FlyBase Allele Report: Dmel\na[UAS.cLa]

General Information

Symbol

Dmel\na^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Lear

FlyBase ID

FBal0244030

Feature type

allele

Associated gene

Dmel\na

Associated Insertion(s)

Carried in Construct

P{UAS-na.L}

Also Known As

UAS-na

Key Links

Transgenic product class

cDNA

(Lear et al., 2005)

wild_type

(Lear et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Lear et al., 2005)

wild_type

(Lear et al., 2005)

Mutagen

in vitro construct

(Lear et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-na.L}

Cytology

Description

UAS regulatory sequences drive expression of a na cDNA.

(Lear et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian behavior, with Scer\GAL4^Clk.1.5

(Zhang et al., 2010)

abnormal locomotor rhythm, with Scer\GAL4^cry.PZ

(Lear et al., 2005)

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf

(Lear et al., 2005)

abnormal locomotor rhythm, with Scer\GAL4^tim.PE

(Lear et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of na^Scer\UAS.cLa under the control of Scer\GAL4^Clk.1.5 does not alter the acute responsiveness to light-on (the acute masking index) in assays of circadian locomotor activity.

(Zhang et al., 2010)

The acute masking index, which reflects the magnitude and direction of the activity response immediately following lights-on, along with morning anticipatory behaviour, are not changed upon expression of na^Scer\UAS.cLa in DN[[p]] neurons under the control of Scer\GAL4^Clk.4.1M.

(Zhang et al., 2010)

Expression of na^Scer\UAS.cLa under the control of either Scer\GAL4^P2.4.Pdf, Scer\GAL4^tim.PE or Scer\GAL4^cry.PZ results in an increase in the period length of circadian locomotor activity under constant darkness conditions.

(Lear et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Scer\GAL4^tim.PE/na^UAS.cLa is a non-suppressor of abnormal circadian behavior phenotype of unc79^x25

(Lear et al., 2013)

Scer\GAL4^tim.PE, unc80^UAS.Tag:HA, na^UAS.cLa is a non-suppressor of abnormal circadian behavior phenotype of unc79^x25

(Lear et al., 2013)

Scer\GAL4^tim.PE/na^UAS.cLa is a non-suppressor of abnormal circadian behavior phenotype of unc80^x42

(Lear et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of na^Scer\UAS.cLa under the control of Scer\GAL4^tim.PE does not suppress the circadian behaviour defects seen in unc79^x25 mutant flies.

Co-expression of na^Scer\UAS.cLa and unc80^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^tim.PE does not suppress the circadian behaviour defects seen in unc79^x25 mutant flies.

Expression of na^Scer\UAS.cLa under the control of Scer\GAL4^tim.PE does not suppress the circadian behaviour defects seen in unc80^x42 mutant flies.

(Lear et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Clk.1.5/na^UAS.cLa rescues na^har

(Zhang et al., 2010)

Scer\GAL4^elav-C155/na^UAS.cLa rescues na^har

(Lear et al., 2005)

na^UAS.cLa/Scer\GAL4^Clk.8.0 rescues na^har

(Lear et al., 2005)

Scer\GAL4^tim.PE/na^UAS.cLa rescues na^har

(Lear et al., 2005)

Partially rescues

Scer\GAL4^Clk.1.5/na^UAS.cLa partially rescues na^har

(Zhang et al., 2010)

Scer\GAL4^cry.PZ/na^UAS.cLa partially rescues na^har

(Lear et al., 2005)

na^UAS.cLa/Scer\GAL4^cry.PE partially rescues na^har

(Lear et al., 2005)

na^UAS.cLa/Scer\GAL4^Mai179 partially rescues na^har

(Lear et al., 2005)

Fails to rescue

na^UAS.cLa/Scer\GAL4^P2.4.Pdf fails to rescue na^har

(Lear et al., 2005)

Scer\GAL4¹¹¹⁸/na^UAS.cLa fails to rescue na^har

(Lear et al., 2005)

Comments

DN1[[P] rescue of na^har mutants through expression of na^Scer\UAS.cLa under the control of Scer\GAL4^Clk.4.1M restores only the acute responsiveness to lights-on (AMI), but not the sustained masking index.

Morning anticipatory behaviour is restored in na^har mutants where na^Scer\UAS.cLa is expressed under the control of Scer\GAL4^Clk.4.1M. However, evening behaviour under LD conditions is not rescued.

