FlyBase Allele Report: Dmel\mod(mdg4)[neo129]

General Information

Symbol

Dmel\mod(mdg4)^neo129

Species

D. melanogaster

Name

FlyBase ID

FBal0003361

Feature type

allele

Associated gene

Dmel\mod(mdg4)

Associated Insertion(s)

P{hsneo.ry⁺}mod(mdg4)^neo129

Carried in Construct

Also Known As

mod(mdg4)^E(var)3-93D, E(var)3-93D

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Buchner et al., 2000)

Mutagen

P-element activity

(Buchner et al., 2000)

Delta2-3 transposase

(Dorn et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{hsneo.ry⁺}mod(mdg4)^neo129

Cytology

Description

Insertion of P{lacW} in the third intron at nucleotide 153,036 of AE003734.

(Mongelard et al., 2002)

Insertion into the third intron, which separates coding exon 2 from coding exon 3 of the mod(mdg4) transcription unit. The insertion lies 121 bp downstream from the 3' junction of coding exon 2.

(Buchner et al., 2000)

Insertion of a P-element into the third intron, 121bp downstream to the 5' splice junction.

(Dorn et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | male (with mod(mdg4)^Z3-3401)

(Soltani-Bejnood et al., 2007)

decreased fecundity | female

(Buchner et al., 2000)

enhancer of variegation | dominant, with In(1)w^m4

(Dorn et al., 1993, Dorn et al., 1993)

enhancer of variegation | dominant, with P{hsp26-pt-T}39C-42

(Sun et al., 2000)

enhancer of variegation | dominant, with P{hsp26-pt-T}118E-25-5

(Sun et al., 2000)

female sterile

(Dorn et al., 1993)

female sterile (with mod(mdg4)⁰³)

(Buchner et al., 2000)

homeotic | pharate adult stage (with mod(mdg4)^m9)

(Savitsky et al., 2016)

lethal (with mod(mdg4)⁰²)

(Gabler et al., 2005)

lethal (with mod(mdg4)⁰⁷)

(Gabler et al., 2005)

lethal | cold sensitive

(Dorn et al., 1993)

lethal | recessive

(Gabler et al., 2005)

lethal - all die before end of larval stage | recessive

(Mongelard et al., 2002)

lethal - all die before end of P-stage (with mod(mdg4)^m9)

(Savitsky et al., 2016)

lethal - all die before end of pupal stage (with mod(mdg4)^T16)

(Chen and Corces, 2001)

lethal - all die before end of pupal stage | recessive

(Gerasimova and Corces, 1998)

lethal - all die before end of pupal stage | recessive | heat sensitive

(Buchner et al., 2000)

lethal - all die during prepupal stage

(Savitsky et al., 2016)

majority die during prepupal stage (with mod(mdg4)^m9)

(Savitsky et al., 2016)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}39C-12

(Sun et al., 2000)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}118E-3

(Sun et al., 2000)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}118E-10

(Sun et al., 2000)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}118E-19

(Sun et al., 2000)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}Abcd1^39C-34

(Sun et al., 2000)

non-suppressor of variegation | dominant, with T(2;3)lt^X13

(Weiler and Wakimoto, 2002)

partially lethal - majority die

(Soltani-Bejnood et al., 2007)

partially lethal - majority die (with mod(mdg4)⁰³)

(Buchner et al., 2000)

some die during larval stage | recessive

(Mongelard et al., 2002)

some die during pharate adult stage (with mod(mdg4)^m9)

(Savitsky et al., 2016)

some die during pupal stage (with mod(mdg4)^T16)

(Chen and Corces, 2001)

some die during pupal stage | recessive

(Gerasimova and Corces, 1998)

some die during pupal stage | recessive | heat sensitive

(Buchner et al., 2000)

suppressor of variegation | dominant, with In(2L)lt^X2

(Weiler and Wakimoto, 2002)

suppressor of variegation | dominant, with In(2LR)lt^G10

(Weiler and Wakimoto, 2002)

Phenotype Manifest In

abdominal segment 6 | pharate adult stage (with mod(mdg4)^m9)

