A Tag:HA epitope is fused to the N-terminal end of the open reading frame, and the SAT box within the helicase domain is mutated to AAA.
wild-type
P{HA-AAA} males survived in equal numbers to their sisters. P{HA-AAA} is able to rescue mle1 males.
mleAAA.Tag:HA rescues mle1
Rescues the lethality of mle1 males.