(Zhang et al., 2010)

Expression of na^Scer\UAS.cLa under the control of either Scer\GAL4^elav-C155, Scer\GAL4^Clk.8.0 or Scer\GAL4^tim.PE results in strong rescue of the circadian locomotor activity defects seen in na^har flies under 12 hour light/12 hour dark conditions, with rescue of evening anticipation, timing of morning anticipation and response to lights on.

Expression of na^Scer\UAS.cLa under the control of Scer\GAL4^cry.PZ results in robust rescue of the circadian locomotor activity defects seen in na^har flies under 12 hour light/12 hour dark conditions.

Expression of na^Scer\UAS.cLa under the control of either Scer\GAL4^P2.4.Pdf or Scer\GAL4¹¹¹⁸ fails to rescue the circadian locomotor activity defects seen in na^har flies under 12 hour light/12 hour dark conditions.

The rescue of the circadian locomotor activity defects seen in na^har flies under 12 hour light/12 hour dark conditions by expression of na^Scer\UAS.cLa under the control of Scer\GAL4^elav-C155 is almost completely blocked if the flies are also expressing Scer\GAL80^cry.PS.

The rescue of the circadian locomotor activity defects seen in na^har flies under 12 hour light/12 hour dark conditions by expression of na^Scer\UAS.cLa under the control of either Scer\GAL4^elav-C155, Scer\GAL4^Clk.8.0 or Scer\GAL4^tim.PE is not strongly affected in the flies are also expressing Scer\GAL80^Pdf.PS.

Expression of na^Scer\UAS.cLa under the control of either Scer\GAL4^cry.PE or Scer\GAL4^Mai179 partially rescues the circadian locomotor activity defects seen in na^har flies under 12 hour light/12 hour dark conditions; evening anticipation is rescued, but the lights-on activity response is not rescued. Morning anticipation is not affected if expression is driven by Scer\GAL4^Mai179, but if expression is driven by Scer\GAL4^cry.PE the degree of morning anticipation is suppressed compared to na^har mutant controls. Evening anticipation is still rescued in na^har expressing na^Scer\UAS.cLa under the control of Scer\GAL4^cry.PE that are also expressing Scer\GAL80^Pdf.PS.

Expression of na^Scer\UAS.cLa under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^Clk.8.0 significantly rescues the circadian locomotor activity defects seen in na^har flies under constant darkness conditions, with a rescue of both morning and evening peaks being seen.

Expression of na^Scer\UAS.cLa under the control of Scer\GAL4^cry.PE partially rescues the circadian locomotor activity defects seen in na^har flies under constant darkness conditions; rescue of a single broad evening peak is seen, but there is either no morning anticipatory behaviour or it is very delayed and there are no reductions in midday activity compared to na^har mutant controls.

Expression of na^Scer\UAS.cLa under the control of Scer\GAL4^Mai179 partially rescues the circadian locomotor activity defects seen in na^har flies under constant darkness conditions; there is a strong rescue of the evening activity peak and a reduction in midday activity, but there is no change in morning anticipatory behaviour compared to na^har mutant controls.

Expression of na^Scer\UAS.cLa under the control of Scer\GAL4^tim.PE significantly rescues the circadian locomotor activity defects seen in na^har flies under constant darkness conditions.

Expression of na^Scer\UAS.cLa under the control of Scer\GAL4^cry.PZ weakly rescues the circadian locomotor activity defects seen in na^har flies under constant darkness conditions; a higher proportion of flies with rhythmic behaviour is seen and the rescued flies show clear persistant morning and evening peaks.

Expression of na^Scer\UAS.cLa under the control of Scer\GAL4^P2.4.Pdf fails to rescue the circadian locomotor activity defects seen in na^har flies under constant darkness conditions.

The rescue of the circadian locomotor activity defects seen in na^har flies under constant darkness conditions by expression of na^Scer\UAS.cLa under the control of either Scer\GAL4^elav-C155, Scer\GAL4^Clk.8.0, Scer\GAL4^cry.PE or Scer\GAL4^tim.PE is reduced if the flies are also expressing Scer\GAL80^Pdf.PS.

(Lear et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

na^Scer\UAS.cLa

na^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Lear

Secondary FlyBase IDs

References (6)

Report Sections

Open Close