FlyBase Allele Report: Dmel\Wee1[ES1]

General Information

Symbol

Dmel\Wee1^ES1

Species

D. melanogaster

Name

FlyBase ID

FBal0117256

Feature type

allele

Associated gene

Dmel\Wee1

Associated Insertion(s)

Carried in Construct

Also Known As

dwee1^ES1, wee^ES1

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Price et al., 2000)

Mutagen

ethyl methanesulfonate

(Price et al., 2000)

Progenitor genotype

Cytology

Description

8bp deletion resulting in a frameshift followed by a stop codon, truncating the protein in kinase domain IV.

(Price et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut

(Willms et al., 2020)

aster | embryonic cycle 12 | maternal effect

(Stumpff et al., 2004)

aster | embryonic cycle 13 | maternal effect

(Stumpff et al., 2004)

centrosome | embryonic cycle 12 | maternal effect

(Stumpff et al., 2005)

centrosome | embryonic cycle 13 | maternal effect

(Stumpff et al., 2005)

condensed nuclear chromosome | embryonic cycle 13 | maternal effect

(Stumpff et al., 2004)

microtubule organizing center | embryonic cycle 12 | maternal effect

(Stumpff et al., 2005)

microtubule organizing center | embryonic cycle 13 | maternal effect

(Stumpff et al., 2005)

mitotic anaphase | embryonic cycle 12 | maternal effect

(Stumpff et al., 2004)

mitotic cell cycle

(Stumpff et al., 2004)

mitotic interphase | maternal effect

(Stumpff et al., 2004)

mitotic telophase | embryonic cycle 12 | maternal effect

(Stumpff et al., 2004)

nucleus | ectopic | embryonic stage 4 | maternal effect

(Stumpff et al., 2005)

nucleus | embryonic cycle 11

(Price et al., 2000)

nucleus | embryonic cycle 12

(Price et al., 2000)

spindle | embryonic cycle 12 | maternal effect

(Stumpff et al., 2005, Stumpff et al., 2004)

spindle | embryonic cycle 13 | maternal effect

(Stumpff et al., 2005)

Detailed Description

Statement

Reference

Wee1^ES1 adult midguts exhibit increased number of dividing cells.

(Willms et al., 2020)

Wee1^ES1 homozygote mutant BrdU-labelled spermatocytes fixed at stage S5/S6 (late G2) do not show any morphological defects in either the nucleolus or the fusome as compared to controls.

(Varadarajan et al., 2016)

Mutant eyes (generated using the "EGUF" system) do not display morphological defects when larvae are raised on media containing 2 mmol caffeine/L and 3 mmol hydroxyurea/L.

(Silva et al., 2006)

Embryos derived from wee^ES1 mothers display monopolar spindles, the inability to form a central spindle in mitosis of cycle 12 and 13, and the failure to fully separate centrosomes during interphase. The following phenotypes are also seen in cycles 12 and 13: 1. spindles with one or two microtubule-organizing centres within the single microtubule network 2. promiscuous interactions between adjacent spindles that initiate in metaphase, anaphase and telophase 3. multipolar spindles that result from centrosome pairs of neighbouring nuclei that interact. Pseudocleavage furrows in wee^ES1 mutants form with normal timing and depths, although the nuclei are displaced and lie beyond the deepest part of the furrows. Additionally, the centrosomes are displaced from the cortex in both interphase and mitosis of cycle 12 in wee^ES1 mutants.

(Stumpff et al., 2005)

Interphase in embryos derived from wee^ES1 hemizygous females is significantly shorter than in control embryos from cycle 11 on. The mutant embryos show no defects in the mitotic spindles during the first cortical mitosis in cycle 11 and during mitosis of cycle 12 (M12), prophase and metaphase figures appear similar to wild type. However, astral microtubules are severely diminished in anaphase and telophase of M12 and the central spindle becomes disorganised. Loss of astral spindles becomes more apparent in cycle M13. DNA condensation defects are seen in embryos derived from wee^ES1 hemizygous females beginning with M13; chromosomes appear less condensed than normal when anaphase begins and are not fully segregated when nuclei exit mitosis. The mutant embryos undergo an extra syncytial division compared to wild-type before becoming arrested with large clumps of abnormal microtubule networks. No evidence of cellularisation is seen, most of the DNA is internalised and the pole cells remain at the posterior end of the embryo, as is typical for syncytial blastoderm stages. Hemizygous mutant larvae have an increased mitotic index and polyploid nuclei in brain neuroblasts.

