FlyBase Allele Report: Dmel\ovo[D1rv23]

Insertion of a 7660bp Stalker2 element, at position 4292 within ovo, in alternative exon 2b. There is duplication of a 6bp sequence at the insertion site. The insertion disrupts the exon 2b reading frame after residue 1082, resulting in a stop codon 4 residues downstream.

(Salles et al., 2002)

Insertion of 5.8kb DNA, which is probably an almost full-length HMS-Beagle element.

(Garfinkel et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:5,067,673..5,067,673 [-]

(Salles et al., 2002, Garfinkel et al., 1992)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Andrews et al., 2000, Oliver and Pauli, 1998, Mevel-Ninio et al., 1991, Oliver et al., 1990)

male fertile

(Oliver et al., 1987)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant germaria are agametic. In 2 day old mutant females, many germaria still contain one to five cap cells, although some germaria completely lack cap cells.

(Song et al., 2007)

Homozygous ovaries have an agametic phenotype.

(Salles et al., 2002)

Ovaries of homozygous females are devoid of germ cells.

(Andrews et al., 2000)

The ovaries of ovo^D1rv23 females are primarily agametic.

(Waterbury et al., 2000)

XX tra¹/tra⁴ pseudomales that are also homozygous for ovo^D1rv23 show an increase in the frequency of atrophic gonads compared to XX tra¹/tra⁴ pseudomales. Non-oogenic gonads, with spectrosomes and multibranched fusomes are also seen (at the same frequency as XX tra¹/tra⁴ pseudomales). Oogenic gonads are not seen in XX tra¹/tra⁴ pseudomales carrying otu^hs.PN that are also homozygous for ovo^D1rv23. In each case the frequency of non-oogenic gonads is increased compared to XX tra¹/tra⁴ pseudomales also homozygous for ovo^D1rv23.

(Hinson and Nagoshi, 1999)

In homozygous mutant germaria, very few ovary lobes contain any egg chambers. Few germ cells are found, those germ cells that are found usually have no spectrosome or fusome material. In those rare cases that do, fusomes are small and poorly branched indicating aberrant and aborted differentiation early in gametogenesis. Those rare egg chambers that are found are also small and have no nurse cells. ovo^e7E/ovo^D1rv23 mutants have egg chambers arrested in pre-vitellogenic stages.

(Hinson et al., 1999)

Ovaries of homozygous females contain germline chambers in less than 20% of cases. Ovaries of ovo^D1rv23/ovo^D1rv22 females contain germline chambers in less than 20% of cases.

(Oliver and Pauli, 1998)

Homozygous female embryos contain the normal number of germ cells. Most homozygous female first instar larvae have the wild-type number of germ cells, although by the end of the third larval instar many homozygous female larvae have a reduced number or no germ cells.

(Staab and Steinmann-Zwicky, 1996)

XX females have agametic ovaries. ovo^D1rv23/Y pseudofemales (carrying tra^Hsp83.PS) have ovaries that contain large numbers of tumorous egg cysts. XX homozygotes transformed into pseudomales with tra¹/tra⁴ show pseudotestes characteristic of group 1, either empty or containing only undifferentiated germ cells.

(Nagoshi et al., 1995)

In contrast to a previous study (FBrf0046291) no significant reduction in the number of germ cells in female homozygous embryos was detected. Any early reduction in germ cell numbers is either transient or inconsistent. Many larval homozygous ovaries have near wild type morphology. Many larval ovaries of the agametic heteroallelic combination ovo^D1rv23/ovo^D1rv22 also have near wild type morphology. By late pupae homozygous gonads fail to produce egg chambers and germ cells that are present fail to differentiate.

(Rodesch et al., 1995)

Increased amounts of cuticular hydrocarbons.

(Wicker and Jallon, 1995)

Ovaries are atrophic and have no germ cells. Rare, escaping germ cells are usually found as single cells or small clusters in a single ovariole, though they sometimes form cysts. ovo^-,tra^hs.PB XY ovaries have abundant germ cells. The germ cells of XO ovo^- males have the crystals characteristic of XO males and are fully populated with male germ cells of all stages.

