FlyBase Allele Report: Scer\GAL4[cry.PZ]

General Information

Symbol

Scer\GAL4^cry.PZ

Species

S. cerevisiae

Name

cryptochrome promoter construct of Zhao

FlyBase ID

FBal0147468

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{cry-GAL4.Z}

Also Known As

Cry-GAL4, crypGAL4, cry16-GAL4, Cry24-gal4, crypGAL4-24

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Zhao et al., 2003)

Progenitor genotype

Carried in construct

P{cry-GAL4.Z}

Cytology

Description

A NotI/NcoI fragment containing the promoter and a fraction of the first exon of cry are fused upstream of sequence encoding a GAL4 driver.

(Zhao et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

cry

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval s-LNv neuron

(Shafer et al., 2006)

larval DN1 neuron

(Shafer et al., 2006)

adult stage

Pdf negative s-LNv neuron

(Helfrich-Forster et al., 2007)

LN Pdf neuron

(Helfrich-Forster et al., 2007)

DN1 neuron | subset

(Helfrich-Forster et al., 2007)

DN3 neuron | subset

(Helfrich-Forster et al., 2007)

Additional Information

Statement

Reference

Drives expression in a subset of pacemaker neurons.

(FlyBase Curators, 2017)

Expression is within all LN neurons, a subset of the DN1 and DN3 neurons, and not at all in the DN2 neurons. Expression was not seen in the third instar larval Pdf negative s-LNv neuron. Fibres of the Pdf negative s-LNV neuron were observed to project towards the accessory medulla and arborize in its central area.

(Helfrich-Forster et al., 2007)

Marker for

Reflects expression of

cry

(Helfrich-Forster et al., 2007)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian behavior, with IscU^GD14694

(Marelja et al., 2018)

abnormal circadian behavior, with Mocs2B^KK101263

(Marelja et al., 2018)

abnormal circadian rhythm, with Clk^UAS.cZa

(Zhao et al., 2003)

abnormal circadian rhythm, with Mef2^EnR.UAS

(Vrailas-Mortimer et al., 2014)

abnormal circadian rhythm, with p38b^ala.UAS

(Vrailas-Mortimer et al., 2014)

abnormal circadian rhythm, with p38b^DD.UAS

(Vrailas-Mortimer et al., 2014)

abnormal circadian rhythm, with p38b^{kd.UAS.Tag:FLAG}

(Vrailas-Mortimer et al., 2014)

abnormal circadian rhythm, with p38b^UAS.cAa

(Vrailas-Mortimer et al., 2014)

abnormal locomotor rhythm, with CG8043^KK107835

(Mandilaras and Missirlis, 2012)

abnormal locomotor rhythm, with Clk^UAS.cZa

(Zhao et al., 2003)

abnormal locomotor rhythm, with Coprox^KK101688

(Mandilaras and Missirlis, 2012)

abnormal locomotor rhythm, with Fer2LCH^KK103577

(Mandilaras and Missirlis, 2012)

abnormal locomotor rhythm, with HisT^KK109206

(Mandilaras and Missirlis, 2012)

abnormal locomotor rhythm, with IscU^GD14694

(Mandilaras and Missirlis, 2012)

abnormal locomotor rhythm, with na^UAS.cLa

(Lear et al., 2005)

abnormal locomotor rhythm, with Nfs1^KK101134

(Mandilaras and Missirlis, 2012)

abnormal locomotor rhythm, with Nubp2^KK100349

(Mandilaras and Missirlis, 2012)

abnormal locomotor rhythm, with Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}

(Choi et al., 2009)

abnormal locomotor rhythm, with per^UAS.cYa

(Zhao et al., 2003)

abnormal locomotor rhythm, with Tsf3^KK109853

(Mandilaras and Missirlis, 2012)

abnormal neuroanatomy, with p38b^ala.UAS

(Vrailas-Mortimer et al., 2014)

abnormal neuroanatomy, with p38b^{kd.UAS.Tag:FLAG}

(Vrailas-Mortimer et al., 2014)

abnormal neuroanatomy, with p38b^UAS.cAa

(Vrailas-Mortimer et al., 2014)

abnormal neurophysiology | adult stage | conditional, with cry⁰¹, cry^{W397Y.UAS.EGFP}

(Baik et al., 2019)

abnormal neurophysiology | adult stage | conditional, with cry⁰¹, cry^{W420Y.UAS.EGFP}

