FB2024_03 , released June 25, 2024
Allele: Dmel\crb+tfos
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General Information
Symbol
Dmel\crb+tfos
Species
D. melanogaster
Name
FlyBase ID
FBal0287558
Feature type
allele
Associated gene
Dmel\crb
Associated Insertion(s)
Carried in Construct
P{foscrb}
Also Known As
foscrb
Key Links
Transgenic product class
genomic_DNA
(Klose et al., 2013)
wild_type
(Klose et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Klose et al., 2013)
wild_type
(Klose et al., 2013)
Mutagen
in vitro construct
(Klose et al., 2013)
Progenitor genotype
Carried in construct
P{foscrb}
Cytology
Description

Genomic fragment (fosmid P52GD2) containing the complete crb transcription unit plus approximately 7kb upstream and 5kb downstream sequence.

(Klose et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
crb
Encoded product / tool
crb
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos expressing crb+tfos in a crbGX24w-/crbGX24w- background successfully complete dorsal closure, and exhibit normal elongation and filpodia extension of the dorsal-most epidermal cells.

      (Flores-Benitez and Knust, 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of flwScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4332.3 does not lead to any obvious phenotype in embryos expressing crb+tfos in a crbGX24w-/crb11A22 background.

      SCARΔ37/+ or Arp3EP3640/+ does not lead to any dorsal closure defects in embryos expressing crb+tfos in a crbGX24w-/crb11A22 background.

      Expression of PakScer\UAS.T:Myr-Src64B under the control of Scer\GAL4332.3 in embryos expressing crb+tfos in a crbGX24w-/crb11A22 background leads to complete embryonic lethality with most embryos exhibiting dorsal closure defects ranging in severity from open cuticle and dorsal holes, to closed cuticles but failure to hatch.

      Embryos expressing crb+tfos in a crbGX24w-/crbGX24w-, yrt75/yrt75 background exhibit defects in dorsal closure mainly after germ band retraction, although overall amnioserosa integrity is preserved during dorsal closure and most of the zippering is completed, leaving a hole only at the posterior canthus.

      (Flores-Benitez and Knust, 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      crb+tfos partially rescues crbGX24w-

      (Klose et al., 2013)
      Comments

      87% of animals homozygous for crbGX24w- and carrying two copies of crb+tfos hatch. The embryonic cuticle defect is completely rescued. The adult flies are fertile and do not show any obvious mutant phenotype.

      (Klose et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      crb+tfos
      Name Synonyms
      Secondary FlyBase IDs
        References (3)