(Savitsky et al., 2016)

abdominal segment 7 | pharate adult stage (with mod(mdg4)^m9)

(Savitsky et al., 2016)

abdominal segment 8 | pharate adult stage (with mod(mdg4)^m9)

(Savitsky et al., 2016)

abdominal sternite bristle | pharate adult stage (with mod(mdg4)^m9)

(Savitsky et al., 2016)

ovariole

(Buchner et al., 2000)

ovary

(Buchner et al., 2000)

sternite | pharate adult stage (with mod(mdg4)^m9)

(Savitsky et al., 2016)

tergite | pharate adult stage (with mod(mdg4)^m9)

(Savitsky et al., 2016)

Detailed Description

Statement

Reference

mod(mdg4)^m9/mod(mdg4)^neo129 have 75% early pupal (P1-4) lethality, and 25% late pupa to pharate adult lethality (stage 12-15) and pharate adults show homeotic transformations: in males s6 (sternite of the sixth segment) has 3-7 (average 5) bristles, t7 (tergite of the seventh segment) is enlarged and bears several bristles; in females s7 bears 9-11 bristles and occasionally 3 large bristles instead of 2, bristles on s7 occasionally lose orientation, and t8 occasionally bears 1-2 bristles.

(Savitsky et al., 2016)

mod(mdg4)^neo129/+ suppresses the variegation of lt due to In(2LR)lt^G10 (seen in In(2LR)lt^G10/Df(2L)lt10 animals derived from lt¹⁶/Df(2L)lt10 females), so that the percentage of pigmented Malpighian tubule cells is increased in the double mutant larvae. mod(mdg4)^neo129/+ suppresses the variegation of lt due to In(2L)lt^X2 (seen in In(2L)lt^X2/Df(2L)lt10 animals derived from lt¹⁶/Df(2L)lt10 females), so that the percentage of pigmented Malpighian tubule cells is increased in the double mutant larvae. mod(mdg4)^neo129/+ does not suppress the variegation of lt due to T(2;3)lt^X13 (seen in T(2;3)lt^X13/Df(2L)lt10 animals derived from lt¹⁶/Df(2L)lt10 females); there is no significant change in the percentage of pigmented Malpighian tubule.

(Weiler and Wakimoto, 2002)

Enhances the variegation in, i.e. reduces the eye pigmentation of, In(1)w^m4h, Su(var)2-1¹ males. Homozygous females obtained at 29^oC produce a small number of eggs that do not show signs of further development. Ovaries are of various size and ovarioles are fewer than normal and contain fewer egg chambers.

(Buchner et al., 2000)

Does not enhance transinactivation of bw caused by Tp(2;2)bw-DX7.

(Sass and Henikoff, 1999)

Some homozygous escapers develop to the adult stage.

(Gerasimova and Corces, 1998)

Enhances variegation of In(1)w^m4h.

(Dorn et al., 1993)

Strong dominant enhancer effect on w variegation in In(1)w^m4h. Homozygous males display a strong transformation of the fifth into the fourth abdominal segment, weak transformation is displayed by heterozygotes.

(Dorn et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

In(1)w^m4, mod(mdg4)^neo129 has enhancer of variegation phenotype, enhanceable by trx^red-P6/trx[+]

(Buchner et al., 2000)

Suppressed by

Statement

Reference

mod(mdg4)^neo129 has lethal | recessive phenotype, suppressible by Dvir\mod(mdg4)^t11.5

(Gabler et al., 2005)

mod(mdg4)^neo129/mod(mdg4)⁰² has lethal phenotype, suppressible | partially by Dvir\mod(mdg4)^t11.5

(Gabler et al., 2005)

mod(mdg4)^neo129/mod(mdg4)⁰² has lethal phenotype, suppressible | partially by Dvir\mod(mdg4)^t6.8

(Gabler et al., 2005)

mod(mdg4)^neo129/mod(mdg4)^R32 has lethal phenotype, suppressible | partially by Dvir\mod(mdg4)^t11.5

(Gabler et al., 2005)

NOT suppressed by

Statement

Reference

mod(mdg4)⁰⁷/mod(mdg4)^neo129 has lethal phenotype, non-suppressible by Dvir\mod(mdg4)^t11.5

