FlyBase Allele Report: Dmel\otu[13]

General Information

Symbol

Dmel\otu¹³

Species

D. melanogaster

Name

FlyBase ID

FBal0013360

Feature type

allele

Associated gene

Dmel\otu

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Rodesch et al., 1997)

Mutagen

ethyl methanesulfonate

(Storto and King, 1987, King et al., 1986)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G2779A. This alters the splice acceptor site of exon 6a.

(Tirronen et al., 1995)

Lesion disrupts the splice acceptor site for the exon specific for the 104kD isoform.

(Bae et al., 1994)

Nucleotide substitution: G2779A. This alters the 3' splice acceptor site at the 5' end of the exon 6a from the invariant AG dinucleotide, which is required for splice acceptor site function, to AA.

(Steinhauer and Kalfayan, 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,487,383..8,487,383 [-]

Nucleotide change:

G8487383A

Amino acid change:

G2779A

Comment:

G to A substitution in the splice acceptor for exon 6a.

(Tirronen et al., 1995, Steinhauer and Kalfayan, 1992)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | female

(Tirronen et al., 1993)

female sterile

(Bae et al., 1994)

female sterile | recessive

(Klusza et al., 2013, King et al., 1986)

Phenotype Manifest In

chromatin

(Klusza et al., 2013)

fusome

(Rodesch et al., 1997)

nurse cell

(Klusza et al., 2013)

nurse cell & nuclear chromosome (with otu¹¹)

(Goodrich et al., 2004)

nurse cell ring canal

(Rodesch et al., 1997)

ovariole

(Pauli et al., 1993, Storto and King, 1987, King et al., 1986)

ovary

(Bae et al., 1994, Steinhauer and Kalfayan, 1992)

Detailed Description

Statement

Reference

otu¹³/otu¹³ females are sterile. otu¹³/+ females are fertile, but nurse cells in egg chambers in these females exhibit the 5-blob phenotype - a failure of nurse cell chromosome dispersal from stage 6 onwards, with the major chromosome arms remaining condensed, in contrast to wild type, but egg chambers do not exhibit any defects in oocyte polarization.

(Klusza et al., 2013)

Unlike in wild-type females, polytene chromosomes in the nurse cells of otu¹¹/otu¹³ females fail to disperse after stage 6.

(Goodrich et al., 2004)

The ring canals are devoid of fusome material and have inner rims that are indistinguishable from wild-type in homozygous females. Ring canals lacking both fusomes and an inner ring are also seen in these females and a number of ring canals that are a fraction of wild-type size are occasionally seen. Fusomes contain actin filaments, and are generally linear or unbranched.

(Rodesch et al., 1997)

Tumorous phenotype.

(Rodesch et al., 1995)

ONC class allele.

(Tirronen et al., 1995)

Tumorous ovaries. Mutant phenotype can be suppressed by Sxl^M1, and some egg cysts show partial fertility and fecundity. otu¹¹/otu¹³ females are completely sterile, producing small ovaries containing mostly tumorous egg cysts. Transheterozygotes with otu² or otu¹⁰ produce ovaries containing predominantly tumorous egg cysts.

(Bae et al., 1994)

Spermatogenesis normal but mating behaviour fails because wild type females refuse to react to the courtship attempts of mutant males. Effect is not absolute, and depends on allele (there is a strong correlation between male sterility and severity of impairment in the female phenotype) and varies between affecting 6.7% (otu¹⁴) and 86.6% (otu¹²) of mutant males.

(Tirronen et al., 1993)

Homozygous and hemizygous females have tumorous ovaries.

(Steinhauer and Kalfayan, 1992)

65.5% of homozygous ovarioles have tumorous oogonia, and 18.5% produce pseudonurse cell chambers with no oocyte. Most otu¹⁴/otu¹³ female flies are fertile. otu¹³/otu⁵ female flies are poorly fertile.

(Storto and King, 1987)

Oncogenic allele; approximately half of homozygous and hemizygous ovarioles contain tumour-generating oogonia. Substantial numbers of differentiated cystocytes are also produced.

(King et al., 1986)

oncogenic; cystocytes continue dividing and produce tumors

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

otu¹³ has female sterile phenotype, non-suppressible by Sxl^M1

(Bae et al., 1994)

Other

Statement

Reference

Sxl^M1, otu¹¹/otu¹³ has female fertile phenotype

(Bae et al., 1994)

Phenotype Manifest In

Enhanced by

Statement

Reference

otu¹³ has nurse cell phenotype, enhanceable by Df(2R)Exel7130/+

(Klusza et al., 2013)

otu¹³ has chromatin phenotype, enhanceable by Df(2R)Exel7130/+

(Klusza et al., 2013)

otu¹³ has nurse cell phenotype, enhanceable by pea[+]/pea¹

(Klusza et al., 2013)

otu¹³ has chromatin phenotype, enhanceable by pea[+]/pea¹

(Klusza et al., 2013)

Suppressed by

Statement

Reference

otu¹³ has ovary phenotype, suppressible by Sxl^M1

(Bae et al., 1994)

Additional Comments

Genetic Interactions

Statement

Reference

Df(2R)Exel7130/+ or pea¹/+ enhances the nurse cell chromatin defects seen in otu¹³/+ females.

(Klusza et al., 2013)

There are no changes in nurse cell chromosomal dispersal in otu¹³/+; poly² germ-line clones compared to wild-type.

(Klusza and Deng, 2010)

The addition of Sxl^M1 to otu¹¹/otu¹³ females allows fertility and high fecundity. Sxl^M1 incompletely suppresses the otu²/otu¹³ or otu¹⁰/otu¹³ mutant phenotype, ovaries are at a more advanced stage of oogenesis but females are still completely sterile.

(Bae et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

otu[+]/otu¹³ is rescued by otu¹⁰⁴

(Klusza et al., 2013)

Partially rescued by

otu¹³ is partially rescued by otu^hs.PN

(Rodesch et al., 1995)

Comments

Expression of otu¹⁰⁴ rescues the nurse cell chromatin dispersal failure seen in otu¹³/+ mutant females.

(Klusza et al., 2013)

One or two copies of P{hs-otu.N} causes substantial morphological suppression but only partial suppression of sterility.

(Rodesch et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6024

y¹ cv¹ otu¹³ v¹ f¹/FM3

Notes on Origin

Discoverer

Mohler.

Comments

Oncogenic (ONC) class allele. The ovarian phenotype of flies transheterozygous for otu¹³ and a number of other otu mutations has been analysed.

(Storto and King, 1987)

Oncogenic class (ONC) of otu allele.

(Steinhauer and Kalfayan, 1992)

Shows a dominant interaction with ovo^D2 and ovo^D3 : more extreme mutant phenotype as assayed by reduction in number of egg chambers at stage 10 or further developed. There is no apparent correlation with strength of otu allele and its interaction with the ovo alleles.

(Pauli et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

13F-3

(Mohler and Carroll, 1984)

fs(1)13F-3

fs13F-3

(King et al., 1986)

otu¹³

(Klusza et al., 2013, Klusza and Deng, 2010, Sun and Cline, 2009)

Name Synonyms

Secondary FlyBase IDs

References (15)

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