(Oliver et al., 1994)

Phenotypic category: svb⁺ ovo^-.

(Garfinkel et al., 1992)

The germ cells of homozygous females die. Homozygous females also heterozygous for fl(2)d¹ (at 29^oC) or fl(2)d² (at 25^oC) have small ovaries with a few ovarioles containing mainly multicellular cysts and cysts with 16 cells resembling young egg chambers.

(Granadino et al., 1992)

Lack of egg chamber formation.

(Mevel-Ninio et al., 1991)

Germ cell death occurs during early gastrulation in female homozygotes. This phenotype is suppressed in females also homozygous for Sxl^f1. The soma is wild-type.

(Oliver et al., 1990)

Gene activity is not detectable. Viable. Male germ line is fertile, female germ line has no vitellogenic egg chambers.

(Oliver et al., 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

ovo^D1rv23 has ovary phenotype, suppressible by otu^hs.PN

(Hinson et al., 1999)

ovo^D1rv23 has germline cell | female phenotype, suppressible by otu^hs.PN

(Hinson et al., 1999)

ovo^D1rv23 has egg chamber phenotype, suppressible by otu^hs.PN

(Hinson et al., 1999)

ovo^D1rv23 has egg phenotype, suppressible by otu^hs.PN

(Hinson et al., 1999)

ovo^D1rv23 has ovary phenotype, suppressible by mle¹

(Oliver and Pauli, 1998)

ovo^D1rv23 has ovary phenotype, suppressible by mle¹⁴/mle¹

(Oliver and Pauli, 1998)

ovo^D1rv23 has ovary phenotype, suppressible by mle⁵/mle¹

(Oliver and Pauli, 1998)

ovo^D1rv22/ovo^D1rv23 has germline cell phenotype, suppressible by mle⁵/mle¹

(Oliver et al., 1993)

NOT suppressed by

Statement

Reference

ovo^D1rv23 has ovary phenotype, non-suppressible by Sxl^M4

(Oliver and Pauli, 1998)

ovo^D1rv23 has ovary phenotype, non-suppressible by Sxl^M1

(Oliver and Pauli, 1998)

ovo^D1rv23 has ovary phenotype, non-suppressible by msl-2¹³⁸

(Oliver and Pauli, 1998)

ovo^D1rv23 has ovary phenotype, non-suppressible by msl-2¹³⁸/msl-2¹

(Oliver and Pauli, 1998)

Other

Statement

Reference

Sxl^M1, mle⁵/mle¹, ovo^D1rv23 has ovary phenotype

(Oliver and Pauli, 1998)

Sxl^M1, mle¹, ovo^D1rv23 has ovary phenotype

(Oliver and Pauli, 1998)

mle¹⁴/mle⁹, ovo^D1rv23 has ovary phenotype

(Oliver and Pauli, 1998)

Sxl^M1, mle⁵, ovo^D1rv23 has ovary phenotype

(Oliver and Pauli, 1998)

Additional Comments

Genetic Interactions

Statement

Reference

ovo^D1rv23 have no adverse effect on the development of the germline in XY tra^F.Hsp83 pseudofemales; the pseudo-ovaries are filled with a multitude of undifferentiated germ cells.

(Waterbury et al., 2000)

Addition of otu^hs.PN modestly improves the ovo^D1rv23 phenotype. In mutant females homozygous for ovo^D1rv23 and with one copy of otu^hs.PN, the number of ovary lobes seen with at least one egg chamber increases. However a small number of egg chambers per ovary are seen, with almost all of these displaying a tumorous phenotype, and those without this phenotype have a mixture of tumorous and nurse-like cells. In addition there is some improvement in the degree of oogenic differentiation as the frequency of germ cell clusters with hts positive ring canals or nurse-like cells increases. However chambers remain small and do not progress beyond stage 4. The addition of more than one copy of otu^hs.PN have no extra effect. The addition of otu^hs.PN to ovo^D1rv23/ovo^M1, ovo^D1rv23/ovo^M2 or ovo^D1rv23/ovo^e8F mothers leads to an increase in the number of eggs produced, though in no case is hatching observed.

