FlyBase Allele Report: Dmel\mod(mdg4)[Z3-3401]

General Information

Symbol

Dmel\mod(mdg4)^Z3-3401

Species

D. melanogaster

Name

FlyBase ID

FBal0212797

Feature type

allele

Associated gene

Dmel\mod(mdg4)

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Soltani-Bejnood et al., 2007)

Mutagen

ethyl methanesulfonate

(Soltani-Bejnood et al., 2007)

Progenitor genotype

Cytology

Description

Amino acid replacement: R224C.

(Soltani-Bejnood et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | male (with mod(mdg4)⁰²)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)⁰³)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)²⁰)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)¹¹⁷)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^142Δ10)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^142Δ15)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^142Δ29)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^142Δ32)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^142Δ33)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^142Δ49)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)²⁶⁹)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)³²⁴)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)³⁴⁰)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^neo129)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^T16)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male | recessive

(Soltani-Bejnood et al., 2007)

female sterile (with Df(3R)GC14)

(Soltani-Bejnood et al., 2007)

viable

(Soltani-Bejnood et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous males show a high frequency of X-Y chromosome nondisjunction in meiosis (31.2%).

Homozygous, mod(mdg4)^Z3-3401/Df(3R)GC14 and mod(mdg4)^Z3-3401/mod(mdg4)^T16 males show a low frequency of 4th chromosome loss in meiosis (3.3%, 5.2% and 7.9% respectively).

mod(mdg4)^Z3-3401/Df(3R)GC14 males show a moderate frequency of chromosome 2 nondisjunction in meiosis (16.9%).

Defects are seen in meiosis I in homozygous spermatocytes; although the chromosomes condense normally, the homologs are frequently unpaired at prometaphase I and metaphase I. Anaphase I is disorganised, with chromosomes migrating to the poles asynchronously. 54% of meiosis I poles have nuclei of unequal sizes, indicative of meiosis I nondisjunction. Meiosis II chromosome morphology and segregation appear normal. Spindle structure and kinetochore function appear normal at both divisions.

Homologous pairing in pre-meiotic spermatogonia and in early prophase I spermatocytes appears normal in mod(mdg4)^Z3-3401/mod(mdg4)^T16 males.

mod(mdg4)^Z3-3401/Df(3R)GC14 females do not show increased X chromosome nondisjunction compared to controls and the frequency of recombination in large intervals on the X, second and third chromosomes in the mutant females is normal.

(Soltani-Bejnood et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

mod(mdg4)^Z3-3401/Df(3R)GC14 is an enhancer of abnormal body color phenotype of y²

(Soltani-Bejnood et al., 2007)

Phenotype Manifest In

Other

Statement

Reference

mod(mdg4)^Z3-3401/mod(mdg4)^T16, y² has chaeta phenotype

(Soltani-Bejnood et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Body colour: y² ; mod(mdg4)^Z3-3401/mod(mdg4)^T16 flies have a y^- phenotype (yellow bristles, wings and bodies).

(Soltani-Bejnood et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

mod(mdg4)^T6

(Soltani-Bejnood et al., 2007)

mod(mdg4)^B2

(Soltani-Bejnood et al., 2007)

Rescued by

mod(mdg4)^Z3-3401 is rescued by mod(mdg4)^MNM.hs.EGFP

(Soltani-Bejnood et al., 2007)

mod(mdg4)^Z3-3401/mod(mdg4)^T16 is rescued by mod(mdg4)^MNM.hs.EGFP

(Soltani-Bejnood et al., 2007)

Partially rescued by

mod(mdg4)^Z3-3401 is partially rescued by mod(mdg4)^+t7.5

(Soltani-Bejnood et al., 2007)

mod(mdg4)^Z3-3401/mod(mdg4)^T16 is partially rescued by mod(mdg4)^+t7.5

(Soltani-Bejnood et al., 2007)

Comments

mod(mdg4)^Z3-3401 fully complements the lethal/semi-lethal phenotypes of the following mod(mdg4) alleles, but they fail to complement mod(mdg4)^Z3-3401 with respect to X-Y chromosome nondisjunction in male meiosis, with the transheterozygotes showing a high level of X-Y nondisjunction (frequency is indicated in parentheses after each allele); mod(mdg4)^T16 (39.6%), mod(mdg4)^142Δ10 (49.3%), mod(mdg4)^142Δ33 (49.1%), mod(mdg4)^142Δ15 (42.1%), mod(mdg4)^142Δ32 (28.3%), mod(mdg4)^142Δ29 (46%), mod(mdg4)^142Δ49 (54.9%), mod(mdg4)^neo129 (41.2%), mod(mdg4)⁰² (32.1%), mod(mdg4)⁰³ (34.9%), mod(mdg4)²⁰ (45.8%), mod(mdg4)¹¹⁷ (47.2%), mod(mdg4)²⁶⁹ (49.5%), mod(mdg4)³²⁴ (44.4%) and mod(mdg4)³⁴⁰ (54.1%).

mod(mdg4)^Z3-3401 fully complements the lethal/semi-lethal phenotypes of the following mod(mdg4) alleles, and they partially or fully complement mod(mdg4)^Z3-3401 with respect to X-Y chromosome nondisjunction in male meiosis, with the transheterozygotes showing only a low level of X-Y nondisjunction (frequency is indicated in parentheses after each allele); mod(mdg4)^eGp4 (2.94%) and mod(mdg4)^B2 (0%).

mod(mdg4)^T6 fully complements mod(mdg4)^Z3-3401 with respect to X-Y chromosome nondisjunction in male meiosis.

mod(mdg4)^Z3-3401 strongly (but not completely) complements mod(mdg4)^Z3-5578 and mod(mdg4)^Z3-3298; transheterozygotes show only a low level of X-Y nondisjunction in male meiosis (frequency is indicated in parentheses after each allele); frequency is mod(mdg4)^Z3-5578 (1.42%) and mod(mdg4)^Z3-3298 (1.09%).

(Soltani-Bejnood et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Z3-3401

(Soltani-Bejnood et al., 2007)

mod(mdg4)^Z3-3401

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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