FlyBase Allele Report: Dmel\Chi[E]

General Information

Symbol

Dmel\Chi^E

Species

D. melanogaster

Name

FlyBase ID

FBal0124370

Feature type

allele

Associated gene

Dmel\Chi

Associated Insertion(s)

Carried in Construct

Also Known As

Chip^E

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: R504W.

(Ramain et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:24,032,028..24,032,028 [-]

Nucleotide change:

C24032028T

Amino acid change:

R504W | Chi-PA; R523W | Chi-PB

Reported amino acid change:

R504W

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ramain et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Biryukova and Heitzler, 2005, Ramain et al., 2000)

visible

(Asmar et al., 2008)

visible | dominant

(de Navascués and Modolell, 2010)

visible | recessive

(de Navascués and Modolell, 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous Chi^E mutants display supernumerary scutellar bristles and, compared to wild-type, decreased number of dorsocentral bristles.

Chi^E heterozygotes display supernumerary scutellar bristles.

(de Navascués and Modolell, 2010)

Homozygotes show loss of dorsocentral bristles and have a slight thoracic cleft. The wings are filled by hemolymph between the dorsal and ventral layers.

(Asmar et al., 2008)

Chi^E flies show a dominant excess of scutellar bristle phenotype and a recessive loss of dorsocentral sensory organs. Homozygotes also exhibit a mild thoracic cleft.

(Biryukova and Heitzler, 2005)

Chi^E heterozygotes exhibit 1.6 dorsocentral bristles per heminotum compared to 2.0 in wild-type controls.

(Vanolst et al., 2005)

Chi^E mutant adults show a thoracic cleft and loss of dorsocentral bristles.

(Heitzler et al., 2003)

Chi^E shows a dominant excess of scutellar bristles. Homozygotes show a mild cleft along the dorsal midline of the thorax together with loss of the dorsocentral and posterior postalar bristles.

(Ramain et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Chi^E has visible phenotype, enhanceable by Bx^D110

(Asmar et al., 2008)

NOT suppressed by

Statement

Reference

Chi^E has visible phenotype, non-suppressible by Bx^hdp-n60

(Asmar et al., 2008)

Chi^E has visible | adult stage phenotype, non-suppressible by Bx^hdp58-1

(Zenvirt et al., 2008)

Chi^E has visible | adult stage phenotype, non-suppressible by Bx^hdp185-1

(Zenvirt et al., 2008)

Chi^E has visible | adult stage phenotype, non-suppressible by Bx^hdpR590

(Zenvirt et al., 2008)

Chi^E has visible | adult stage phenotype, non-suppressible by Bx²

(Zenvirt et al., 2008)

Chi^E has visible | adult stage phenotype, non-suppressible by Bx¹⁰⁰²

(Zenvirt et al., 2008)

Enhancer of

Statement

Reference

Chi^E is an enhancer of visible phenotype of Scer\GAL4^pnr-MD237, sc^A, sc^UAS.cPa

(de Navascués and Modolell, 2010)

Other

Statement

Reference

Chi^E, tup^isl-1/tup[+] has lethal phenotype

(Biryukova and Heitzler, 2005)

Chi^E, pnr[+]/pnr^VE12 has lethal phenotype

(Biryukova and Heitzler, 2005)

Chi^E, pnr^MD237 has partially lethal - majority die phenotype

(Ramain et al., 2000)

Chi^E, pnr^VX6 has lethal phenotype

(Ramain et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Chi^E has dorsocentral bristle phenotype, enhanceable by Bx^D110

(Asmar et al., 2008)

Chi^E has adult thorax phenotype, enhanceable by Bx^D110

(Asmar et al., 2008)

Chi^E has phenotype, enhanceable by ush[+]/ush^VE27

(Biryukova and Heitzler, 2008)

Chi^E has thorax phenotype, enhanceable by tup^E20

(Biryukova and Heitzler, 2005)