(Hinson et al., 1999)

The ovary phenotype of homozygous ovo^D1rv23 females is partially suppressed if they are also carrying mle¹/mle⁵ or mle¹/mle¹⁴. The ovary phenotype is of ovo^D1rv23/ovo^D1rv22 females partially suppressed if they are also carrying mle¹/mle¹. The phenotype is not affected if the females are also carrying Sxl^M4 or Sxl^M1. The phenotype is not affected if the females are also carrying msl-2¹³⁸/msl-2¹³⁸ or msl-2¹³⁸/msl-2¹. ovo^D1rv23/ovo^D1rv22; mle⁹/mle¹⁴ ovaries are phenotypically mosaic, containing germ cells of both male and female morphology. The mosaicism can be at the level of whole ovaries or ovarioles. ovo^D1rv23/ovo^D1rv23; mle¹/mle⁵ ovaries most often contain germ cells of male morphology rather than female morphology. This phenotype is partially suppressed by Sxl^M1; ovaries more often contain germ cells of female morphology rather than male morphology.

(Oliver and Pauli, 1998)

The survival of 2X ovo^D1rv23/ovo^D1rv22 germline cells is enhanced in an mle¹/mle⁵ background. Surviving cells usually form small ovarian tumours. Sxl^M1 partially rescues the morphology of the otu mutant ovarian tumour cells.

(Oliver et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ovo^e8F

(Oliver and Pauli, 1998)

ovo^M2

(Oliver and Pauli, 1998)

ovo^M1

(Oliver and Pauli, 1998)

ovo^D1rv22

(Oliver and Pauli, 1998)

ovo^e7E

(Oliver and Pauli, 1998)

ovo^e8K

(Oliver and Pauli, 1998)

Rescued by

ovo^D1rv23 is rescued by ovo^+2b

(Salles et al., 2002)

ovo^D1rv23 is rescued by ovo^Δap

(Andrews et al., 2000)

ovo^D1rv23 is rescued by ovo^amk

(Andrews et al., 2000)

ovo^D1rv23 is rescued by ovo^Δa2

(Andrews et al., 2000)

ovo^D1rv23 is rescued by ovo^amv

(Andrews et al., 2000)

Partially rescued by

ovo^D1rv23 is partially rescued by ovo^-2b

(Salles et al., 2002)

Comments

ovo^D1rv23 females carrying two copies of ovo^-2b have ovaries with a variety of defects. Egg chambers in which follicle cells have failed to migrate and degenerating ovarioles are seen. Egg chambers develop to maturity in rare cases, giving rise to short, abnormal eggs which have ventralised eggshells and fused dorsal appendages.

(Salles et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38443

y¹ w^* ovo^D1rv23 v²⁴/FM6, w^*

Notes on Origin

Discoverer

Comments

Mutation greatly reduces the ability of Sxl^cF1.otu to induce female Sxl⁺ RNA splicing in the male germline: number of autoregulatory cysts in testes is much reduced.

(Hager and Cline, 1997)

Severe ovo allele.

(Rodesch et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

ovo^D1rS1

(Song et al., 2007, Hinson and Nagoshi, 1999, Hager and Cline, 1997, Staab and Steinmann-Zwicky, 1996, Nagoshi et al., 1995, Rodesch et al., 1995, Oliver et al., 1994, Oliver et al., 1993, Garfinkel et al., 1992, Granadino et al., 1992, Mevel-Ninio et al., 1991, Oliver et al., 1990, Oliver et al., 1987)

ovo^D1rs1

(Waterbury et al., 2000, Wicker and Jallon, 1995)

ovo^D1rv23

ovoD1^rv23

(Sahut-Barnola and Pauli, 1999)

Name Synonyms

Secondary FlyBase IDs

References (26)

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