FlyBase Allele Report: Dmel\Pdfr[5304]

General Information

Symbol

Dmel\Pdfr⁵³⁰⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0218040

Feature type

allele

Associated gene

Dmel\Pdfr

Associated Insertion(s)

Carried in Construct

Also Known As

han⁵³⁰⁴, pdfr^han5304, pdfr^han5403

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Hyun et al., 2005)

Progenitor genotype

Pdfr^X3026

(Hyun et al., 2005)

Cytology

Description

Mobilisation of P{EP}pdfr^X3026 results in a large deletion that excludes all of the transmembrane domains and the C-terminus of pdfr.

(Hyun et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate sleep disorder

modeled by Nf1^P1, Nf1^P2

(Bai et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult s-LNv neuron | adult stage

(Herrero et al., 2020)

Detailed Description

Statement

Reference

Pdfr⁵³⁰⁴ hemizygotes show severe and nearly sully penetrant circadian arrhythmicity and a significantly shorter circadian period.

(Bai et al., 2018)

Pdfr⁵³⁰⁴/Y mutant adults have significantly reduced strength of circadian locomotor rhythms under constant darkness, compared to heterozygous controls.

(King et al., 2017)

Pdfr⁵³⁰⁴ homozygous mutant adults, reared under 12:12 light:dark zeitgeber cycle, present a lower night-onset preferred temperature decrease (between zeitgeber times 10-12 and 13-15) but similar day-time preferred temperature increase, as compared to controls.

(Goda et al., 2016)

In contrast to wild-type, Pdfr⁵³⁰⁴ mutants fail to exhibit any light-dependent temperature preference.

(Head et al., 2015)

In constant darkness, almost a quarter of Pdfr⁵³⁰⁴/Pdfr⁵³⁰⁴ flies are arrhythmic and almost half have weak rhythms.

(Zheng et al., 2014)

Mutant flies show severely reduced morning anticipation and the phase of the evening peak of activity is advanced compared to controls when circadian locomotor activity is analysed under 12 hours light/12 hour dark conditions. The flies are mostly arrhythmic under constant darkness conditions.

(Zhang and Emery, 2013)

The E pacemaker neurons in the brain of mutant flies show no response to Pdf, in contrast to wild type.

(Duvall and Taghert, 2012)

Pdfr⁵³⁰⁴ mutants exhibit daytime temperature preference rhythms similar to those of wild-type flies during LD.

(Kaneko et al., 2012)

Mutant flies maintain a morning peak of circadian locomotor activity under 12 hour light:12 hour dark conditions, but show an advanced evening peak of activity compared to wild-type flies. 59.3% of the mutant flies are arrhythmic in constant darkness conditions (measured during days 3-9).

(Im and Taghert, 2010)

pdfr⁵³⁰⁴ mutants exhibit phase-advanced evening anticipation.

Behaviour rhythms of pdfr⁵³⁰⁴ mutants damp in DD and are accompanied by a shorter period.

(Zhang et al., 2010)

Under light:dark (LD) conditions, mutant flies show increases in sleep during the late night. At the time when wild-type flies are waking up in advance of lights-on, the mutant flies fail to wake up.

During the first day of constant darkness (DD) conditions, mutant flies show a significant increase in total sleep during the subjective day compared to control flies.

(Chung et al., 2009)

Homozygotes display strongly reduced morning anticipation and phase-advanced evening anticipation under conditions of light-dark entrainment and on the first day of constant darkness.

pdfr^EY11181-GAL4/pdfr⁵³⁰⁴ flies display poor rhythmicity in constant dark conditions.

(Lear et al., 2009)

pdfr⁵³⁰⁴ is viable and does not show any atypical morphological phenotype.

pdfr⁵³⁰⁴ flies appear to constantly prefer a temperature of 23.5^oC, regardless of whether it is day or night. This is a little colder than the temperature preferred by wild-type flies.

pdfr⁵³⁰⁴ mutants show reduced behavioural activity in the morning during LD cycles and during the first day of the DD cycle; the average activity during the first wo hours after 'light on' is 10% of the total daily activity in LD. More than 60% of pdfr⁵³⁰⁴ flies show less than 10% of their total daily activity during the first two hours in the morning. pdfr⁵³⁰⁴ flies do not demonstrate any gradual increase of actvity before lights are turned on, indicating that these mutants have a deficit in morning anticipation.

