FlyBase Allele Report: Dmel\Pdf[UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)]

General Information

Symbol

Dmel\Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}

Species

D. melanogaster

Name

FlyBase ID

FBal0285822

Feature type

allele

Associated gene

Dmel\Pdf

Associated Insertion(s)

Carried in Construct

P{UAS-Pdf.t.ML}

Also Known As

UAS-t-PDF, UAS-t-Pdf-ML

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Choi et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Pdf.t.ML}

Cytology

Description

UAS regulates expression of a 't-peptide' comprising (from N to C terminus): a trypsin secretory signal sequence for targeting to the secretory pathway, a mature cleaved Pdf peptide sequence, a medium length hydrophilic linker sequence of 14 amino acids with an embedded Tag:MYC epitope tag, and a GPI targeting sequence from lynx1 protoxin.

(Choi et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pdf

Tagged with

Tag:GPI(lynx1)

plasma membrane protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm, with Scer\GAL4^cry.PZ

(Choi et al., 2009)

abnormal locomotor rhythm, with Scer\GAL4^tim.UAS

(Choi et al., 2009)

abnormal locomotor rhythm, with Scer\GAL4^tim.UAS, Scer\GAL80^Pdf.PS

(Choi et al., 2009)

abnormal sleep, with Scer\GAL4^GMR18H11

(Kunst et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} under the control of Scer\GAL4^GMR18H11 significantly suppresses sleep late at night. This effect is blocked if the flies are also carrying Scer\GAL80^cry.PS.

Flies expressing Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} under the control of Scer\GAL4^nSyb.PU have free-running circadian rhythms with a strength and period indistinguishable from controls.

(Kunst et al., 2014)

Males expressing Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} under the control of Scer\GAL4^desat1.PB show a significant increase in the levels of (z)-7-Tricosene and (z)-7-Pentacosene at all circadian time points compared to wild type (measured on the sixth day of constant darkness).

(Krupp et al., 2013)

Scer\GAL4^tim.Scer\UAS-mediated expression of Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} induces complex free-running circadian locomotor rhythms in flies in constant darkness.

Scer\GAL4^Mz1366- or Scer\GAL4^Mz1525-mediated expression of Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} fails to induce free-running circadian locomotor rhythms in flies in constant darkness.

Scer\GAL4^tim.Scer\UAS-mediated expression of Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} in the presence of Scer\GAL80^Pdf.PS (i.e. expression in all clock neurons other than the Pdf-expressing LN[[V]]s) induces complex free-running circadian locomotor rhythms in flies in constant darkness.

Scer\GAL4^cry.PZ (P{cry-GAL4.Z}24)-mediated expression of Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} induces complex free-running circadian locomotor rhythms, with a short period of 22-23 hr and a long period of 25-26 hr, in flies in constant darkness. In contrast, Scer\GAL4^cry.PZ (P{cry-GAL4.Z}16)-mediated expression of Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} induces only a modest degree of complex rhythmicity and rather induced dramatic period shortening of 3-4 hr.

(Choi et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}, Scer\GAL4^GMR18H11 has abnormal sleep phenotype, suppressible | partially by Dh31^KG09001/Dh31^KG09001

(Kunst et al., 2014)

Suppressor of

Statement

Reference

Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}, Scer\GAL4^tim.PE is a suppressor | partially of abnormal locomotor rhythm phenotype of Dcr-2^UAS.cDa, Scer\GAL4^tim.PE, gw^KK101472

(Zhang and Emery, 2013)

Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}, Scer\GAL4^tim.PE is a suppressor | partially of abnormal locomotor rhythm phenotype of Dcr-2^UAS.cDa, Scer\GAL4^tim.PE, gw^HMS00105

(Zhang and Emery, 2013)

NOT Suppressor of

Statement

Reference

Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}/Scer\GAL4^tim.UAS is a non-suppressor of abnormal temperature response | adult stage phenotype of Dh31⁵¹, Pdf⁰¹