(Gabler et al., 2005)

Enhancer of

Statement

Reference

mod(mdg4)^neo129/mod(mdg4)[+] is an enhancer of visible | homeotic phenotype of E(var)3-4¹

(Buchner et al., 2000)

mod(mdg4)^neo129/mod(mdg4)[+] is an enhancer of enhancer of variegation phenotype of In(1)w^m4, vtd²

(Buchner et al., 2000)

mod(mdg4)^neo129/mod(mdg4)[+] is an enhancer of visible | homeotic phenotype of trx^red-P6

(Buchner et al., 2000)

Suppressor of

Statement

Reference

mod(mdg4)^neo129/mod(mdg4)[+] is a suppressor of suppressor of variegation phenotype of In(1)w^m4h, Su(var)2-1¹

(Buchner et al., 2000)

NOT Suppressor of

Statement

Reference

mod(mdg4)^neo129/mod(mdg4)^ul is a non-suppressor of visible | recessive phenotype of ct⁶

(Mongelard et al., 2002)

mod(mdg4)^neo129/mod(mdg4)^ul is a non-suppressor of abnormal body color | recessive phenotype of y²

(Mongelard et al., 2002)

mod(mdg4)^neo129/mod(mdg4)^ul is a non-suppressor of visible | recessive phenotype of y²

(Mongelard et al., 2002)

Other

Statement

Reference

CTCF^EY15833/CTCF^y+1, mod(mdg4)^neo129/mod(mdg4)^m9 has lethal - all die during prepupal stage phenotype

(Savitsky et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

In(1)w^m4, mod(mdg4)^neo129 has pigment cell phenotype, enhanceable by trx^red-P6/trx[+]

(Buchner et al., 2000)

Enhancer of

Statement

Reference

mod(mdg4)^neo129/mod(mdg4)[+] is an enhancer of adult abdominal segment 5 phenotype of E(var)3-4¹

(Buchner et al., 2000)

mod(mdg4)^neo129/mod(mdg4)[+] is an enhancer of pigment cell phenotype of In(1)w^m4, vtd²

(Buchner et al., 2000)

mod(mdg4)^neo129/mod(mdg4)[+] is an enhancer of adult abdominal segment 5 phenotype of trx^red-P6

(Buchner et al., 2000)

NOT Suppressor of

Statement

Reference

mod(mdg4)^neo129/mod(mdg4)^ul is a non-suppressor of wing phenotype of ct⁶

(Mongelard et al., 2002)

mod(mdg4)^neo129/mod(mdg4)^ul is a non-suppressor of adult epidermis phenotype of y²

(Mongelard et al., 2002)

Other

Statement

Reference

In(1)w^m4h, Su(var)2-1¹, mod(mdg4)^neo129/mod(mdg4)[+] has pigment cell phenotype

(Buchner et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

CTCF^y+1 mod(mdg4)^neo129 / CTCF^EY15833 mod(mdg4)^m9 show early pupal lethality (stage P1-4).

(Savitsky et al., 2016)

Enhances the homeotic transformation of A5 to A4 of trx^red-P6 and E(var)3-4¹.

(Buchner et al., 2000)

Xenogenetic Interactions

Statement

Reference

One copy of Dvir\mod(mdg4)^t11.5 can rescue the lethality of mod(mdg4)^neo129 homozygotes (92% of the expected progeny survive). One copy of Dvir\mod(mdg4)^t11.5 can rescue the lethality of mod(mdg4)⁰²/mod(mdg4)^neo129 transheterozygotes, with the degree of rescue depending on the P{Dvir\mod(mdg4)^t11.5} line used (95.1% and full viability has been seen). One copy of Dvir\mod(mdg4)^t11.5 can rescue the lethality of mod(mdg4)^R32/mod(mdg4)^neo129 transheterozygotes. Rescued males show greater viability (63.2%) compared to rescued females (40.5%). One copy of Dvir\mod(mdg4)^t11.5 does not suppresses the lethality of mod(mdg4)⁰⁷/mod(mdg4)^neo129 transheterozygotes. One copy of Dvir\mod(mdg4)^t6.8 partially rescues the lethality of mod(mdg4)⁰²/mod(mdg4)^neo129 transheterozygotes (34% viability is seen).