pdfr⁵³⁰⁴ mutants display an abnormality in evening behavioural activity. They begin evening activity earlier than wild-type flies and the activity continues until lights are turned off in LD cycles. The highest evening activities are observed at ZT9.6, compared to ZT11.0 in control flies, suggesting that evening anticipation is advanced in pdfr⁵³⁰⁴ mutants.

pdfr³³⁶⁹ mutant flies exhibit abnormal circadian rhythmic behaviour under DD conditions. They show residual circadian rhythmic behaviour in the first few days, but they gradually lose the rhythmic activity as the DD condition continues. The rhythmic power of the pdfr³³⁶⁹ mutant is 69 in DD, compared to 121 in control flies. pdfr³³⁶⁹ mutants demonstrate a shortened rhythmic period of 22.7 hours, compared to 23.3 hours in wild-type. The rhythmicity during D is observed in only 10-15% of individual flies tested in the pdfr³³⁶⁹ genotype.

(Hyun et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Pdfr⁵³⁰⁴ has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by TrpA1^UAS.(B).cKa/Scer\GAL4^Pdf.Switch1a

(Herrero et al., 2020)

Pdfr⁵³⁰⁴ has abnormal circadian rhythm | adult stage phenotype, non-enhanceable by TrpA1^UAS.(B).cKa/Scer\GAL4^Pdf.Switch1a

(Herrero et al., 2020)

Suppressed by

Statement

Reference

Pdfr⁵³⁰⁴ has abnormal locomotor rhythm phenotype, suppressible by dco^{AR.UAS.Tag:NES(Unk)}/Scer\GAL4^cry.PU

(Zheng et al., 2014)

NOT suppressed by

Statement

Reference

Pdfr⁵³⁰⁴ has abnormal neuroanatomy | adult stage phenotype, non-suppressible by TrpA1^UAS.(B).cKa/Scer\GAL4^Pdf.Switch1a

(Herrero et al., 2020)

Pdfr⁵³⁰⁴ has abnormal circadian rhythm | adult stage phenotype, non-suppressible by TrpA1^UAS.(B).cKa/Scer\GAL4^Pdf.Switch1a

(Herrero et al., 2020)

Pdfr⁵³⁰⁴ has abnormal locomotor rhythm phenotype, non-suppressible by dco^{UAS.Tag:NES(Unk)}/Scer\GAL4^cry.PU

(Zheng et al., 2014)

NOT Suppressor of

Statement

Reference

Pdfr[+]/Pdfr⁵³⁰⁴ is a non-suppressor of abnormal circadian rhythm | adult stage phenotype of Nf1^P2/Nf1^P1

(Bai et al., 2018)

Pdfr⁵³⁰⁴/Pdfr⁵³⁰⁴ is a non-suppressor of abnormal circadian rhythm | adult stage phenotype of Nf1^P2/Nf1^P1

(Bai et al., 2018)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Pdfr⁵³⁰⁴ has adult s-LNv neuron | adult stage phenotype, non-enhanceable by TrpA1^UAS.(B).cKa/Scer\GAL4^Pdf.Switch1a

(Herrero et al., 2020)

NOT suppressed by

Statement

Reference

Pdfr⁵³⁰⁴ has adult s-LNv neuron | adult stage phenotype, non-suppressible by TrpA1^UAS.(B).cKa/Scer\GAL4^Pdf.Switch1a

(Herrero et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Nf1^P1 heterozygosity suppresses the shorter circadian period of Pdfr⁵³⁰⁴ hemizygotes, but not their circadian arrhythmicity.

(Bai et al., 2018)

Driving dco^{AR.Scer\UAS.T:Uuuu\nes-Unk} but not dco^{Scer\UAS.T:Uuuu\nes-Unk} with Scer\GAL4^cry.PU partially restores rhythmicity to Pdfr⁵³⁰⁴/Pdfr⁵³⁰⁴ flies.

(Zheng et al., 2014)

Pdfr⁵³⁰⁴ does not suppress the increase in the levels of (z)-7-Tricosene and (z)-7-Pentacosene compared to wild type seen in males expressing Pdf^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} under the control of Scer\GAL4^desat1.PB (measured on the sixth day of constant darkness).

