FlyBase Allele Report: Scer\GAL4[Clk.int1-3]

General Information

Symbol

Scer\GAL4^Clk.int1-3

Species

S. cerevisiae

Name

FlyBase ID

FBal0279579

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Clk-GAL4.int1-3}

Also Known As

Clk9M-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Kaneko et al., 2012)

Progenitor genotype

Carried in construct

P{Clk-GAL4.int1-3}

Cytology

Description

A ~3kb fragment of Clk sequence, encompassing the first three Clk introns is cloned upstream of sequence encoding a GAL4 driver

(Kaneko et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

Clk

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

DN2 neuron

(Kaneko et al., 2012)

s-LNv Pdf neuron

(Kaneko et al., 2012)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Clk

(Kaneko et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm | adult stage | conditional, with Ctet\tetX^TNT-LC.UAS, Scer\GAL80^Pdf.PS

(Chen et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Elimination of molecular clock function (using cyc^{Δ.UAS.Tag:MYC}) in a subset of Scer\GAL4^Clk.int1-3 cells (restricted using Scer\GAL80^Pdf.PS) in adults does not affect the strength of the circadian rhythm of sleep-wake cycles, compared to controls.

(Bulthuis et al., 2019)

Inhibition (using Ctet\tetX^TNT-LC.UAS) of a subset of Scer\GAL4^Clk.int1-3 cells (restricted using Scer\GAL80^Pdf.PS) in adults results in a significantly lower circadian clock entrainment index for locomotor activity rhythms under 25[o] C:27 [o]C temperature cycles in constant light conditions, compared to controls; this inhibition does not significantly affect the phase-shift, compared to controls, when light-dark entrained flies are moved to dark-dark with 8 h delayed 25[o] C:27 [o]C temperature cycles.

(Chen et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}, Scer\GAL4^Clk.int1-3, Scer\GAL80^Pdf.PS is a non-suppressor of abnormal temperature response | adult stage phenotype of Dh31⁵¹, Pdf⁰¹

(Goda et al., 2018)

Dh31^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}, Scer\GAL4^Clk.int1-3, Scer\GAL80^Pdf.PS is a non-suppressor of abnormal temperature response | adult stage phenotype of Dh31⁵¹, Pdf⁰¹