PdfrT:HsapMYC is expressed throughout the brain, and in some cases in close proximity to the projections of clock neurons or in the neurons themselves. PdfrT:HsapMYC is strongly expressed in the Pdf negative s-LNV neuron, three LNd's, and six DN1 neurons. It is weakly expressed in the s-LNv Pdf neurons and its dorsal projections, in two l-LNv's and its projections in the posterior optic commissure, and around four DN1's, DN2's and 10 DN3 neurons. PdfrT:HsapMYC also labels the boundary between the retina and the lamina and around 10 cells and its projections in the tritocerebrum and subesophageal ganglion. These cells are in close proximity to the processes of the Pdf-positive neurons of the tritocerebrum.
Co-expression of PdfrT:Hsap\MYC partially rescues the response of the M pacemaker neurons in the brain to Pdf to wild-type levels in flies expressing Ac3Scer\UAS.cDa under the control of Scer\GAL4P2.4.Pdf.
PdfrTag:MYC rescues Pdfr3369
PdfrTag:MYC rescues Pdfr5304
PdfrT:Hsap\MYC rescues both the advanced evening peak of locomotor activity which is seen in Pdfr3369 and Pdfr5304 flies under light-dark conditions and the arrhythmicity of Pdfr3369 and Pdfr5304 flies under constant darkness conditions.