(Krupp et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Pdfr⁵³⁰⁴ is rescued by Scer\GAL4^Clk.int1-3/Scer\GAL80^Pdf.PS/Pdfr^UAS.cMa

(Goda et al., 2016)

Pdfr⁵³⁰⁴ is rescued by Scer\GAL4^Clk.1.5/Pdfr^UAS.cMa

(Head et al., 2015)

Pdfr⁵³⁰⁴ is rescued by Pdfr^Tag:MYC

(Im and Taghert, 2010)

Pdfr^EY11851-GAL4/Pdfr⁵³⁰⁴ is rescued by Scer\GAL4^{Pdfr-EY11851-GAL4}/Pdfr^UAS.cMa

(Lear et al., 2009)

Pdfr⁵³⁰⁴ is rescued by Scer\GAL4^Clk.8.0/Pdfr^UAS.cMa

(Lear et al., 2009)

Pdfr⁵³⁰⁴ is rescued by Scer\GAL4^cry.PE/Pdfr^UAS.cMa/Scer\GAL80^Pdf.PS

(Lear et al., 2009)

Pdfr⁵³⁰⁴ is rescued by Scer\GAL4^elav.PLu/Pdfr^UAS.cMa

(Lear et al., 2009)

Partially rescued by

Pdfr⁵³⁰⁴ is partially rescued by Scer\GAL4^Clk.8.0/Pdfr^UAS.cMa

(Lear et al., 2009)

Pdfr⁵³⁰⁴ is partially rescued by Scer\GAL4^cry.PE/Pdfr^UAS.cMa

(Lear et al., 2009)

Pdfr⁵³⁰⁴ is partially rescued by Scer\GAL4^cry.PE/Pdfr^UAS.cMa/Scer\GAL80^Pdf.PS

(Lear et al., 2009)

Pdfr⁵³⁰⁴ is partially rescued by Scer\GAL4^elav.PLu/Pdfr^UAS.cMa/Scer\GAL80^Pdf.PS

(Lear et al., 2009)

Not rescued by

Pdfr⁵³⁰⁴ is not rescued by Scer\GAL4^Clk.1.5/Pdfr^UAS.cMa

(Goda et al., 2016)

Pdfr⁵³⁰⁴ is not rescued by Scer\GAL4^Pdf.PU/Pdfr^UAS.cMa

(Goda et al., 2016)

Pdfr⁵³⁰⁴ is not rescued by Scer\GAL4^P2.4.Pdf/Pdfr^UAS.cMa

(Lear et al., 2009)

Comments

Expression of Pdfr^Scer\UAS.cMa under the combined control of Scer\GAL4^Clk.int1-3 and Scer\GAL80^Pdf.PS, but not under the control of either Scer\GAL4^Clk.4.5F or Scer\GAL4^Pdf.PU, rescues the lower night-onset preferred temperature decrease observed in Pdfr⁵³⁰⁴ homozygous mutant adults; expression under the combined control of Scer\GAL4^Clk.int1-3 and Scer\GAL80^Pdf.PS also significantly exacerbates the day-time preferred temperature increase observed in Pdfr⁵³⁰⁴ homozygous mutant adults.

(Goda et al., 2016)

Expression of Pdfr^Scer\UAS.cMa in DN1[[p]]s under the control of Scer\GAL4^Clk.4.5F restores normal light-dependent temperature preference in Pdfr⁵³⁰⁴ mutants.

(Head et al., 2015)

Pdfr^T:Hsap\MYC rescues both the advanced evening peak of locomotor activity which is seen in Pdfr⁵³⁰⁴ flies under light-dark conditions and the arrhythmicity of Pdfr⁵³⁰⁴ flies under constant darkness conditions.

(Im and Taghert, 2010)

The evening phase-advanced anticipation seen in pdfr⁵³⁰⁴ mutants is not rescued by expression of pdfr^Scer\UAS.cZa under the control of Scer\GAL4^Clk.4.5F or Scer\GAL4^Clk.4.1M. In contrast, morning anticipation is resuced by both Scer\GAL4^Clk.4.1M and Scer\GAL4^Clk.4.5F expression of pdfr^Scer\UAS.cZa.