(Goda et al., 2018)

Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}/Scer\GAL4^P2.4.Pdf is a non-suppressor of abnormal temperature response | adult stage phenotype of Dh31⁵¹, Pdf⁰¹

(Goda et al., 2018)

Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}/Scer\GAL4^GMR18H11 is a non-suppressor of abnormal temperature response | adult stage phenotype of Dh31⁵¹, Pdf⁰¹

(Goda et al., 2018)

Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}, Scer\GAL4^Clk.int1-3, Scer\GAL80^Pdf.PS is a non-suppressor of abnormal temperature response | adult stage phenotype of Dh31⁵¹, Pdf⁰¹

(Goda et al., 2018)

Scer\GAL4^NP6301/Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)} is a non-suppressor of abnormal temperature response | adult stage phenotype of Dh31⁵¹, Pdf⁰¹

(Goda et al., 2018)

Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}, Scer\GAL4^Clk.int1-3, Scer\GAL80^Pdf.PS is a non-suppressor of abnormal temperature response | adult stage phenotype of Dh31⁵¹

(Goda et al., 2016)

Scer\GAL4^Pdf.PU/Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)} is a non-suppressor of abnormal temperature response | adult stage phenotype of Dh31⁵¹

(Goda et al., 2016)

Scer\GAL4^Clk.1.5/Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)} is a non-suppressor of abnormal temperature response | adult stage phenotype of Dh31⁵¹

(Goda et al., 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} under either the combined control of Scer\GAL4^Clk.int1-3 and Scer\GAL80^Pdf.PS, or the control of either Scer\GAL4^Pdf.PU, Scer\GAL4^Clk.4.1M or Scer\GAL4^Clk.4.5F, does not rescue the lower night-onset preferred temperature decrease observed in Dh31⁵¹ homozygous mutant adults.

(Goda et al., 2016)

The suppression of total nighttime sleep caused by expression of Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} under the control of Scer\GAL4^GMR18H11 is abolished in a Dh31^KG09001 homozygous background, although there remains a small, statistically significant decrease in sleep during the second half of the night.

(Kunst et al., 2014)

Pdfr⁵³⁰⁴ does not suppress the increase in the levels of (z)-7-Tricosene and (z)-7-Pentacosene compared to wild type seen in males expressing Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} under the control of Scer\GAL4^desat1.PB (measured on the sixth day of constant darkness).

(Krupp et al., 2013)

Co-expression of Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} rescues rhythmic locomotor behaviour under constant darkness conditions in 67.5% of flies expressing gw^KK101472 under the control of Scer\GAL4^tim.PE (in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency). The locomotor activity defects seen under 12 hour light/12 hour dark conditions in flies expressing gw^KK101472 under the control of Scer\GAL4^tim.PE (in the presence of Dcr-2^Scer\UAS.cDa) are not suppressed by co-expression of Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1}.

Co-expression of Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} rescues rhythmic locomotor behaviour under constant darkness conditions in 41.0% of flies expressing gw^HMS00105 under the control of Scer\GAL4^tim.PE (in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency).

(Zhang and Emery, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}/Scer\GAL4^tim.UAS partially rescues Pdf⁰¹

(Choi et al., 2009)

Comments

Pdf⁰¹ flies that also express Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} under the control of Scer\GAL4^tim.Scer\UAS exhibit little arrhythmicity in constant darkness (unlike Pdf⁰¹ only flies), with only ~10% of flies arrhythmic and most exhibiting instead a complex free-running rhythm (which is still abnormal compared to wild type).

(Choi et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

81110

w^*; P{UAS-Pdf.t.ML}F1a P{UAS-Pdf.t.ML}F5a

81111

w^*; P{UAS-Pdf.t.ML}M2a P{UAS-Pdf.t.ML}M3a; P{UAS-Pdf.t.ML}F1a P{UAS-Pdf.t.ML}F5a

Notes on Origin

Discoverer