(Stumpff et al., 2004)

Hemizygous males are fertile. Hemizygous females are viable but completely sterile and show no paternal rescue effect. The females lay abundant eggs of normal appearance that proceed throughout the early syncytial nuclear cycles normally. However, during cycles 11 and 12, nuclei in these embryos fail to separate at the end of mitosis and remain fused. This results in subsequent clumping and fragmentation of nuclei. Embryos derived from wee^DS1/wee^ES1 females never cellularise. wee^ES1/Df(2L)Dwee1-W05 larvae are highly sensitive to hydroxyurea.

(Price et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Wee1^ES1 is a suppressor | partially of increased mortality during development phenotype of CycE^{UAS.Tag:HA.Medsh}, Med^{RNAi.UAS.CycE-HA}, Ras85D^{G12D.UAS.p53sh}, Scer\GAL4^Ser.PU, p53^{RNAi.UAS.RasG12D}

(Sekiya et al., 2023)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Wee1^ES1/wee[+] is a non-enhancer of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

NOT Suppressor of

Statement

Reference

Wee1^ES1 is a non-suppressor of egg chorion phenotype of spn-B^BU

(Abdu et al., 2002)

Wee1^ES1 is a non-suppressor of dorsal appendage phenotype of spn-B^BU

(Abdu et al., 2002)

Wee1^ES1/wee[+] is a non-suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Mutation of lok^p6 in wee^ES1 mutants rescues the anastral spindle and γTURC phenotypes, but fails to rescue the promiscuous spindle interaction and multipolar spindle phenotypes. The displacement of centrosomes from the cortex in interphase of cycle 12 is only partially rescued in wee^ES1, lok^p6 double mutants.

(Stumpff et al., 2005)

Cellularisation and cortical retention of DNA is partially restored in wee^ES1 lok^p6 double mutant embryos and about 60% of the double mutant embryos show evidence of migration changes that normally occur in cellularised embryos (such as the migration of pole cells toward the embryo anterior). The extent of this rescue is variable and none of the double mutant embryos survive to hatching. The distribution and appearance of DNA in these embryos is abnormal.

(Stumpff et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Wee1^ES1/Df(2L)Dwee1-W05 is partially rescued by Wee1^hs.PP

(Price et al., 2000)

Comments

Approximately 50% of embryos derived from wee^ES1/Df(2L)Dwee1-W05 females can be rescued to at least the cellularisation stage (cycle 14) by expression of wee^hs.PP. A wide phenotypic variation in the extent of resue is seen, ranging from mosaic embryos containing both cellularised and syncytial sectors to apparently normal late embryos and first instar larvae that are nonetheless unable to complete development.

(Price et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5833

w¹; Wee1^ES1 cn¹/CyO, P{ActGFP}JMR1

Kyoto

108455

w¹; Wee1^ES1 cn¹/CyO, P{ActGFP}JMR1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Dwee1^ES1

(Heriche et al., 2003, Price et al., 2000)

Dwee^ES1

(Ji et al., 2002)

Dwee^ES

(Abdu et al., 2002)

Wee1^ES1

(Sekiya et al., 2023)

dwee1^ES1

(Stumpff et al., 2005, Stumpff et al., 2004)

wee1^ES1

(Willms et al., 2020, Deneke et al., 2016, Wang and Baker, 2015)

wee^ES1

(Das et al., 2016, Varadarajan et al., 2016, Baumgardt et al., 2014, Silva et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (16)

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