FlyBase Allele Report: Dmel\Fer2LCH[KK103577]

General Information

Symbol

Dmel\Fer2LCH^KK103577

Species

D. melanogaster

Name

FlyBase ID

FBal0232401

Feature type

allele

Associated gene

Dmel\Fer2LCH

Associated Insertion(s)

Carried in Construct

P{KK103577}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK103577}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK103577

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression via P{GAL4-tim.E}27 or P{cry-GAL4.Z}17 (in the presence of Dcr-2^Scer\UAS.cDa) results in robust reduction in the the number of flies showing rhythmic behaviour in constant darkness and a concomitant reduction in the 'rhythmic strength' value of flies classified as rhythmic. Inclusion of Scer\GAL80^cry.PS rescues the circadian rhythm defects of P{GAL4-tim.E}27 Fer2LCH^KK103577 flies; inclusion of Scer\GAL80^Pdf.PS partially rescues these defects.

Under 12:12 hour alternating light/dark cycles, control and P{GAL4-tim.E}27 Fer2LCH^KK103577 Dcr-2^Scer\UAS.cDa flies show no apparent differences in locomotor circadian rhythm. Rhythm breaks down under constant darkness conditions, and is restored upon re-establishment of light/dark cycles. Clock neurons are not killed by Fer2LCH^KK103577 expression.

Circadian arrhythmicity in constant darkness is observed when when Fer2LCH^KK103577 (together with Dcr-2^Scer\UAS.cDa) is expressed via Scer\GAL4^Mai179, P{GAL4-tim.E}62, P{GAL4-tim.E}82, P{GAL4-tim.E}86, P{cry-GAL4.E}39 or Scer\GAL4^elav-C155.

Circadian behaviour in constant darkness is not affected when Fer2LCH^KK103577 (together with Dcr-2^Scer\UAS.cDa) is expressed via Scer\GAL4^P2.4.Pdf, P{cry-GAL4.E}19, Scer\GAL4^Clk.4.1M, Scer\GAL4^Clk.4.5F, Scer\GAL4^nrv2.PS, Scer\GAL4^C805, Scer\GAL4^NP3084, Scer\GAL4^GMR.PFa or Scer\GAL4^ple.PF.