UASt regulatory sequences drive expression of an inverted repeat.
The expression of IscUGD14694 under the control of Scer\GAL4cry.PZ leads to a decrease in circadian rhythmicity of adults transfered to constant darkness conditions, as compared to wild-type controls (or to 12h:12h light:dark cycles). Expression under the control of Scer\GAL4tim.PE leads to lethality.
Expression with P{GAL4-tim.E}27, in the presence of Dcr-2Scer\UAS.cDa, results in lethality.
Expression via P{cry-GAL4.Z}17 (in the presence of Dcr-2Scer\UAS.cDa) results in robust reduction in the the number of flies showing rhythmic behaviour in constant darkness and a concomitant reduction in the 'rhythmic strength' value of flies classified as rhythmic.
Adults expressing IscUGD14694 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.