From: Kevin Cook <kercook@XXXX> Date: 15 June 2009 13:13:53 BST To: FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchrist@XXXX> Subject: Isolation and characterization of Df(1)BSC825 Isolation and characterization of Df(1)BSC825 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(1)BSC825 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG1632d05362 and PBac{WH}CG10962f02373. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118 P{XP}CG1632d05362/ w1118 PBac{WH}CG10962f02373; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC825 from the segment of P{XP}CG1632d05362 to the left of its FRT site and the segment of PBac{WH}CG10962f02373 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(1)BSC825 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are X:8165447 ;8932262 and the cytological breakpoints predicted from these coordinates are 7E1;8C4. It failed to complement Cp36dec2-1 and otu14. -- Kevin Cook, Ph.D Bloomington Drosophila Stock Center Department of Biology Indiana University 1001 E. Third St. Bloomington, IN 47405-7005 kercook@XXXX 812-856-1213 (office), 812-855-2577 (fax) http://flystocks.bio.indiana.edu