(Baik et al., 2019)

abnormal phototaxis, with Hsap\KCNJ2^UAS.EGFP

(Keene et al., 2011)

abnormal sleep, with wake^RNAi.UAS.1

(Liu et al., 2014)

abnormal sleep | adult stage | female, with dyw^HMC04428

(Yang and Edery, 2019)

abnormal sleep | adult stage | male, with dyw^HMC04428

(Yang and Edery, 2019)

abnormal sleep | adult stage | male, with dyw^UAS.cYa

(Yang and Edery, 2019)

lethal, with Alas^KK101189

(Mandilaras and Missirlis, 2012)

lethal, with Eip75B^GD1434

(Mandilaras and Missirlis, 2012)

lethal, with Nlg4^GD3083

(Li et al., 2013)

viable, with Clk^UAS.cZa

(Zhao et al., 2003)

Phenotype Manifest In

l-LNv neuron | conditional, with cry⁰¹, cry^{W397Y.UAS.EGFP}

(Baik et al., 2019)

l-LNv neuron | conditional, with cry⁰¹, cry^{W420Y.UAS.EGFP}

(Baik et al., 2019)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^cry.PZ, p38b^ala.UAS has abnormal circadian rhythm phenotype, enhanceable by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

Scer\GAL4^cry.PZ, p38b^ala.UAS has abnormal neuroanatomy phenotype, enhanceable by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

Scer\GAL4^cry.PZ, p38b^{kd.UAS.Tag:FLAG} has abnormal circadian rhythm phenotype, enhanceable by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

Scer\GAL4^cry.PZ, p38b^{kd.UAS.Tag:FLAG} has abnormal neuroanatomy phenotype, enhanceable by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

NOT Enhanced by

Statement

Reference

Scer\GAL4^cry.PZ, p38b^DD.UAS has abnormal circadian rhythm phenotype, non-enhanceable by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

Clk^UAS.cZa, Scer\GAL4^cry.PZ has abnormal circadian rhythm phenotype, non-enhanceable by Pdf⁰¹

(Zhao et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^cry.PZ, p38b^UAS.cAa has abnormal circadian rhythm phenotype, suppressible by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

Scer\GAL4^cry.PZ, p38b^UAS.cAa has abnormal neuroanatomy phenotype, suppressible by Mef2^EnR.UAS, Scer\GAL4^cry.PZ

(Vrailas-Mortimer et al., 2014)

Mef2^EnR.UAS, Scer\GAL4^cry.PZ has abnormal circadian rhythm phenotype, suppressible by p38b^UAS.cAa, Scer\GAL4^cry.PZ

(Vrailas-Mortimer et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^cry.PZ, p38b^DD.UAS has abnormal circadian rhythm phenotype, non-suppressible by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

Clk^UAS.cZa, Scer\GAL4^cry.PZ has abnormal circadian rhythm phenotype, non-suppressible by Pdf⁰¹

(Zhao et al., 2003)

Suppressor of

Statement

Reference

Mef2^EnR.UAS, Scer\GAL4^cry.PZ is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^cry.PZ, p38b^UAS.cAa

(Vrailas-Mortimer et al., 2014)

p38b^UAS.cAa, Scer\GAL4^cry.PZ is a suppressor of abnormal circadian rhythm phenotype of Mef2^EnR.UAS, Scer\GAL4^cry.PZ

(Vrailas-Mortimer et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

cry^{W342Y.UAS.EGFP}/Scer\GAL4^cry.PZ rescues cry⁰¹

(Baik et al., 2019)

Partially rescues

cry^{W420Y.UAS.EGFP}/Scer\GAL4^cry.PZ partially rescues cry⁰¹

(Baik et al., 2019)

Scer\GAL4^cry.PZ/cry^{W397Y.UAS.EGFP} partially rescues cry⁰¹

(Baik et al., 2019)

Scer\GAL4^cry.PZ/Pdfr^UAS.cMa partially rescues Pdfr³³⁶⁹

(Im and Taghert, 2010)

Scer\GAL4^cry.PZ/na^UAS.cLa partially rescues na^har

(Lear et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

24514

y¹ w^*; P{cry-GAL4.Z}16

24774

P{cry-GAL4.Z}24, y¹ w^*

80940

P{cry-GAL4.Z}24, w^*; P{Pdf-GAL80.S}96A/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\GAL4^cry.PP

Scer\GAL4^cry.PZ

Name Synonyms

cryptochrome promoter construct of Zhao

Secondary FlyBase IDs

References (26)

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