From: Pascal Heitzler <pascal@XXXX>
To: Ruth Seal <rs468@XXXX>
Date: Wed, 9 Aug 2006  11:51:03  +0200
Subject: Re: pnr[VE12]
pnr[VE12] and pnr[VE13] require a little communication (Schaal, 
Biryukova & Heitzler) to flybase since they did not appear in 
previous literature.
They were EMS induced and represent new null alleles of pnr, found in 
a Chip[E]/+ background as dominant enhancers of Chip[E]. In both cases 
they encode a severely truncated protein due to premature stop codon.
VE12: R98*. R98 (cga) becomes stop (tga). VE12 encodes a 97 aa isoform A 
protein and a 45 aa isoform B protein. VE13: Q113*. Q113 (cag) becomes stop (tag). VE13 encodes a 112 aa isoform A protein and a 60 aa isoform B protein.  The molecular characterization has been performed (pcr) by Aurelie Schaal during her DUT probationary period in my lab. Both mutants show the severe embryonic dorsal hole phenotype.