Imprecise excision of P{EPgy2}yrtEY01443 results in a 4.8kb deletion of genomic DNA, including the 5'UTR and 1080 bp of the yrt open reading frame.
Estimated boundaries of a 4.8kb deletion resulting from the imprecise excision of P{EPgy2}yrtEY01443 that is reported to remove the yrt 5' UTR and 1080bp of the yrt ORF.
yrt75 maternal-zygotic mutant stage 11 and 13 embryos and yrt75 maternal mutant stage 11 and 13 embryos that carry a paternal yrtF281R.W283R allele show epithelial polarity defects, as shown by the mislocalization of the apical Crb protein to the lateral membrane of epidermal cells; this is accompanied by severe epithelial tissue defects, as shown by lack of head cuticle, a large hole in the dorsal cuticle, and a ventral cuticle that lacks denticle belts and is highly convoluted.
Maternal/zygotic yrt75 mutant embryos display a multilayered epidermis and trachea at stage 13. The cuticle appears abnormally in-folded and convoluted. The ventral cuticle is highly in-folded and occupies a larger surface area compared to wild-type.
The apical membrane of yrt75 eye photoreceptor cells is enlarged compared to controls. Stalk length is longer than in controls, and elongation of the stalk progresses over time resulting in a much longer stalk in comparison to wild-type photoreceptor cells. yrt75 mutant eyes show normal external morphology. Photoreceptor cells display mild defects in adherens junction positioning, and in rhabdomere shape, including enlarged cross-section profiles and split rhabdomeres. No significant shortening in the proximo-distal length of rhabdomeres or a fragmentation of the zonula adherens is observed in mutant photoreceptor cells.
Massive photoreceptor cell degeneration is observed in yrt75 mutant eyes after 7 days of constant light exposure.
Scer\GAL4da.G32, aPKCUAS.Tag:CAAX(Unk), yrt75 has abnormal cell polarity phenotype
yrt75 has embryonic/first instar larval cuticle phenotype, non-suppressible by dlg114
yrt75 is a suppressor of embryonic/first instar larval cuticle phenotype of crbS87-2
yrt75 is a suppressor of embryonic/first instar larval cuticle phenotype of crb11A22
yrt75 is a suppressor of embryonic/first instar larval cuticle | germline clone phenotype of crb11A22
yrt75/yrt75 is a non-suppressor of amnioserosa phenotype of crbGX24w-, crbY10A
yrt75 is a non-suppressor of eye photoreceptor cell stalk phenotype of crb11A22
crb+tfos, crbGX24w-, yrt75 has embryo | dorsal closure stage phenotype
Scer\GAL4da.G32, aPKCUAS.Tag:CAAX(Unk), yrt75 has embryonic/larval cuticle phenotype
Scer\GAL4da.G32, aPKCUAS.Tag:CAAX(Unk), yrt75 has epithelial cell phenotype
Embryos expressing crb+tfos in a crbGX24w-/crbGX24w-, yrt75/yrt75 background exhibit defects in dorsal closure mainly after germ band retraction, although overall amnioserosa integrity is preserved during dorsal closure and most of the zippering is completed, leaving a hole only at the posterior canthus.
yrt75/yrt75 fails to ameliorate the germ band retraction and amnioserosal integrity defects seen in embryos expressing crbY10A in a crbGX24w-/crbGX24w- background.
Expression of aPKCCAAXWT.Scer\UAS in a homozygous yrt75a background results in a striking apicalisation phenotype, characterised by extreme extension of the apical membrane of epidermal cells leading to the formation of inverted cysts toward the end of embryogenesis. Consequently, the larval cuticle, secreted through the apical domain, forms little spheres typical of apicalised epidermal cells.
yrt75, crb2 double mutant photoreceptor cells show shortened stalk membranes. There is no significant difference between stalk length in double mutants compared to crb2 single mutants.
yrt75 suppresses the cuticle phenotype of crb2 (maternal or zygotic) or crbS87-2 (zygotic) mutant embryos. Defects in junctional integrity and cellular organization of the epidermis are strongly rescued in double mutants compared to crb2 or crbS87-2 mutant embryos.
The yrt75 cuticle phenotype is not modified in yrt75, dlg114 double zygotic mutant embryos.
