FlyBase Allele Report: Dmel\mod(mdg4)[02]

General Information

Symbol

Dmel\mod(mdg4)⁰²

Species

D. melanogaster

Name

FlyBase ID

FBal0117437

Feature type

allele

Associated gene

Dmel\mod(mdg4)

Associated Insertion(s)

gypsy{}mod(mdg4)⁰²

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

spontaneous

Nature of the Allele

Allele class

loss of function allele

(Buchner et al., 2000)

Mutagen

spontaneous

(Buchner et al., 2000)

Progenitor genotype

Associated Insertion(s)

gypsy{}mod(mdg4)⁰²

Cytology

Description

Insertion into the third intron, which separates coding exon 2 from coding exon 3 of the mod(mdg4) transcription unit, 84bp downstream from the exon/intron junction.

(Buchner et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | male (with mod(mdg4)^Z3-3401)

(Soltani-Bejnood et al., 2007)

female sterile (with mod(mdg4)⁰³)

(Buchner et al., 2000)

lethal

(Soltani-Bejnood et al., 2007)

lethal (with mod(mdg4)⁰⁷)

(Gabler et al., 2005)

lethal (with mod(mdg4)^neo129)

(Gabler et al., 2005)

lethal (with mod(mdg4)^R32)

(Gabler et al., 2005)

lethal | recessive

(Gabler et al., 2005)

lethal - all die before end of larval stage | recessive

(Buchner et al., 2000)

non-suppressor of variegation | dominant, with T(2;3)lt^X13

(Weiler and Wakimoto, 2002)

partially lethal - majority die (with mod(mdg4)⁰³)

(Buchner et al., 2000)

some die during larval stage | recessive

(Buchner et al., 2000)

suppressor of variegation | dominant, with In(2L)lt^X2

(Weiler and Wakimoto, 2002)

suppressor of variegation | dominant, with In(2LR)lt^G10

(Weiler and Wakimoto, 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

mod(mdg4)⁰²/+ suppresses the variegation of lt due to In(2LR)lt^G10 (seen in In(2LR)lt^G10/Df(2L)lt10 animals derived from lt¹⁶/Df(2L)lt10 females), so that the percentage of pigmented Malpighian tubule cells is increased in the double mutant larvae. mod(mdg4)⁰²/+ suppresses the variegation of lt due to In(2L)lt^X2 (seen in In(2L)lt^X2/Df(2L)lt10 animals derived from lt¹⁶/Df(2L)lt10 females), so that the percentage of pigmented Malpighian tubule cells is increased in the double mutant larvae. mod(mdg4)⁰²/+ does not suppress the variegation of lt due to T(2;3)lt^X13 (seen in T(2;3)lt^X13/Df(2L)lt10 animals derived from lt¹⁶/Df(2L)lt10 females); there is no significant change in the percentage of pigmented Malpighian tubule. mod(mdg4)⁰²/+ suppresses the variegation of lt due to In(2LR)lt^G10 (seen in In(2LR)lt^G10/lt¹ animals), so that the amount of eye pigment is increased in the double mutant adults.

(Weiler and Wakimoto, 2002)

Enhances the variegation in, i.e. reduces the eye pigmentation of, In(1)w^m4h, Su(var)2-1¹ males.

(Buchner et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mod(mdg4)^neo129/mod(mdg4)⁰² has lethal phenotype, suppressible | partially by Dvir\mod(mdg4)^t11.5

(Gabler et al., 2005)

mod(mdg4)^neo129/mod(mdg4)⁰² has lethal phenotype, suppressible | partially by Dvir\mod(mdg4)^t6.8

(Gabler et al., 2005)

mod(mdg4)⁰⁷/mod(mdg4)⁰² has lethal phenotype, suppressible | partially by Dvir\mod(mdg4)^t11.5

(Gabler et al., 2005)

mod(mdg4)⁰²/mod(mdg4)^R32 has lethal | male phenotype, suppressible | partially by Dvir\mod(mdg4)^t6.8

(Gabler et al., 2005)

mod(mdg4)⁰²/mod(mdg4)^R32 has lethal phenotype, suppressible | partially by Dvir\mod(mdg4)^t11.5

(Gabler et al., 2005)

mod(mdg4)⁰² has lethal | recessive phenotype, suppressible by Dvir\mod(mdg4)^t6.8

(Gabler et al., 2005)

mod(mdg4)⁰² has lethal | recessive phenotype, suppressible by Dvir\mod(mdg4)^t11.5

(Gabler et al., 2005)

NOT suppressed by

Statement

Reference

mod(mdg4)⁰⁷/mod(mdg4)⁰² has lethal phenotype, non-suppressible by Dvir\mod(mdg4)^t6.8

(Gabler et al., 2005)

mod(mdg4)⁰²/mod(mdg4)^R32 has lethal | female phenotype, non-suppressible by Dvir\mod(mdg4)^t6.8

(Gabler et al., 2005)

Enhancer of

Statement

Reference

mod(mdg4)[+]/mod(mdg4)⁰² is an enhancer of visible | homeotic phenotype of trx^red-P6

(Buchner et al., 2000)

Phenotype Manifest In

Enhancer of

Statement

Reference

mod(mdg4)[+]/mod(mdg4)⁰² is an enhancer of adult abdominal segment 5 phenotype of trx^red-P6

(Buchner et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Enhances the homeotic transformation of A5 to A4 of trx^red-P6.

