FlyBase Allele Report: Dmel\otu[11]

General Information

Symbol

Dmel\otu¹¹

Species

D. melanogaster

Name

FlyBase ID

FBal0013358

Feature type

allele

Associated gene

Dmel\otu

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Storto and King, 1987, King et al., 1986)

Progenitor genotype

Cytology

Description

Single missense mutation in the exon specific to the 104kD isoform.

(Bae et al., 1994)

Nucleotide substitution: G2793A. This causes the amino acid at position 5A of the alternate exon to change from Cys to Tyr.

(Steinhauer and Kalfayan, 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,487,369..8,487,369 [-]

Nucleotide change:

G8487369A

Reported nucleotide change:

G2793A

Amino acid change:

C343Y | otu-PB; C343Y | otu-PC

Reported amino acid change:

C?Y

(Steinhauer and Kalfayan, 1992)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | female

(Tirronen et al., 1993)

female fertile (with otu⁴)

(Storto and King, 1987)

female sterile

(Bae et al., 1994)

female sterile | recessive

(Mal'ceva et al., 1997, King et al., 1986)

Phenotype Manifest In

dorsal appendage

(Van Buskirk and Schupbach, 2002)

egg

(Van Buskirk and Schupbach, 2002)

egg chamber

(Keyes and Spradling, 1997)

fusome

(Lin et al., 1994)

germarium

(Van Buskirk and Schupbach, 2002, Keyes and Spradling, 1997)

nurse cell

(Mal'ceva et al., 1997)

nurse cell & nuclear chromosome (with otu¹³)

(Goodrich et al., 2004)

ovariole

(Pauli et al., 1993, Storto and King, 1987, King et al., 1986)

ovary

(Starr and Cline, 2002, Bae et al., 1994, Steinhauer and Kalfayan, 1992)

polytene chromosome band & nurse cell | ectopic

(Koryakov et al., 2003)

Detailed Description

Statement

Reference

Unlike in wild-type females, polytene chromosomes in the nurse cells of otu¹¹/otu¹³ females fail to disperse after stage 6.

(Goodrich et al., 2004)

In the nurse cell polytene chromosomes of otu¹¹ mutants have some banding which is distinct from wild-type: the proximal 3L euchromatin comprises diffuse bands in section 80, whereas the proximal 3R contains a distinct band in 81F. Novel chromosomal material, visible between sections 80 and 81 contains a number of constrictions which were frequently the site of chromosome breakage.

(Koryakov et al., 2003)

77% of ovaries of homozygous females that are not infected with Wolbachia have no eggs and 23% of ovaries have 1-2 eggs. The ovary phenotypes of homozygous females are not suppressed by Wolbachia infection.

(Starr and Cline, 2002)

otu⁷/otu¹¹ females do produce differentiated egg chambers. Homozygous females produce tumorous germaria, but under certain conditions produce rare eggs which show defects including the expansion of dorsal appendages.

(Van Buskirk and Schupbach, 2002)

ONC class temperature-sensitive allele. Homozygous females have ovarioles with both tumorous germaria and polytenised late egg chambers arrested at about stage 12.

(Keyes and Spradling, 1997)

The nurse cells of homozygous females contain giant polytene chromosomes of various morphologies. otu⁷/otu¹¹ females are fertile and their nurse cells often contain polytene chromosomes, which are comparable to and often larger than normal salivary gland chromosomes.

(Mal'ceva et al., 1997)

Tumorous ovaries. otu¹¹/otu¹³ females are completely sterile, producing small ovaries containing mostly tumorous egg cysts.

(Bae et al., 1994)

Germaria and egg chambers from otu¹¹ females show branched fusome or greatly increased numbers of small cells containing early fusomes. Some of the tumor cells still acquire a follicle cell layer.

(Lin et al., 1994)

Spermatogenesis normal but mating behaviour fails because wild type females refuse to react to the courtship attempts of mutant males. Effect is not absolute, and depends on allele (there is a strong correlation between male sterility and severity of impairment in the female phenotype) and varies between affecting 6.7% (otu¹⁴) and 86.6% (otu¹²) of mutant males.

(Tirronen et al., 1993)

Homozygous and hemizygous females have tumorous ovaries.

(Steinhauer and Kalfayan, 1992)

21% of homozygous ovarioles have quiescent oogonia, 59% have tumorous oogonia, and 15% produce pseudonurse cell chambers with no oocyte. Most otu¹¹/otu¹⁴, otu⁷/otu¹¹, otu¹¹/otu⁵ or otu¹¹/otu⁴ female flies are fertile. otu⁸/otu¹¹, otu¹¹/otu⁷, otu¹¹/otu³ and otu⁹/otu¹¹ female flies are poorly fertile. Oncogenic (ONC) class allele.

(Storto and King, 1987)

Oncogenic allele; over half of homozygous and hemizygous ovarioles contain tumour-generating oogonia. Substantial numbers of differentiated cystocytes are also produced.

