FlyBase Allele Report: Dmel\mle[1]

General Information

Symbol

Dmel\mle¹

Species

D. melanogaster

Name

FlyBase ID

FBal0012332

Feature type

allele

Associated gene

Dmel\mle

Associated Insertion(s)

Carried in Construct

Also Known As

mle

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Kuroda, 2016.3.1)

loss of function allele

(Rastelli and Kuroda, 1998)

Mutagen

spontaneous

(Kernan et al., 1991)

natural population

(Fukunaga, 1980)

Progenitor genotype

Cytology

Description

A single G nucleotide is deleted at position 329 of mle cDNA GB:M74121. This leads to a frameshift, with the first stop codon at amino acid residue 125.

(Kuroda, 2016.3.1)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:5,980,385..5,980,385 [-]

Comment:

A single G nucleotide is deleted at position 329 of mle cDNA GB:M74121. This leads to a frameshift, with the first stop codon at aa 125.

(Kuroda, 2016.3.1)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Aratani et al., 2008)

lethal | larval stage | male

(Chiang and Kurnit, 2003)

lethal | male

(Prabhakaran and Kelley, 2010, Rastelli and Kuroda, 1998, Richter et al., 1996, Kernan et al., 1991, Uenoyama et al., 1982)

lethal | male | heat sensitive

(Rastelli and Kuroda, 1998)

lethal | male | pupal stage

(Chiang and Kurnit, 2003)

lethal | male | recessive

(Okuno et al., 1984, Skripsky and Lucchesi, 1982, Fukunaga, 1980)

lethal - all die before end of larval stage

(Spierer et al., 2008)

lethal - all die before end of larval stage, with mle^ΔMTAD

(Aratani et al., 2008)

lethal - all die before end of larval stage | male | recessive

(Lee et al., 1997)

male sterile

(Rastelli and Kuroda, 1998, Lee et al., 1997)

some die during larval stage | male | recessive

(Lee et al., 1997)

some die during third instar larval stage

(Spierer et al., 2008)

some die during third instar larval stage, with mle^ΔMTAD

(Aratani et al., 2008)

Phenotype Manifest In

abdomen & macrochaeta

(Lee et al., 1997)

chromosome

(Spierer et al., 2008)

spermatozoon | heat sensitive

(Rastelli and Kuroda, 1998)

wing

(Lee et al., 1997)

Detailed Description

Statement

Reference

mle¹ mutants display strong X chromosome condensation.

(Spierer et al., 2008)

Male lethal at the late third instar to early pupal stage. Homozygous female larvae are viable.

(Chiang and Kurnit, 2003)

mle¹/mle⁶ males are temperature sensitive. At 18^oC no significant lethality or sterility is observed. At 25^oC males are viable but sterile, sperm are fully formed but non-motile. At 29^oC no males reach adulthood, they die during late third instar and pupal stages. Homozygous males shows morphological signs that spermatogenesis has advanced to post-meiotic stages.

(Rastelli and Kuroda, 1998)

mle^GET allows male to survive to mid to late larval stages, the same as males not carrying mle^GET. A few male that do survive to adulthood are sterile and hold out their wings and have disorganised abdominal bristles.

(Lee et al., 1997)

Homozygous males show abnormal dosage compensation as determined by tritiated uridine labeling of polytene chromosomes.

(Okuno et al., 1984)

male-lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mle¹ has lethal | third instar larval stage phenotype, suppressible by Su(var)3-7^FL.hs.Tag:HA

(Spierer et al., 2008)

Enhancer of

Statement

Reference

mle¹/mle[+] is an enhancer | maternal effect of partially lethal - majority die | dominant | female phenotype of sc^sisB-1, sisA[+]/sisA¹

(Gladstein et al., 2010)

msl-1¹, msl-1[+], msl-2[+], msl-2¹, mle¹, mle[+] is an enhancer of lethal | male phenotype of Trl⁶²/Trl^13C

(Greenberg et al., 2004)

NOT Enhancer of

Statement

Reference

mle¹/mle[+] is a non-enhancer of lethal | male phenotype of Trl^2.3/Trl^13C

(Greenberg et al., 2004)

mle¹/mle[+] is a non-enhancer of partially lethal | female phenotype of Trl^2.3/Trl^13C

(Greenberg et al., 2004)

msl-1¹, msl-1[+], msl-2[+], msl-2¹, mle¹, mle[+] is a non-enhancer of partially lethal | female phenotype of Trl⁶²/Trl^13C

(Greenberg et al., 2004)

Suppressor of

Statement

Reference

mle¹/mle[+] is a suppressor | partially of lethal | male phenotype of ocm^L1658N/ocm^G1646E

(Lim and Kelley, 2013)

NOT Suppressor of

Statement

Reference

msl-1¹, msl-1[+], msl-2[+], mle¹, msl-2¹, mle[+] is a non-suppressor of partially lethal | female phenotype of Trl^2.3/Trl^13C

