FlyBase Allele Report: Dmel\otu[hs.PN]

General Information

Symbol

Dmel\otu^hs.PN

Species

D. melanogaster

Name

heat shock construct of Nagoshi

FlyBase ID

FBal0044237

Feature type

allele

Associated gene

Dmel\otu

Associated Insertion(s)

Carried in Construct

P{hs-otu.N}

Also Known As

hs-otu

Key Links

Transgenic product class

genomic_DNA

(Nagoshi et al., 1995)

wild_type

(Nagoshi et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Nagoshi et al., 1995)

wild_type

(Nagoshi et al., 1995)

Mutagen

in vitro construct

(Nagoshi et al., 1995, Rodesch et al., 1995)

Progenitor genotype

Carried in construct

P{hs-otu.N}

Cytology

Description

Genomic otu fragment fused to the Hsp70 promoter.

(Nagoshi et al., 1995)

4kb HpaI otu genomic fragment (starting 64 nucleotides downstream of the transcription start site and ending 139 nucleotides downstream of translation stop codon) is inserted between an Hsp70 promoter and polyadenylation sequences of αTub84B.

(Rodesch et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

otu

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

wild-type

(Rodesch et al., 1995)

Phenotype Manifest In

germline cell

(Nagoshi et al., 1995)

Detailed Description

Statement

Reference

Under heat shock conditions this construct suppresses the ovarian defects resulting from otu mutations. An increase in otu activity in XX otu^hs.PN, tra¹/tra⁴ pseudomales leads to elongated pseudotestes categorized as having a group 3 phenotype.

(Nagoshi et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

otu^hs.PN, tra¹ has abnormal sex-determination | recessive phenotype

(Hinson and Nagoshi, 1999)

otu^hs.PN, tra⁴ has abnormal sex-determination | recessive phenotype

(Hinson and Nagoshi, 1999)

Phenotype Manifest In

Suppressor of

Statement

Reference

otu^hs.PN is a suppressor of egg chamber phenotype of ovo^D1rv23

(Hinson et al., 1999)

otu^hs.PN is a suppressor of egg phenotype of ovo^D1rv23

(Hinson et al., 1999)

otu^hs.PN is a suppressor of egg chamber phenotype of ovo^e8F

(Hinson et al., 1999)

otu^hs.PN is a suppressor of egg phenotype of ovo^e8F

(Hinson et al., 1999)

otu^hs.PN is a suppressor of ovary phenotype of ovo^e8F

(Hinson et al., 1999)

otu^hs.PN is a suppressor of ovary phenotype of ovo^D1rv23

(Hinson et al., 1999)

otu^hs.PN is a suppressor of germline cell | female phenotype of ovo^D1rv23

(Hinson et al., 1999)

otu^hs.PN is a suppressor of egg chamber phenotype of ovo^M1

(Hinson et al., 1999)

otu^hs.PN is a suppressor of egg phenotype of ovo^M1

(Hinson et al., 1999)

otu^hs.PN is a suppressor of ovary phenotype of ovo^M1

(Hinson et al., 1999)

otu^hs.PN is a suppressor of egg chamber phenotype of ovo^M2

(Hinson et al., 1999)

otu^hs.PN is a suppressor of egg phenotype of ovo^M2

(Hinson et al., 1999)

otu^hs.PN is a suppressor of ovary phenotype of ovo^M2

(Hinson et al., 1999)

Other

Statement

Reference

otu^hs.PN, tra¹ has gonad phenotype

(Hinson and Nagoshi, 1999)

otu^hs.PN, tra⁴ has gonad phenotype

(Hinson and Nagoshi, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

otu^hs.PN does not significantly alter the pseudomale phenotype in tra2 flies.

(Hinson and Nagoshi, 2002)

XX tra¹/tra⁴ pseudomales that are also carrying otu^hs.PN and raised at 20-25^oC are 2 to 3 times longer than normal. Expression of otu^hs.PN partially rescues XX tra¹/tra⁴ pseudomales; 88% of the gonads are 'feminised' and show a developmental progression of gametogenic stages, which can be divided into three sections corresponding to germarial regions 1-3 in normal oogenesis. Section I includes the most apical portion of the pseudomale testis and contains gametogenic stages characteristic of germarial region 1 in ovaries. A group of cells containing spectrosomes is localised at the distal tip. Clusters of cells connected by branched fusomes are located immediately adjacent to the distal cells. The fusomes have disappeared in section II and differentiation of the ring canals continues. In section II, the pseudomale germ cells have differentiated to postgermarial stages. The germ cell clusters contain thick ring canals and the nuclei are large.

(Hinson and Nagoshi, 1999)

Addition of otu^hs.PN modestly improves the ovo^D1rv23 phenotype. In mutant females homozygous for ovo^D1rv23 and with one copy of otu^hs.PN, the number of ovary lobes seen with at least one egg chamber increases. However a small number of egg chambers per ovary are seen, with almost all of these displaying a tumorous phenotype, and those without this phenotype have a mixture of tumorous and nurse-like cells. In addition there is some improvement in the degree of oogenic differentiation as the frequency of germ cell clusters with hts positive ring canals or nurse-like cells increases. However chambers remain small and do not progress beyond stage 4. The addition of more than one copy of otu^hs.PN have no extra effect. The addition of otu^hs.PN to ovo^D1rv23/ovo^M1, ovo^D1rv23/ovo^M2 or ovo^D1rv23/ovo^e8F mothers leads to an increase in the number of eggs produced, though in no case is hatching observed.

(Hinson et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

otu^hs.PN rescues otu¹⁴

(Rodesch et al., 1995)

Partially rescues

otu^hs.PN partially rescues otu^unspecified

(Hinson et al., 1999)

otu^hs.PN partially rescues Df(1)otu-PΔ1/otu¹⁰

(Rodesch et al., 1995)

otu^hs.PN partially rescues otu¹

(Rodesch et al., 1995)

otu^hs.PN partially rescues otu¹⁰

(Rodesch et al., 1995)

otu^hs.PN partially rescues otu¹³

(Rodesch et al., 1995)

otu^hs.PN partially rescues otu³

(Rodesch et al., 1995)

otu^hs.PN partially rescues otu^PΔ3

(Rodesch et al., 1995)

Comments

One copy can suppress the otu null phenotype at room temperature, allowing egg chamber maturation to late oogenic stages, but typically does not support normal fertility or fecundity.

(Hinson et al., 1999)

Complete rescue of both fertility and fecundity were obtained with only the weakest otu allele, otu¹⁴. More severe alleles that cause agametic (Df(1)otu-PΔ1 and otu¹⁰) or tumorous (otu¹, otu³, otu¹³ and otu^PΔ3) phenotypes show substantial morphological suppression but were more variable in their fertility. The same ovarian phenotype is produced with two copies of P{hs-otu.N}.

(Rodesch et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Partial suppression of the fertility defect does not result from insufficient otu activity.

(Rodesch et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

otu^hs.PN

Name Synonyms

heat shock construct of Nagoshi

(Nagoshi et al., 1995)

Secondary FlyBase IDs

References (5)

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