A Tag:HA epitope is fused to the N-terminal end of the open reading frame, and the GxxxxGKT box within the helicase domain is mutated to GxxxxGNT.
partially lethal - majority live | male
wild-type
P{HA-GNT} males have a 50% lower viability compared to sisters. P{HA-GNT} is able to rescue mle1 males.
mleGNT.Tag:HA rescues mle1
Rescues the lethality of mle1 males.