FB2024_03 , released June 25, 2024
Allele: Dmel\Ras85DG12D.UAS.p53sh
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General Information
Symbol
Dmel\Ras85DG12D.UAS.p53sh
Species
D. melanogaster
Name
FlyBase ID
FBal0393052
Feature type
allele
Associated gene
Dmel\Ras85D
Associated Insertion(s)
Carried in Construct
P{UAS-Ras85D(G12D).UAS-p53(shRNA)}
Key Links
Genomic Maps

Transgenic product class
missense_variant
(Sekiya et al., 2023)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Sekiya et al., 2023)
Mutagen
in vitro construct
(Sekiya et al., 2023)
Progenitor genotype
Carried in construct
P{UAS-Ras85D(G12D).UAS-p53(shRNA)}
Cytology
Description

UAS regulatory sequences drive expression of Ras85D containing a G12D amino acid substitution. This change is equivalent to a G12D change in the orthologous human KRAS gene, a pathogenic variant found in most patients with pancreatic ductal adenocarcinoma.

(Sekiya et al., 2023)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ras85D
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
MNV
3R:9,512,256..9,512,257 [-]
Nucleotide change:

GA9512257AT

Reported nucleotide change:

GA?AT

Amino acid change:

G12D | Ras85D-PA

Reported amino acid change:

G12D

Comment:

Analogous mutation in human KRAS implicated in pancreatic ductal carcinoma; mutation carried on in vitro construct.

(Sekiya et al., 2023)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Sekiya et al., 2023)
      KRAS:p.Gly12Asp
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      ClinVar: 12582
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      CycEUAS.Tag:HA.Medsh, MedRNAi.UAS.CycE-HA, Ras85DG12D.UAS.p53sh, Scer\GAL4Ser.PU, p53RNAi.UAS.RasG12D has increased mortality during development phenotype, suppressible | partially by Dsor1LH110

      (Sekiya et al., 2023)

      CycEUAS.Tag:HA.Medsh, MedRNAi.UAS.CycE-HA, Ras85DG12D.UAS.p53sh, Scer\GAL4Ser.PU, p53RNAi.UAS.RasG12D has increased mortality during development phenotype, suppressible | partially by aurBJF03107, Scer\GAL4Ser.PU

      (Sekiya et al., 2023)

      CycEUAS.Tag:HA.Medsh, MedRNAi.UAS.CycE-HA, Ras85DG12D.UAS.p53sh, Scer\GAL4Ser.PU, p53RNAi.UAS.RasG12D has increased mortality during development phenotype, suppressible | partially by Src42Amyri

      (Sekiya et al., 2023)

      CycEUAS.Tag:HA.Medsh, MedRNAi.UAS.CycE-HA, Ras85DG12D.UAS.p53sh, Scer\GAL4Ser.PU, p53RNAi.UAS.RasG12D has increased mortality during development phenotype, suppressible | partially by Rok2

      (Sekiya et al., 2023)

      CycEUAS.Tag:HA.Medsh, MedRNAi.UAS.CycE-HA, Ras85DG12D.UAS.p53sh, Scer\GAL4Ser.PU, p53RNAi.UAS.RasG12D has increased mortality during development phenotype, suppressible | partially by Wee1ES1

      (Sekiya et al., 2023)

      CycEUAS.Tag:HA.Medsh, MedRNAi.UAS.CycE-HA, Ras85DG12D.UAS.p53sh, Scer\GAL4Ser.PU, p53RNAi.UAS.RasG12D has increased mortality during development phenotype, suppressible | partially by aurA87Ac-3

      (Sekiya et al., 2023)

      CycEUAS.Tag:HA.Medsh, MedRNAi.UAS.CycE-HA, Ras85DG12D.UAS.p53sh, Scer\GAL4ptc.PU, p53RNAi.UAS.RasG12D has increased size | larval stage phenotype, suppressible | partially by Dsor1LH110

      (Sekiya et al., 2023)

      CycEUAS.Tag:HA.Medsh, MedRNAi.UAS.CycE-HA, Ras85DG12D.UAS.p53sh, Scer\GAL4ptc.PU, p53RNAi.UAS.RasG12D has increased size | larval stage phenotype, suppressible | partially by aurBJF03107, Scer\GAL4ptc.PU

      (Sekiya et al., 2023)
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ras85DG12D.UAS.p53sh
      Name Synonyms
      Secondary FlyBase IDs
        References (2)