FlyBase Allele Report: Dmel\Pdfr[UAS.cMa]

General Information

Symbol

Dmel\Pdfr^UAS.cMa

Species

D. melanogaster

Name

FlyBase ID

FBal0218036

Feature type

allele

Associated gene

Dmel\Pdfr

Associated Insertion(s)

Carried in Construct

P{UAS-pdfr.M}

Also Known As

UAS-Pdfr, UAS-pdfr-16

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Mertens et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-pdfr.M}

Cytology

Description

The pdfr open reading frame (2010bp), derived from the RH51348 EST sequence, plus an additional 108bp exon at position 424, is cloned into pPUAST.

(Mertens et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pdfr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pdfr^UAS.cMa, Scer\GAL4^P2.4.Pdf is a suppressor | partially of abnormal locomotor rhythm phenotype of Ac3^UAS.cDa, Scer\GAL4^P2.4.Pdf

(Duvall and Taghert, 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Pdfr^Scer\UAS.cMa fully rescues the response of the M pacemaker neurons in the brain to Pdf to wild-type levels in flies expressing Ac3^Scer\UAS.cDa under the control of Scer\GAL4^P2.4.Pdf.

Co-expression of Pdfr^Scer\UAS.cMa fully rescues the response of the M pacemaker neurons in the brain to Pdf to wild-type levels in flies expressing G-sα60A^Scer\UAS.cCa under the control of Scer\GAL4^P2.4.Pdf.

(Duvall and Taghert, 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Partially rescues

Scer\GAL4^Pdfr.B/Pdfr^UAS.cMa partially rescues Pdfr³³⁶⁹

(Im and Taghert, 2010)

Scer\GAL4^cry.PE/Pdfr^UAS.cMa partially rescues Pdfr³³⁶⁹

(Im and Taghert, 2010)

Scer\GAL4^tim.PE/Pdfr^UAS.cMa partially rescues Pdfr³³⁶⁹

(Im and Taghert, 2010)

Scer\GAL4^dimm-929/Pdfr^UAS.cMa partially rescues Pdfr³³⁶⁹

(Im and Taghert, 2010)

Scer\GAL4^cry.PZ/Pdfr^UAS.cMa partially rescues Pdfr³³⁶⁹

(Im and Taghert, 2010)

Scer\GAL4^Clk.8.0/Pdfr^UAS.cMa partially rescues Pdfr⁵³⁰⁴

(Lear et al., 2009)

Scer\GAL4^cry.PE/Pdfr^UAS.cMa partially rescues Pdfr⁵³⁰⁴

(Lear et al., 2009)

Scer\GAL4^cry.PE, Pdfr^UAS.cMa, Scer\GAL80^Pdf.PS partially rescues Pdfr⁵³⁰⁴

(Lear et al., 2009)

Scer\GAL4^elav.PLu, Pdfr^UAS.cMa, Scer\GAL80^Pdf.PS partially rescues Pdfr⁵³⁰⁴

(Lear et al., 2009)

Fails to rescue

Scer\GAL4^Clk.1.5/Pdfr^UAS.cMa fails to rescue Pdfr⁵³⁰⁴

(Goda et al., 2016)

Scer\GAL4^Pdf.PU/Pdfr^UAS.cMa fails to rescue Pdfr⁵³⁰⁴

(Goda et al., 2016)

Scer\GAL4^P2.4.Pdf/Pdfr^UAS.cMa fails to rescue Pdfr⁵³⁰⁴

(Lear et al., 2009)

Comments

Expression of Pdfr^Scer\UAS.cMa under the combined control of Scer\GAL4^Clk.int1-3 and Scer\GAL80^Pdf.PS, but not under the control of either Scer\GAL4^Clk.4.5F or Scer\GAL4^Pdf.PU, rescues the lower night-onset preferred temperature decrease observed in Pdfr⁵³⁰⁴ homozygous mutant adults; Pdfr⁵³⁰⁴ homozygous mutant adults expressing Pdfr^Scer\UAS.cMa under the combined control of Scer\GAL4^Clk.int1-3 and Scer\GAL80^Pdf.PS also display significantly higher daytime preferred temperature increase compared to controls.

