FlyBase Allele Report: Dmel\Dh31[UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)]

General Information

Symbol

Dmel\Dh31^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}

Species

D. melanogaster

Name

FlyBase ID

FBal0285826

Feature type

allele

Associated gene

Dmel\Dh31

Associated Insertion(s)

Carried in Construct

P{UAS-Dh31.t.ML}

Also Known As

UAS-t-DH31

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Choi et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Dh31.t.ML}

Cytology

Description

UAS regulates expression of a 't-peptide' comprising (from N to C terminus): a trypsin secretory signal sequence for targeting to the secretory pathway, a mature cleaved Dh31 peptide sequence, a medium length hydrophilic linker sequence of 14 amino acids with an embedded Tag:MYC epitope tag, and a GPI targeting sequence from lynx1 protoxin.

(Choi et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Dh31

Tagged with

Tag:GPI(lynx1)

plasma membrane protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^nSyb.PU

(Kunst et al., 2014)

abnormal sleep, with Scer\GAL4^nSyb.PU

(Kunst et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Dh31^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} under the control of Scer\GAL4^nSyb.PU significantly suppresses nighttime sleep, with the effect being strongest during the second half of the night. The nighttime sleep is less consolidated than that of controls, with the mutant flies showing more sleep bouts of shorter duration.

Expression of Dh31^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} under the control of any one of Scer\GAL4^GMR18H11, Scer\GAL4^GMR43D05, Scer\GAL4^GMR61G12, Scer\GAL4^GMR23D06, Scer\GAL4^GMR23F10, Scer\GAL4^GMR23H01, Scer\GAL4^GMR23H02, Scer\GAL4^GMR24D10, Scer\GAL4^GMR24D11, Scer\GAL4^GMR24E02, Scer\GAL4^GMR24G08, Scer\GAL4^GMR24H08, Scer\GAL4^GMR25A08, Scer\GAL4^GMR28D01, Scer\GAL4^GMR38B06, Scer\GAL4^GMR84C10, Scer\GAL4^GMR10D10, Scer\GAL4^GMR10G07, Scer\GAL4^GMR16B04, Scer\GAL4^GMR19A11, Scer\GAL4^GMR34G04, Scer\GAL4^GMR40F04, Scer\GAL4^GMR51F10, Scer\GAL4^GMR54D01, Scer\GAL4^GMR67C01, Scer\GAL4^Cha.7.4, Scer\GAL4^Ddc.PL, Scer\GAL4^Gad1.3.098, Scer\GAL4^VGlut-OK371, Scer\GAL4^Tdc2.PC, Scer\GAL4^ple.PF, Scer\GAL4^Trh.PS or Scer\GAL4^tim.Scer\UAS has no effect on sleep.

Expression of Dh31^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} under the control of Scer\GAL4^nSyb.PU has no effect on the strength of have free-running circadian rhythms, but results in a small decrease in the free-running period.

(Kunst et al., 2014)

Scer\GAL4^tim.Scer\UAS-mediated expression of Dh31^{Scer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1} fails to induce free-running circadian locomotor rhythms in flies in constant darkness.

(Choi et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^tim.UAS/Dh31^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)} is a suppressor | partially of abnormal temperature response | adult stage phenotype of Dh31⁵¹, Pdf⁰¹