Chi^E has scutellar bristle | increased number phenotype, enhanceable by tup^E20

(Biryukova and Heitzler, 2005)

Chi^E has thorax phenotype, enhanceable by tup¹/tup[+]

(Biryukova and Heitzler, 2005)

Chi^E has scutellar bristle | increased number phenotype, enhanceable by tup¹/tup[+]

(Biryukova and Heitzler, 2005)

Chi^E has thorax phenotype, enhanceable by tup[+]/tup²

(Biryukova and Heitzler, 2005)

Chi^E has scutellar bristle | increased number phenotype, enhanceable by tup[+]/tup²

(Biryukova and Heitzler, 2005)

Chi^E has thorax phenotype, enhanceable by tup^isl-1/tup[+]

(Biryukova and Heitzler, 2005)

Chi^E has scutellar bristle | increased number phenotype, enhanceable by tup^isl-1/tup[+]

(Biryukova and Heitzler, 2005)

Chi^E has thorax phenotype, enhanceable by tup^E52/tup[+]

(Biryukova and Heitzler, 2005)

Chi^E has scutellar bristle | increased number phenotype, enhanceable by tup^E52/tup[+]

(Biryukova and Heitzler, 2005)

Chi^E has thorax phenotype, enhanceable by tup^E53/tup[+]

(Biryukova and Heitzler, 2005)

Chi^E has scutellar bristle | increased number phenotype, enhanceable by tup^E53/tup[+]

(Biryukova and Heitzler, 2005)

Chi^E has thorax phenotype, enhanceable by tup[+]/tup^E55

(Biryukova and Heitzler, 2005)

Chi^E has scutellar bristle | increased number phenotype, enhanceable by tup[+]/tup^E55

(Biryukova and Heitzler, 2005)

Chi^E has thorax phenotype, enhanceable by tup^E115/tup[+]

(Biryukova and Heitzler, 2005)

Chi^E has scutellar bristle | increased number phenotype, enhanceable by tup^E115/tup[+]

(Biryukova and Heitzler, 2005)

Chi^E has thorax phenotype, enhanceable by tup[+]/tup^2L-57-40

(Biryukova and Heitzler, 2005)

Chi^E has scutellar bristle | increased number phenotype, enhanceable by tup[+]/tup^2L-57-40

(Biryukova and Heitzler, 2005)

Chi^E has dorsocentral bristle phenotype, enhanceable by tou²

(Vanolst et al., 2005)

Chi^E has dorsocentral bristle phenotype, enhanceable by Iswi²

(Vanolst et al., 2005)

Chi^E has thorax phenotype, enhanceable by brm²

(Heitzler et al., 2003)

Chi^E has thorax phenotype, enhanceable by osa^4H

(Heitzler et al., 2003)

Chi^E has thorax phenotype, enhanceable by osa⁶¹⁶

(Heitzler et al., 2003)

Chi^E has thorax phenotype, enhanceable by osa^E17

(Heitzler et al., 2003)

Chi^E has thorax phenotype, enhanceable by pnr^MD237

(Ramain et al., 2000)

Chi^E has thorax phenotype, enhanceable by pnr^VX6

(Ramain et al., 2000)

Chi^E has scutellar bristle | ectopic phenotype, enhanceable by Tp(2;Y)A151

(Ramain et al., 2000)

Chi^E has thorax phenotype, enhanceable by Tp(2;Y)A151

(Ramain et al., 2000)

NOT Enhanced by

Statement

Reference

Chi^E has dorsocentral bristle phenotype, non-enhanceable by mor¹

(Heitzler et al., 2003)

Chi^E has thorax phenotype, non-enhanceable by mor¹

(Heitzler et al., 2003)

Suppressed by

Statement

Reference

Chi^E has scutellar bristle | increased number phenotype, suppressible by tup^UAS.cTa/Scer\GAL4^pnr-MD237

(Biryukova and Heitzler, 2005)

Chi^E has dorsocentral bristle phenotype, suppressible by brm²

(Heitzler et al., 2003)