(Buchner et al., 2000)

Xenogenetic Interactions

Statement

Reference

One copy of Dvir\mod(mdg4)^t11.5 can rescue the lethality of mod(mdg4)⁰² homozygotes, with the degree of rescue depending on the P{Dvir\mod(mdg4)^t11.5} line used (26.6% and 79.0% viability has been seen). Two copies of Dvir\mod(mdg4)^t11.5 completely suppress the lethality of mod(mdg4)⁰² homozygotes. One copy of Dvir\mod(mdg4)^t11.5 can rescue the lethality of mod(mdg4)⁰²/mod(mdg4)^neo129 transheterozygotes, with the degree of rescue depending on the P{Dvir\mod(mdg4)^t11.5} line used (95.1% and full viability has been seen). One copy of Dvir\mod(mdg4)^t11.5 can rescue the lethality of mod(mdg4)^R32/mod(mdg4)⁰² transheterozygotes. Rescued males show greater viability (91.8% or 95.9% depending on the P{Dvir\mod(mdg4)^t11.5} line used) compared to rescued females (26.2% or 37.8% depending on the P{Dvir\mod(mdg4)^t11.5} line used). The lethality of mod(mdg4)⁰⁷/mod(mdg4)⁰² transheterozygotes is only slightly suppressed by one copy of Dvir\mod(mdg4)^t11.5 (12.5% or 5.4% viability has been seen). One copy of Dvir\mod(mdg4)^t6.8 partially rescues the lethality of mod(mdg4)⁰² homozygotes (10.7% viability is seen). Salivary glands and nuclei are reduced in size and show changes in chromosome morphology in these animals at the larval stage. 74.0% viability is seen in mod(mdg4)⁰² homozygotes carrying two copies of Dvir\mod(mdg4)^t6.8. One copy of Dvir\mod(mdg4)^t6.8 partially rescues the lethality of mod(mdg4)⁰²/mod(mdg4)^neo129 transheterozygotes (34% viability is seen). One copy of Dvir\mod(mdg4)^t6.8 partially rescues the lethality of mod(mdg4)^R32/mod(mdg4)⁰² transheterozygous males (52.3% viability is seen), but the lethality of mod(mdg4)^R32/mod(mdg4)⁰² females is not rescued. One copy of Dvir\mod(mdg4)^t6.8 does not suppresses the lethality of mod(mdg4)⁰⁷/mod(mdg4)⁰² transheterozygotes.

(Gabler et al., 2005)

Complementation and Rescue Data

Partially complements

mod(mdg4)^Z3-3401

(Soltani-Bejnood et al., 2007)

mod(mdg4)⁰³

(Buchner et al., 2000)

Fails to complement

mod(mdg4)⁰⁴

(Buchner et al., 2000)

mod(mdg4)⁰⁶

(Buchner et al., 2000)

mod(mdg4)⁰⁷

(Buchner et al., 2000)

mod(mdg4)^R32

(Buchner et al., 2000)

mod(mdg4)^neo129

(Buchner et al., 2000)

Partially rescued by

mod(mdg4)⁰² is partially rescued by mod(mdg4)^+t7.5

(Buchner et al., 2000)

Comments

The lethal phenotype of mod(mdg4)⁰² is fully complemented by mod(mdg4)^Z3-3401, but mod(mdg4)⁰² fails to complement mod(mdg4)^Z3-3401 with respect to X-Y chromosome nondisjunction in male meiosis - transheterozygotes show a high level of X-Y nondisjunction (32.1%).

The lethal phenotype of mod(mdg4)⁰² is fully complemented by mod(mdg4)^Z3-5578 and mod(mdg4)^Z3-3298, and mod(mdg4)⁰² partially or fully complements these alleles with respect to X-Y chromosome nondisjunction in male meiosis - the transheterozygotes show only a low level of X-Y nondisjunction (frequency is indicated in parentheses after each allele); mod(mdg4)^Z3-5578 (1.72%) and mod(mdg4)^Z3-3298 (2.56%).

(Soltani-Bejnood et al., 2007)

Complementation statements based on viability.

(Buchner et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Alleles of mod(mdg4) show an allelic series for their effect on In(1)w^m4h variegation. From strongest to weakest: mod(mdg4)^R32 = mod(mdg4)⁰⁴ = mod(mdg4)⁰⁶ = mod(mdg4)⁰⁷ = mod(mdg4)^142Δ32 > mod(mdg4)^neo129 = mod(mdg4)⁰² = mod(mdg4)⁰³ > mod(mdg4)⁰⁵.

(Buchner et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

(Soltani-Bejnood et al., 2007)

mod(mdg4)⁰²

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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