FlyBase Allele Report: Dmel\otu[10]

General Information

Symbol

Dmel\otu¹⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0013357

Feature type

allele

Associated gene

Dmel\otu

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Oliver et al., 1993)

Mutagen

ethyl methanesulfonate

(Rodesch et al., 1995, Storto and King, 1987, King et al., 1986)

Progenitor genotype

Cytology

Description

The 3' splice site of intron 5 has been eliminated.

(Glenn and Searles, 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,487,715..8,487,715 [-]

Comment:

The 3' splice site of intron 5 has been eliminated. The mutation in the splice site has been arbitrarily mapped by a FlyBase curator to the first base of the splice acceptor site.

(Glenn and Searles, 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | female

(Tirronen et al., 1993)

female sterile

(Rodesch et al., 1995, Sass et al., 1995, Bae et al., 1994)

female sterile | recessive

(King et al., 1986)

Phenotype Manifest In

egg chamber

(Rodesch et al., 1995)

germline cell

(Nagoshi et al., 1995)

ovariole

(Pauli et al., 1993, Storto and King, 1987, King et al., 1986)

ovary

(Nagoshi et al., 1995, Bae et al., 1994)

Detailed Description

Statement

Reference

Homozygous females lack egg chambers. XY pseudofemales contain gonads that are similarly agametic. 90% of pseudoovaries lack egg cysts with the rest containing fewer than five chambers. Pseudofemales carrying a duplication of the otu gene have more than 90% ovaries with tumorous egg cysts.

(Nagoshi et al., 1995)

Ovaries are devoid of egg chambers. Numbers and morphology of embryonic germ cells during stages when pole cells invaginate and form the embryonic gonads are no different to wild type. Larval ovaries are morphologically indistinguishable from wild type, gross aberrations have not yet occurred. By late pupae gonads fail to produce egg chambers and germ cells that are present fail to differentiate. otu¹⁰ homozygotes and otu¹/Df(1)otu-PΔ1 transheterozygotes carrying otu^98.hs have relatively large ovaries containing many tumorous egg chambers.

(Rodesch et al., 1995)

Chamberless ovaries. Transheterozygotes with otu¹³ produce ovaries containing predominantly tumorous egg cysts.

(Bae et al., 1994)

Spermatogenesis normal but mating behaviour fails because wild type females refuse to react to the courtship attempts of mutant males. Effect is not absolute, and depends on allele (there is a strong correlation between male sterility and severity of impairment in the female phenotype) and varies between affecting 6.7% (otu¹⁴) and 86.6% (otu¹²) of mutant males.

(Tirronen et al., 1993)

Homozygous ovarioles have quiescent oogonia.

(Storto and King, 1987)

Quiescent allele; homozygous and hemizygous ovarioles contain vitellaria that lack developing follicles.

(King et al., 1986)

quiescent; ovarioles lack germ cells

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

otu¹⁰ has female sterile phenotype, non-suppressible by Sxl^M1

(Bae et al., 1994)

Phenotype Manifest In

Suppressed by

Statement

Reference

otu¹⁰ has ovary phenotype, suppressible by Sxl^M1

(Bae et al., 1994)

Additional Comments

Genetic Interactions

Statement

Reference

otu¹⁰ causes severe germ cell loss in XY tra^F.Hsp83 pseudofemales and 4 out of 5 pseudo-ovaries are completely agametic.

(Waterbury et al., 2000)

Sxl^M1 incompletely suppresses the otu¹⁰/otu¹³ mutant phenotype, ovaries are at a more advanced stage of oogenesis but females are still completely sterile.

(Bae et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

otu¹⁰ is partially rescued by otu⁴²³

(Glenn and Searles, 2001)

otu¹⁰ is partially rescued by otu⁶²⁷

(Glenn and Searles, 2001)

Df(1)otu-PΔ1/otu¹⁰ is partially rescued by otu^hs.PN

(Rodesch et al., 1995)

otu¹⁰ is partially rescued by otu^hs.PN

(Rodesch et al., 1995)

otu¹⁰ is partially rescued by otu^98.hs

(Rodesch et al., 1995)

Not rescued by

otu¹⁰ is not rescued by otu³³⁸

(Glenn and Searles, 2001)

otu¹⁰ is not rescued by otu²⁵⁰

(Glenn and Searles, 2001)

otu¹⁰ is not rescued by otu³⁸⁰

(Glenn and Searles, 2001)

Comments

One or two copies of P{hs-otu.N} cause substantial morphological suppression but only partial suppression of sterility. otu¹⁰ homozygotes and otu¹/Df(1)otu-PΔ1 transheterozygotes carrying otu^98.hs have relatively large ovaries containing many tumorous egg chambers.

(Rodesch et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Mohler.

Comments

Quiescent (QUI) class allele. The ovarian phenotype of flies transheterozygous for otu¹⁰ and a number of other otu mutations has been analysed.

(Storto and King, 1987)

Shows a dominant interaction with ovo^D2 and ovo^D3 : more extreme mutant phenotype as assayed by reduction in number of egg chambers at stage 10 or further developed. There is no apparent correlation with strength of otu allele and its interaction with the ovo alleles.

(Pauli et al., 1993)

Severe mutation that eliminates the function of both the 98kD and 104kD isoforms.

(Bae et al., 1994)

QUI class of otu allele. P{otu-98} fails to restore detectable levels of fertility, P{otu-104} completely restores fertility.

(Sass et al., 1995)

Severe otu allele.

(Rodesch et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

12-4474

(Mohler and Carroll, 1984)

fs(1)12-4474

fs12-4474

(King et al., 1986)

otu¹⁰

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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