FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS-Ras85D(G12D).UAS-p53(shRNA)}
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General Information
Symbol
P{UAS-Ras85D(G12D).UAS-p53(shRNA)}
FlyBase ID
FBtp0161708
Feature type
transgenic_transposable_element
Component Allele(s)
p53RNAi.UAS.RasG12D
Ras85DG12D.UAS.p53sh
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Ras85DG12D.UAS.p53sh
Transgenic product class
missense_variant
(Sekiya et al., 2023)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ras85D
Description

UAS regulatory sequences drive expression of Ras85D containing a G12D amino acid substitution. This change is equivalent to a G12D change in the orthologous human KRAS gene, a pathogenic variant found in most patients with pancreatic ductal adenocarcinoma.

(Sekiya et al., 2023)
Component allele p53RNAi.UAS.RasG12D
Transgenic product class
RNAi_reagent
(Sekiya et al., 2023)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
p53
Description

UAS regulatory sequences drive expression of an shRNA (TGCTGAAGCAATAACCACCGA) that targets p53.

(Sekiya et al., 2023)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-Ras85D(G12D).UAS-p53(shRNA)}
(FlyBase, 1992-)
UAS-RasG12D, UAS-p53shRNA
(Sekiya et al., 2023)
Secondary FlyBase IDs
    References (2)