Chi^E has dorsocentral bristle phenotype, suppressible by osa^4H

(Heitzler et al., 2003)

Chi^E has dorsocentral bristle phenotype, suppressible by osa⁶¹⁶

(Heitzler et al., 2003)

Chi^E has dorsocentral bristle phenotype, suppressible by osa^E17

(Heitzler et al., 2003)

Chi^E has scutellar bristle | ectopic phenotype, suppressible by ap^P44

(Ramain et al., 2000)

Chi^E has scutellar bristle | ectopic phenotype, suppressible by ap^Su(ChipE)

(Ramain et al., 2000)

Chi^E has scutellar bristle | ectopic phenotype, suppressible by ap^UGO35

(Ramain et al., 2000)

Chi^E has thorax phenotype, suppressible by ap^P44

(Ramain et al., 2000)

Chi^E has thorax phenotype, suppressible by ap^Su(ChipE)

(Ramain et al., 2000)

Chi^E has thorax phenotype, suppressible by ap^UGO35

(Ramain et al., 2000)

Chi^E has thorax phenotype, suppressible by pnr^UAS.cHa/Scer\GAL4^pnr-MD237

(Ramain et al., 2000)

NOT suppressed by

Statement

Reference

Chi^E has dorsocentral bristle phenotype, non-suppressible by Bx^hdp-n60

(Asmar et al., 2008)

Chi^E has adult thorax phenotype, non-suppressible by Bx^hdp-n60

(Asmar et al., 2008)

Chi^E has anterior dorsocentral bristle phenotype, non-suppressible by Bx¹⁰⁰²

(Zenvirt et al., 2008)

Chi^E has anterior dorsocentral bristle phenotype, non-suppressible by Bx^hdp58-1

(Zenvirt et al., 2008)

Chi^E has anterior dorsocentral bristle phenotype, non-suppressible by Bx^hdp185-1

(Zenvirt et al., 2008)

Chi^E has anterior dorsocentral bristle phenotype, non-suppressible by Bx^hdpR590

(Zenvirt et al., 2008)

Chi^E has anterior dorsocentral bristle phenotype, non-suppressible by Bx²

(Zenvirt et al., 2008)

Chi^E has dorsocentral bristle phenotype, non-suppressible by mor¹

(Heitzler et al., 2003)

Chi^E has thorax phenotype, non-suppressible by mor¹

(Heitzler et al., 2003)

Chi^E has dorsocentral bristle phenotype, non-suppressible by pnr^UAS.cHa/Scer\GAL4^pnr-MD237

(Ramain et al., 2000)

Enhancer of

Statement

Reference

Chi^E is an enhancer of scutellar bristle | increased number phenotype of Scer\GAL4^pnr-MD237, sc^A, sc^UAS.cPa

(de Navascués and Modolell, 2010)

Chi^E is an enhancer of dorsocentral bristle phenotype of tou²

(Vanolst et al., 2005)

Other

Statement

Reference

Chi^E, Scer\GAL4^ap-md544, tou^EP622 has dorsocentral bristle phenotype

(Vanolst et al., 2005)

Chi^E, pnr^VX6 has thorax phenotype

(Ramain et al., 2000)

Chi[+]/Chi^E, pnr^VX6 has thorax phenotype

(Ramain et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Roughly twice as many bristles are generated when sc^Scer\UAS.cPa is overexpressed under the control of Scer\GAL4^pnr-MD237 in a homozygous sc^A Chi^E double mutant background compared with that in a homozygous sc^A single mutant genetic background.

(de Navascués and Modolell, 2010)

The Chi^E homozygous phenotype of loss of dorsocentral bristles and presence of a slight thoracic cleft is not modified by hemizygosity for Bx^hdp-n60.

The Chi^E homozygous phenotype of loss of dorsocentral bristles and presence of a slight thoracic cleft is enhanced by hemizygosity for Bx^D110.