(Gabler et al., 2005)

Complementation and Rescue Data

Complements

mod(mdg4)^ul

(Mongelard et al., 2002)

mod(mdg4)^T6

(Mongelard et al., 2002)

Partially complements

mod(mdg4)^Z3-3401

(Soltani-Bejnood et al., 2007)

mod(mdg4)⁰³

(Buchner et al., 2000)

Fails to complement

mod(mdg4)^L3101

(Mongelard et al., 2002)

mod(mdg4)⁰³⁸⁵²

(Mongelard et al., 2002)

mod(mdg4)⁰²

(Buchner et al., 2000)

mod(mdg4)⁰⁴

(Buchner et al., 2000)

mod(mdg4)⁰⁶

(Buchner et al., 2000)

mod(mdg4)⁰⁷

(Buchner et al., 2000)

mod(mdg4)^R32

(Buchner et al., 2000)

mod(mdg4)^bpd1

(Gorczyca et al., 1999)

mod(mdg4)^bpdEX1

(Gorczyca et al., 1999)

Partially rescued by

mod(mdg4)^neo129 is partially rescued by mod(mdg4)^+t7.5

(Soltani-Bejnood et al., 2007)

mod(mdg4)^neo129 is partially rescued by mod(mdg4)^t7.5

(Dorn et al., 2001)

mod(mdg4)^neo129 is partially rescued by mod(mdg4)^+t7.5

(Buchner et al., 2000)

Comments

The semi-lethal phenotype of mod(mdg4)^neo129 is fully complemented by mod(mdg4)^Z3-3401, but mod(mdg4)^neo129 fails to complement mod(mdg4)^Z3-3401 with respect to X-Y chromosome nondisjunction in male meiosis - transheterozygotes show a high level of X-Y nondisjunction (41.2%).

The lethal phenotype of mod(mdg4)^neo129 is fully complemented by mod(mdg4)^Z3-5578 and mod(mdg4)^Z3-3298, and mod(mdg4)^neo129 partially or fully complements these alleles with respect to X-Y chromosome nondisjunction in male meiosis - the transheterozygotes show only a low level of X-Y nondisjunction (frequency is indicated in parentheses after each allele); mod(mdg4)^Z3-5578 (4.46%) and mod(mdg4)^Z3-3298 (7.64%).

(Soltani-Bejnood et al., 2007)

Member of Group I complementation group. Complementation statement based on transheterozygous phenotype in terms of suppression or non-suppression of y² and ct⁶ visible phenotypes.

(Mongelard et al., 2002)

Complementation statements based on viability.

(Buchner et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Alleles of mod(mdg4) show an allelic series for their effect on In(1)w^m4h variegation. From strongest to weakest: mod(mdg4)^R32 = mod(mdg4)⁰⁴ = mod(mdg4)⁰⁶ = mod(mdg4)⁰⁷ = mod(mdg4)^142Δ32 > mod(mdg4)^neo129 = mod(mdg4)⁰² = mod(mdg4)⁰³ > mod(mdg4)⁰⁵.

(Buchner et al., 2000)

Strong enhancer. High spontaneous background rate of E(var) mutations in these experiments allows for the possibility that the P insertion is not the cause of the E(var) phenotype.

(Dorn et al., 1993)

P-element has been precisely excised generating wild type revertants.

(Dorn et al., 1993)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

X75498

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

E(var)3-93D

(Sun et al., 2000, Sass and Henikoff, 1999)

E(var)3-93D-neo129

(Gorczyca et al., 1999)

E(var)3-93D^neo

(Paredes and Maggert, 2009)

E(var)93D^neo129

E(var)93D^neo

(Buchner et al., 1995)

E-var(3)3⁰¹

(Dorn et al., 1993)

mod(mdg4)^E(var)3-93D

(Mongelard et al., 2002, Chen and Corces, 2001, Gerasimova and Corces, 1998)

mod(mdg4)^neo129

(Savitsky et al., 2016)

neo129

(Soltani-Bejnood et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (17)

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