(Greenberg et al., 2004)

mle¹/mle[+] is a non-suppressor of partially lethal | female phenotype of Trl⁶²/Trl^13C

(Greenberg et al., 2004)

Other

Statement

Reference

Sxl^f1, mle¹ has abnormal sex-determination | female phenotype

(Skripsky and Lucchesi, 1982)

Sxl^f1, mle¹ has visible | female phenotype

(Skripsky and Lucchesi, 1982)

Sxl^f1, mle¹ has abnormal sex-determination | dominant | female phenotype

(Skripsky and Lucchesi, 1982)

Sxl^f1, mle¹ has visible | dominant | female phenotype

(Skripsky and Lucchesi, 1982)

Phenotype Manifest In

Suppressed by

Statement

Reference

mle¹ has FlyBase_internalproperty type:chromosome:chromosome phenotype, suppressible by Su(var)3-7^FL.hs.Tag:HA

(Spierer et al., 2008)

Suppressor of

Statement

Reference

mle¹/mle¹ is a suppressor of polytene chromosome | male phenotype of Su(var)3-7¹⁴

(Spierer et al., 2005)

mle¹ is a suppressor of X chromosome | male phenotype of Iswi²

(Corona et al., 2002)

mle¹ is a suppressor of ovary phenotype of ovo^D1rv23

(Oliver and Pauli, 1998)

mle¹⁴/mle¹ is a suppressor of ovary phenotype of ovo^D1rv23

(Oliver and Pauli, 1998)

mle⁵/mle¹ is a suppressor of ovary phenotype of ovo^D1rv23

(Oliver and Pauli, 1998)

mle¹ is a suppressor of ovary phenotype of ovo^D1rv22

(Oliver and Pauli, 1998)

mle¹⁴/mle¹ is a suppressor of ovary phenotype of ovo^D1rv22

(Oliver and Pauli, 1998)

mle⁵/mle¹ is a suppressor of germline cell phenotype of ovo^D1rv22/ovo^D1rv23

(Oliver et al., 1993)

Other

Statement

Reference

Sxl^M1, mle¹, ovo^D1rv22 has ovary phenotype

(Oliver and Pauli, 1998)

Sxl^M1, mle⁵/mle¹, ovo^D1rv23 has ovary phenotype

(Oliver and Pauli, 1998)

Sxl^M1, mle¹, ovo^D1rv23 has ovary phenotype

(Oliver and Pauli, 1998)

Sxl^f1, mle¹ has prothoracic metatarsus | female phenotype

(Skripsky and Lucchesi, 1982, Uenoyama et al., 1982)

Sxl^f1, mle¹ has abdominal sternite 6 | female phenotype

(Uenoyama et al., 1982)

Sxl^f1, mle¹ has abdominal tergite 6 | female phenotype

(Uenoyama et al., 1982)

Sxl^f1, mle¹ has abdominal tergite 5 | female phenotype

(Uenoyama et al., 1982)

Sxl^f1, mle¹ has oviprovector phenotype

(Uenoyama et al., 1982)

Additional Comments

Genetic Interactions

Statement

Reference

The viability of Sxl^fP7B0/+ females is not affected if they also carry one maternally-derived copy of mle¹.

One maternally-derived copy of mle¹ significantly reduces the viability of sc^sisB-1 sisA¹/+ females so that they show almost complete lethality. One paternally-derived copy of mle¹ significantly reduces the viability of sc^sisB-1 sisA¹/+ females, although this zygotic effect is generally weaker than the maternal one.

(Gladstein et al., 2010)

The presence of Su(var)3-7^{FL.hs.T:Ivir\HA1} suppresses the mle¹ phenotype resulting in almost normal male X chromosome morphology.

(Spierer et al., 2008)

Homozygosity for mle¹ suppresses the bloated X chromosome phenotype seen in polytene chromosome squashes from male homozygous Su(var)3-7¹⁴ third instar larvae.

(Spierer et al., 2005)

mle¹ completely suppresses the morphological defects of the X chromosome that are seen in salivary gland polytene chromosome preparations from Iswi² males.

(Corona et al., 2002)

Ovaries of homozygous ovo^D1rv23 females contain germline chambers in less than 20% of cases. This phenotype is partially suppressed if the females are also carrying mle¹/mle⁵ or mle¹/mle¹⁴. Ovaries of ovo^D1rv23/ovo^D1rv22 females contain germline chambers in less than 20% of cases. This phenotype is partially suppressed if the females are also carrying mle¹/mle¹. Ovaries of homozygous ovo^D1rv22 females contain germline chambers in less than 20% of cases. This phenotype is partially suppressed if the females are also carrying mle¹/mle¹ or mle¹/mle¹⁴. ovo^D1rv22/ovo^D1rv22; mle¹/mle¹ ovaries most often contain germ cells of male morphology rather than female morphology. This phenotype is partially suppressed by Sxl^M1; ovaries more often contain germ cells of female morphology rather than male morphology. ovo^D1rv23/ovo^D1rv23; mle¹/mle⁵ ovaries most often contain germ cells of male morphology rather than female morphology. This phenotype is partially suppressed by Sxl^M1; ovaries more often contain germ cells of female morphology rather than male morphology.