Behaviour rhythms of pdfr⁵³⁰⁴ mutants are rescued by Scer\GAL4^Clk.4.1M-driven expression of pdfr^Scer\UAS.cZa. There is significant improvements of rhythm amplitude with Scer\GAL4^Clk.4.1M-driven expression of pdfr^Scer\UAS.cZa but not with Scer\GAL4^Clk.4.5F-driven expression.

(Zhang et al., 2010)

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^Clk.8.0 in a pdfr⁵³⁰⁴ background rescues the defects in morning anticipation and the timing of light-dark entrained evening behaviour.

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^cry.PE in a pdfr⁵³⁰⁴ background restores the timing of evening behaviour and also promotes significant restoration of morning behaviour during light-dark entrainment and the first day of constant dark.

Expression of pdfr^Scer\UAS.cMa only in "evening" cells using Scer\GAL4^cry.PE together with Scer\GAL80^Pdf.PS in a pdfr⁵³⁰⁴ background rescues both the timing of evening behaviour and the magnitude of morning anticipation during light-dark entrainment and the first day of constant dark.

Expression of pdfr^Scer\UAS.cMa only in "morning" cells using Scer\GAL4^P2.4.Pdf in a pdfr⁵³⁰⁴ background does not rescue the timing of either morning or evening behaviours during light-dark entrainment and the first day of constant dark.

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^Clk.8.0 in a pdfr⁵³⁰⁴ background promotes significant rescue of rhythmicity and period length in constant dark.

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^cry.PE in a pdfr⁵³⁰⁴ background rescues period length and partially restores rhythmicity in constant dark.

Expression of pdfr^Scer\UAS.cMa only in "evening" cells using Scer\GAL4^cry.PE together with Scer\GAL80^Pdf.PS in a pdfr⁵³⁰⁴ background does not rescue rhythmic power though does rescue period length.

Expression of pdfr^Scer\UAS.cMa only in "morning" cells using Scer\GAL4^P2.4.Pdf in a pdfr⁵³⁰⁴ background has no significant effect on either free-running rhythmicity or period length in constant darkness.

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^elav.PLu in a pdfr⁵³⁰⁴ background rescues all circadian rhythm phenotypes in both light-dark and constant dark conditions. Co-expression of Scer\GAL80^Pdf.PS results in a substantial reduction in rescue of rhythmic power in constant dark, though doesn't affect the rescue of period length in constant dark.

Expression of pdfr^Scer\UAS.cMa using Scer\GAL4^{Pdfr-EY11181-GAL4} in a pdfr⁵³⁰⁴/pdfr^EY11181-GAL4 background strongly rescues the reduced rhythmicity phenotype seen in constant dark conditions.

(Lear et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33068

Pdfr⁵³⁰⁴ w^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

Han⁵³⁰⁴

(Li et al., 2018)

PdfR⁵³⁰⁴

(Elya et al., 2023, Yao et al., 2012)

Pdfr⁵³⁰⁴

(Goda et al., 2019, Hajji et al., 2019, Goda et al., 2016, Means et al., 2015, Zheng et al., 2014, Krupp et al., 2013)

Pdfr^han5304

(Chatterjee et al., 2018, Luo and Sehgal, 2012, Zhang et al., 2010, Zhang et al., 2010, Lear et al., 2009)

han5304

(Zhang and Emery, 2013)

han³³⁶⁹

(Hyun et al., 2005)

han⁵³⁰⁴

(Johnstone et al., 2022, Li et al., 2022, Song et al., 2021, Herrero et al., 2020, Schlichting et al., 2019, Chen et al., 2016, Duvall and Taghert, 2012, Beckwith et al., 2011, Im and Taghert, 2010, Picot et al., 2009, Shafer et al., 2008)

pdfR^Han5

(Mezan et al., 2016)

pdfr⁵³⁰⁴

(Sabado et al., 2017, Head et al., 2015, Guo et al., 2014, Im et al., 2011, Im and Taghert, 2010)

pdfr^Han5304

(Kaneko et al., 2012)

pdfr^KO

(Means et al., 2015)

pdfr^han5304

(King et al., 2017, Chung et al., 2009)

pdfr^han5403

(Liang et al., 2023, Liang et al., 2019, Liang et al., 2017)

pdfr^han

(Li et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (39)

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