FB2024_03 , released June 25, 2024
Allele: Dmel\PdfUAS.SCR.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)
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General Information
Symbol
Dmel\PdfUAS.SCR.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)
Species
D. melanogaster
Name
FlyBase ID
FBal0285825
Feature type
allele
Associated gene
Dmel\Pdf
Associated Insertion(s)
Carried in Construct
P{UAS-Pdf.t.SCR}
Also Known As
UAS-t-PDF-SCR
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Choi et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-Pdf.t.SCR}
Cytology
Description

UAS regulates expression of a 't-peptide' comprising (from N to C terminus): a trypsin secretory signal sequence for targeting to the secretory pathway, a scrambled Pdf peptide sequence, a medium length hydrophilic linker sequence of 14 amino acids with an embedded Tag:MYC epitope tag, and a GPI targeting sequence from lynx1 protoxin.

(Choi et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Pdf
Tagged with
Tag:GPI(lynx1)
Tag:MYC
Tag:SS(trypsin)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4tim.Scer\UAS-mediated expression of PdfScer\UAS.SCR.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1 fails to induce free-running circadian locomotor rhythms in flies in constant darkness.

      Scer\GAL4cry.PZ-mediated expression of PdfScer\UAS.SCR.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1 fails to induce free-running circadian locomotor rhythms in flies in constant darkness.

      (Choi et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of PdfScer\UAS.SCR.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1 under either the combined control of Scer\GAL4Clk.int1-3 and Scer\GAL80Pdf.PS, or the control of either Scer\GAL4Pdf.PU, Scer\GAL4Clk.4.1M or Scer\GAL4Clk.4.5F, does not rescue the lower night-onset preferred temperature decrease observed in Dh3151 homozygous mutant adults.

      (Goda et al., 2016)

      Co-expression of PdfScer\UAS.SCR.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1 does not rescue rhythmic locomotor behaviour under constant darkness conditions in flies expressing gwKK101472 under the control of Scer\GAL4tim.PE (in the presence of Dcr-2Scer\UAS.cDa).

      (Zhang and Emery, 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      PdfScer\UAS.SCR.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1
      PdfUAS.SCR.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)