The mutant wing phenotypes of Bx^D110 and Chi^E are mutually suppressed in Bx^D110/+ ; Chi^E flies.

(Asmar et al., 2008)

Bx^hdp58-1 does not suppress the anterior dorsocentral bristle loss seen in Chi^E/+ mutant flies.

Bx^hdp185-1 does not suppress the anterior dorsocentral bristle loss seen in Chi^E/+ mutant flies.

Bx^hdpR590 does not suppress the anterior dorsocentral bristle loss seen in Chi^E/+ mutant flies.

Bx² does not suppress the anterior dorsocentral bristle loss seen in Chi^E/+ mutant flies.

Bx¹⁰⁰² does not suppress the anterior dorsocentral bristle loss seen in Chi^E/+ mutant flies.

(Zenvirt et al., 2008)

The following tup alleles enhance the excess scutellar bristle and thoracic cleft phenotype of Chi^E flies when heterozygous: tup¹, tup², tup^isl-1, tup^E52, tup^E53, tup^E55, tup^E115 and tup^2L-57-40. tup^E20 enhances the Chi^E phenotype when it is hemizygous or homozygous.

Expression of tup^Scer\UAS.cTa, under the control of Scer\GAL4^ap-md544, in a Chi^E background causes no wing defects.

Expression of tup^Scer\UAS.cTa, under the control of Scer\GAL4^pnr-MD237, partially suppresses the excess scutellar bristle phenotype of Chi^E mutants.

Although Chi^E flies are viable, Chi^E, tup^isl-1/+ and Chi^E; pnr^VE12/+ double mutant flies are lethal.

(Biryukova and Heitzler, 2005)

tou² Chi^E flies exhibit 1.11 dorsocentral bristles per heminotum compared to 2.0 in wild-type controls. Expression of tou^EP622, under the control of Scer\GAL4^ap-md544 in Chi^E homozygous flies results in suppression of the dorsocentral bristle loss observed in Chi^E mutants. Double mutants exhibit an average of 2.23 dorsocentral bristles per heminotum compared to 2.0 in wild-type. Iswi²/+ ; Chi^E/+ flies display on average 1.29 dorsocentral bristles per heminotum, so accentuating the pnr^D1/+ single mutant phenotype (1.6 per heminotum).

(Vanolst et al., 2005)

The addition of osa^E17, osa⁶¹⁶ or osa^4H enhances the thoracic cleft phenotype but suppresses the loss of dorsocentral bristles. The addition of brm² enhances the thoracic cleft phenotype but suppresses the loss of dorsocentral bristles.

(Heitzler et al., 2003)

The addition of pnr^VX6/+ to heterozygous Chi^E flies gives flies that exhibit a thoracic cleft. The addition of pnr^VX6/+ to homozygous Chi^E flies leads to lethality. The addition of pnr^MD237/+ to homozygous Chi^E flies produces a semi-lethal phenotype. Escapers exhibit a strong thoracic cleft phenotype. The addition of overexpressed pnr^Scer\UAS.cHa (when driven by Scer\GAL4^pnr-MD237) suppresses the cleft phenotype of homozygous Chi^E mutants, though the loss of dorsocentral bristles is not rescued. The addition of ap^UGO35 or ap^P44 or ap^Su(ChipE) to Chi^E homozygotes suppresses the thoracic left and loss of bristle phenotype seen in these flies.

(Ramain et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Chi^k04405

(Ramain et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36527

w¹¹¹⁸; Chi^E

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Chi^E

(de Navascués and Modolell, 2010)

Chip^E

(Asmar et al., 2008, Biryukova and Heitzler, 2008, Zenvirt et al., 2008, Heitzler, 2006.8.9, Biryukova and Heitzler, 2005, Vanolst et al., 2005, Heitzler et al., 2003)

Chip^Euc

(Zenvirt et al., 2008)

Name Synonyms

ChipE

(de Navascués and Modolell, 2010, Asmar et al., 2007)

Secondary FlyBase IDs

References (10)

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