FlyBase Allele Report: Dmel\otu[14]

General Information

Symbol

Dmel\otu¹⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0013361

Feature type

allele

Associated gene

Dmel\otu

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Pauli et al., 1993)

Mutagen

ethyl methanesulfonate

(Storto and King, 1987, King et al., 1986)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?698term.

(Glenn and Searles, 2001)

Nucleotide substitution: C4120T.

(Tirronen et al., 1995, Steinhauer and Kalfayan, 1992)

Amino acid replacement: ?656term.

(Tirronen et al., 1995)

Polypeptide is truncated for the last 156 amino acids (Steinhauer et al., Mol. Cell Biol. 9:5726-5732 .

(Pauli et al., 1993)

Amino acid replacement: ?622term.

(Steinhauer and Kalfayan, 1992)

Amino acid numbering is with respect to the 98kD isoform.

(Steinhauer and Kalfayan, 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,486,042..8,486,042 [-]

Nucleotide change:

C8486042T

Reported nucleotide change:

C4120T

Amino acid change:

Reported amino acid change:

?656term

(Tirronen et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | female

(Tirronen et al., 1993)

female sterile

(Sass et al., 1995, Bae et al., 1994)

female sterile | recessive

(King et al., 1986)

Phenotype Manifest In

egg chamber

(Rodesch et al., 1997)

germline cell

(Nagoshi et al., 1995)

nuclear chromosome

(Tirronen et al., 1995)

nurse cell

(Rodesch et al., 1997)

nurse cell & nuclear chromosome

(Heino et al., 1995)

ovariole

(Pauli et al., 1993, Storto and King, 1987, King et al., 1986)

ovary

(Bae et al., 1994, Steinhauer and Kalfayan, 1992)

Detailed Description

Statement

Reference

Cytoplasmic dumping is blocked in hemizygous egg chambers. The cytoplasmic actin cytoskeleton of nurse cells shows major disruptions, while the cortical actin appears normal in homozygous females.

(Rodesch et al., 1997)

Chromatin organization in the pseudonurse cells differs slightly from wild type. Oligotene fibrils and occasional polytene chromosomes are interspersed with wild-type chromatin fibers.

(Heino et al., 1995)

When XY flies are transformed into pseudofemales by tra^Hsp83.PS, otu activity is sufficient to allow XY germ cell proliferation. Frequency of tumorous egg cysts is approximately equal to that of otu⁺/Y pseudofemales.

(Nagoshi et al., 1995)

DIF (differentiated) class allele.

(Tirronen et al., 1995)

Late stage arrest of oogenesis.

(Bae et al., 1994)

Spermatogenesis normal but mating behaviour fails because wild type females refuse to react to the courtship attempts of mutant males. Effect is not absolute, and depends on allele (there is a strong correlation between male sterility and severity of impairment in the female phenotype) and varies between affecting 6.7% (otu¹⁴) and 86.6% (otu¹²) of mutant males.

(Tirronen et al., 1993)

Homozygous and hemizygous females have a differentiated ovary phenotype.

(Steinhauer and Kalfayan, 1992)

97% of homozygous ovarioles produce nurse cell/oocyte syncytia. Most otu¹¹/otu¹⁴ or otu¹⁴/otu¹³ female flies are fertile.

(Storto and King, 1987)

Differentiated allele; 97% of homozygous ovarioles contain differentiated cystocytes forming oocytes. Over half of hemizygous ovarioles contain differentiated cystocytes (forming pseudonurse cells, nurse cells or oocytes).

(King et al., 1986)

differentiated; ovarian chambers with pseudonurse cells or nurse cell/oocyte synctia

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

otu¹⁴ is rescued by otu^hs.PN

(Rodesch et al., 1995)

Comments

One or two copies of P{hs-otu.N} completely rescues both fertility and fecundity.

(Rodesch et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6025

otu¹⁴/FM3

Notes on Origin

Discoverer

Vyse.

Comments

Differentiated (DIF) class allele. The ovarian phenotype of flies transheterozygous for otu¹⁴ and a number of other otu mutations has been analysed.

(Storto and King, 1987)

Differentiated class (DIF) of otu alleles.

(Steinhauer and Kalfayan, 1992)

The only allele of otu of 13 tested that failed to show a dominant interaction with ovo^D2 or ovo^D3.

(Pauli et al., 1993)

Weak DIF class of otu allele. P{otu-98} and P{otu-104} completely restore fertility.

(Sass et al., 1995)

Weak otu allele.

(Rodesch et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

fs(1)4077

fs4077

(King et al., 1986)

otu¹⁴

(Popodi et al., 2010-, Christensen et al., 2009.6.15, Christensen et al., 2008.12.28, Cook et al., 2008.9.3, Cook et al., 2008.9.3)

Name Synonyms

Secondary FlyBase IDs

References (19)

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