(Goda et al., 2016)

Expression of Pdfr^Scer\UAS.cMa in DN1[[p]]s under the control of Scer\GAL4^Clk.4.5F restores normal light-dependent temperature preference in Pdfr⁵³⁰⁴ mutants.

(Head et al., 2015)

The advanced evening peak of locomotor activity which is seen in Pdfr³³⁶⁹ flies under light-dark conditions is rescued by expression of Pdfr^Scer\UAS.cMa under the control of one of Scer\GAL4^Pdfr.B, Scer\GAL4^cry.PE, Scer\GAL4^tim.PE or Scer\GAL4^dimm-929, but is not rescued by expression of Pdfr^Scer\UAS.cMa under the control of one of Scer\GAL4^P2.4.Pdf, Scer\GAL4^cry.PZ or Scer\GAL4^Aph-4-c232.

Under constant darkness conditions (measured on days 3-9), expression of Pdfr^Scer\UAS.cMa under the control of Scer\GAL4^cry.PZ restores strong rhythmic behavior in Pdfr³³⁶⁹ flies, but the phase is delayed 6-10 hours. In approximately 50% of the rhythmic flies this delay happens instantaneously, whereas in others it takes 3-4 days to shift and then free-run with a period of 23.7 hours.

(Im and Taghert, 2010)

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^Clk.8.0 in a pdfr⁵³⁰⁴ background rescues the defects in morning anticipation and the timing of light-dark entrained evening behaviour.

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^cry.PE in a pdfr⁵³⁰⁴ background restores the timing of evening behaviour and also promotes significant restoration of morning behaviour during light-dark entrainment and the first day of constant dark.

Expression of pdfr^Scer\UAS.cMa only in "evening" cells using Scer\GAL4^cry.PE together with Scer\GAL80^Pdf.PS in a pdfr⁵³⁰⁴ background rescues both the timing of evening behaviour and the magnitude of morning anticipation during light-dark entrainment and the first day of constant dark.

Expression of pdfr^Scer\UAS.cMa only in "morning" cells using Scer\GAL4^P2.4.Pdf in a pdfr⁵³⁰⁴ background does not rescue the timing of either morning or evening behaviours during light-dark entrainment and the first day of constant dark.

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^Clk.8.0 in a pdfr⁵³⁰⁴ background promotes significant rescue of rhythmicity and period length in constant dark.

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^cry.PE in a pdfr⁵³⁰⁴ background rescues period length and partially restores rhythmicity in constant dark.

Expression of pdfr^Scer\UAS.cMa only in "evening" cells using Scer\GAL4^cry.PE together with Scer\GAL80^Pdf.PS in a pdfr⁵³⁰⁴ background does not rescue rhythmic power though does rescue period length.

Expression of pdfr^Scer\UAS.cMa only in "morning" cells using Scer\GAL4^P2.4.Pdf in a pdfr⁵³⁰⁴ background has no significant effect on either free-running rhythmicity or period length in constant darkness.

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^elav.PLu in a pdfr⁵³⁰⁴ background rescues all circadian rhythm phenotypes in both light-dark and constant dark conditions. Co-expression of Scer\GAL80^Pdf.PS results in a substantial reduction in rescue of rhythmic power in constant dark, though doesn't affect the rescue of period length in constant dark.

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^{Pdfr-EY11181-GAL4} in a pdfr⁵³⁰⁴/pdfr^EY11181-GAL4 background strongly rescues the reduced rhythmicity phenotype seen in constant dark conditions.

(Lear et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Pdfr^Scer\UAS.cMa

Pdfr^UAS.cMa

pdfr^Scer\UAS.cMa

Name Synonyms

Secondary FlyBase IDs

References (13)

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