(Oliver and Pauli, 1998)

The survival of 2X ovo^D1rv23/ovo^D1rv22 germline cells is enhanced in an mle¹/mle⁵ background. Surviving cells usually form small ovarian tumours.

(Oliver et al., 1993)

Some females homozygous for mle¹ and heterozygous for Sxl^f1 have sex combs on the basitarsi of their forelegs. This phenotype occurs with a higher frequency in females with homozygous mle¹ mothers.

(Skripsky and Lucchesi, 1982)

Sxl^f1/+ chromosomal females also homozygous or heterozygous mle¹ show variable transformation of sexually dimorphic structures to male characteristics. The frequency of transformation is not high, is higher in females homozygous (rather than heterozygous) for mle¹, and expression is incomplete. The frequency of transformation is higher in female flies with mothers homozygous mle¹. Sex combs are only seen in Sxl^f1/+ females homozygous for mle¹.

(Uenoyama et al., 1982)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mle¹ is rescued by mle^+t10.2

(Aratani et al., 2008)

mle¹ is rescued by mle^+t10.5

(Lee et al., 1997)

mle¹ is rescued by mle^ATG.Tag:HA

(Richter et al., 1996)

mle¹ is rescued by mle^AAA.Tag:HA

(Richter et al., 1996)

mle¹ is rescued by mle^+t10.5

(Richter et al., 1996)

mle¹ is rescued by mle^hs.Tag:HA

(Richter et al., 1996)

mle¹ is rescued by mle^DQIH.Tag:HA

(Richter et al., 1996)

mle¹ is rescued by mle^+20.Tag:HA

(Richter et al., 1996)

mle¹ is rescued by mle^STOP.Tag:HA

(Richter et al., 1996)

mle¹ is rescued by mle^hs.PR

(Richter et al., 1996)

mle¹ is rescued by mle^ts.Tag:HA

(Richter et al., 1996)

mle¹ is rescued by mle^GNT.Tag:HA

(Richter et al., 1996)

Partially rescued by

mle¹ is partially rescued by mle^Δgly

(Prabhakaran and Kelley, 2010)

Not rescued by

mle¹ is not rescued by mle^{ΔRB1.Hsp83.Tag:FLAG}

(Morra et al., 2011)

mle¹ is not rescued by mle^{ΔRB2.Hsp83.Tag:FLAG}

(Morra et al., 2011)

mle¹ is not rescued by mle^{Δ1,2.Hsp83.Tag:FLAG}

(Morra et al., 2011)

mle¹ is not rescued by mle^{ΔG.Hsp83.Tag:FLAG}

(Morra et al., 2011)

mle¹ is not rescued by mle^ΔMTAD

(Aratani et al., 2008)

mle¹ is not rescued by mle^GET

(Lee et al., 1997)

Comments

The lethality of mle¹ homozygous males is rescued by mle^Δgly, but the rescued males are sterile.

(Prabhakaran and Kelley, 2010)

mle^+t10.2 fails to rescue the lethality of mle¹. The mutant die during late larval stages.

mle^+t10.2 rescues the lethality of mle¹.

(Aratani et al., 2008)

The lethality of mle¹ males is rescued by mle^hs.PR, mle^{hs.T:Ivir\HA1}, mle^+t10.5, mle^{ATG.T:Ivir\HA1}, mle^{+20.T:Ivir\HA1}, mle^{STOP.T:Ivir\HA1}, mle^{GNT.T:Ivir\HA1}, mle^{DQIH.T:Ivir\HA1}, mle^{AAA.T:Ivir\HA1} or mle^{ts.T:Ivir\HA1}.

(Richter et al., 1996)

Mutant phenotype can be rescued by P element mediated transformation of a wild type mle gene copy.

(Kernan et al., 1991)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4235

y¹ w¹ N^spl-1/Dp(1;Y)Bar^S; mle¹/SM1

Kyoto

107647

y¹ w¹ N^spl-1/Dp(1;Y)Bar^S; mle¹/SM1

Notes on Origin

Discoverer

Oshima, 1971.

Isolated from: Kofu, Japan population, 1971. Separable from: mfs(2)350¹. Recovered as the "350" second chromosome which carries both mfs(2)350¹ and mle¹.

(Fukunaga, 1980)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Name Synonyms

Secondary FlyBase IDs

References (41)

Report Sections

Open Close