | embryonic stage phenotype, non-suppressible by Rok²/Rok²

(Flores-Benitez and Knust, 2015)

crb^GX24w-/crb^11A22, crb^Y10A has embryo | dorsal closure stage phenotype, non-suppressible by Rok²

(Flores-Benitez and Knust, 2015)

Other

Statement

Reference

SCAR^Δ37/SCAR[+], crb^GX24w-, crb^Y10A has adult abdomen phenotype

(Flores-Benitez and Knust, 2015)

Scer\GAL4^332.3, crb^GX24w-/crb^11A22, crb^Y10A, flw^UAS.Tag:HA has embryonic dorsal epidermis phenotype

(Flores-Benitez and Knust, 2015)

Additional Comments

Genetic Interactions

Statement

Reference

yrt⁷⁵/yrt⁷⁵ fails to ameliorate the germ band retraction and amnioserosal integrity defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^GX24w- background.

Expression of flw^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^332.3 largely suppresses the embryonic lethality and dorsal closure defects in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background, although some embryos exhibit irregular zippering at the posterior canthus and bunching of the epidermis.

flw⁶/flw⁶ or flw⁶ hemizygosity enhances the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background.

Arp3^EP3640/+, or expression of Rho1^N19.Scer\UAS, Rok^{CAT-KG.Scer\UAS} or Moe^{K.T559D.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^332.3 partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background.

Rho1^1B hemizygosity partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^GX24w- background.

Arpc1^Q25st/+, Rok²/Rok² or Rok² hemizygosity, or expression of Rac1^N17.Scer\UAS or Moe^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^332.3, fails to suppress the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background.

Expression of Pak^{AID.Scer\UAS.T:Zzzz\FLAG} or shg^Scer\UAS.cSa under the control of Scer\GAL4^332.3 partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background, resulting in some adult survival.

SCAR^Δ37/+ partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^GX24w- background, resulting in some adults surviving, although these adults show defects in abdominal development.

(Flores-Benitez and Knust, 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Not rescued by

crb[-]/crb^Y10A is not rescued by crb^UAS.cWa/Scer\GAL4^332.3

(Flores-Benitez and Knust, 2015)

Partially rescues

crb^Y10A partially rescues crb^GX24w-

(Klose et al., 2013)

Comments

Expression of crb^Scer\UAS.cWa under the control of Scer\GAL4^332.3 fails to significantly rescue the lethality and dorsal closure defects in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background.

(Flores-Benitez and Knust, 2015)

crb^GX24w- embryos carrying two copies of crb^Y10A fail to hatch. Some rescue of the embryo cuticle phenotype is seen, with the embryos forming continuous anterior and ventral cuticle with rather fully developed denticle belts. The amnioserosa shows defects from stage 13 onwards and is completely lost at stage 15. The embryos show impaired germ band retraction, head involution and dorsal closure. The leading edge of the epidermis appear uneven and wiggling during dorsal closure. Segments show variable widths.

(Klose et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99604

w^*; P{foscrb.Y10A}attP40/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

crb^Y10A

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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