(King et al., 1986)

oncogenic; cystocytes continue dividing and produce tumors

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

otu¹¹ has female sterile phenotype, suppressible by Sxl^M1

(Bae et al., 1994)

Suppressor of

Statement

Reference

otu[+]/otu¹¹ is a suppressor of abnormal mitotic cell cycle phenotype of enc^UU3

(Van Buskirk and Schupbach, 2002)

Other

Statement

Reference

cup¹⁵, otu[+]/otu¹¹ has female fertile phenotype

(Keyes and Spradling, 1997)

cup¹⁵, otu¹¹ has female fertile phenotype

(Keyes and Spradling, 1997)

Sxl^M1, otu¹¹/otu¹³ has female fertile phenotype

(Bae et al., 1994)

Sxl^M1, otu¹¹ has female fertile phenotype

(Bae et al., 1994)

Phenotype Manifest In

Enhanced by

Statement

Reference

otu¹¹ has egg chamber phenotype, enhanceable by cup¹⁵/cup¹⁵

(Keyes and Spradling, 1997)

Enhancer of

Statement

Reference

otu¹¹ is an enhancer of phenotype of ct^C145

(Jackson and Berg, 1999)

otu[+]/otu¹¹ is an enhancer of egg chamber phenotype of ct^C145

(Jackson and Berg, 1999)

otu¹¹ is an enhancer of egg chamber phenotype of cup¹⁵

(Keyes and Spradling, 1997)

Suppressor of

Statement

Reference

otu[+]/otu¹¹ is a suppressor of egg chamber phenotype of enc^UU3

(Van Buskirk and Schupbach, 2002)

otu¹¹ is a suppressor of egg chamber phenotype of cup³²

(Keyes and Spradling, 1997)

otu[+]/otu¹¹ is a suppressor of egg chamber phenotype of cup⁴

(Keyes and Spradling, 1997)

Other

Statement

Reference

cup¹⁵, otu¹¹ has nurse cell & chromatin phenotype

(Keyes and Spradling, 1997)

cup¹⁵, otu[+]/otu¹¹ has nurse cell & chromatin phenotype

(Keyes and Spradling, 1997)

cup¹⁵, otu¹¹ has ovariole phenotype

(Keyes and Spradling, 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The extra germline cell phenotype seen in enc^UU3 egg chambers is suppressed by otu¹¹/+.

(Van Buskirk and Schupbach, 2002)

ct^C145/+ ; otu¹¹/+ egg chambers contain binucleate cells. The majority of egg chambers contain a single binucleate cell, which can be positioned anywhere in the egg chamber, although occasionally more than one binucleate cell per egg chamber is seen.

(Jackson and Berg, 1999)

cup¹⁵ otu¹¹ double homozygotes (raised at the restrictive temperature for otu¹¹) have a more severe phenotype than either single mutant homozygote: ovarioles are either extremely tumorous, with no budded chambers, or appear completely agametic. cup¹⁵/cup¹⁵ otu¹¹/+ females (raised at the restrictive temperature for otu¹¹) are fertile, although some eggs laid have flaccid chorions and do not hatch. Nurse cell chromatin morphology is not completely normal in these females and 18% of egg chambers are dicephalic. One copy of otu¹¹ improves the phenotype of cup⁴ and cup³² homozygotes, allowing egg chamber growth to a later developmental stage with more uniform chromatin morphologies. Many egg chambers in these flies are dicephalic.

(Keyes and Spradling, 1997)

The addition of Sxl^M1 to otu¹¹/otu¹³ females allows fertility and high fecundity.

(Bae et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3046

y¹ cv¹ otu¹¹ v¹ f¹/FM3

Kyoto

106803

y¹ cv¹ otu¹¹ v¹ f¹/FM3

Notes on Origin

Discoverer

Mohler.

Comments

Oncogenic class (ONC) of otu allele.

(Steinhauer and Kalfayan, 1992)

The degree of underreplication of intercalary heterochromatin is lower in polytene pseudonurse cell chromosomes than in polytene salivary gland chromosomes. In chromosome 3R a large mass of granular heterochromatin is observed proximal to region 81F, the pattern of additional heterochromatin is highly reproducible. Other heterochromatic blocks are present on the X and second chromosome and unattached, all blocks are well banded.

(Malceva and Zhimulev, 1993)

Shows a dominant interaction with ovo^D2 and ovo^D3 : more extreme mutant phenotype as assayed by reduction in number of egg chambers at stage 10 or further developed. There is no apparent correlation with strength of otu allele and its interaction with the ovo alleles.

(Pauli et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

14-334

(Mohler and Carroll, 1984)

fs(1)14-334

fs14-334

(King et al., 1986)

otu11

(Le Thomas et al., 2013)

otu¹¹

(Casier et al., 2023, Koryakov et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (26)

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