FlyBase Allele Report: Dmel\tup[UAS.cTa]

General Information

Symbol

Dmel\tup^UAS.cTa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Thor

FlyBase ID

FBal0060384

Feature type

allele

Associated gene

Dmel\tup

Associated Insertion(s)

Carried in Construct

P{UAS-isl.T}

Also Known As

UAS-tup, UAS-isl

Key Links

Transgenic product class

inframe_deletion

(Thor and Thomas, 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Thor and Thomas, 1997)

Mutagen

in vitro construct

(Thor and Thomas, 1997)

Progenitor genotype

Carried in construct

P{UAS-isl.T}

Cytology

Description

A truncated tup cDNA (nucleotides 425 to 2982) is expressed under the control of UAS regulatory sequences.

(Thor and Thomas, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tup

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^sca-537.4

(Thor and Thomas, 1997)

abnormal neurophysiology, with Scer\GAL4^how-24B

(Wolfram et al., 2014)

visible, with Scer\GAL4^C-765

(de Navascués and Modolell, 2007)

visible, with Scer\GAL4^ey.PH

(Roignant et al., 2010)

Phenotype Manifest In

dorsocentral bristle, with Scer\GAL4^pnr-MD237

(Biryukova and Heitzler, 2005)

eye, with Scer\GAL4^ey.PH

(Roignant et al., 2010)

femur, with Scer\GAL4^dpp.blk1

(Pueyo and Couso, 2004)

interneuron | embryonic stage, with Scer\GAL4^sca-537.4

(Thor and Thomas, 1997)

mesothoracic pleurum, with Scer\GAL4^C-765

(de Navascués and Modolell, 2007)

metatarsus, with Scer\GAL4^dpp.blk1

(Pueyo and Couso, 2004)

motor neuron | embryonic stage, with Scer\GAL4^sca-537.4

(Thor and Thomas, 1997)

muscle cell of A1-7 dorsal acute muscle 2 | ectopic, with Scer\GAL4^twi.PB

(Boukhatmi et al., 2012)

muscle cell of A1-7 dorsal acute muscle 3, with Scer\GAL4^twi.PB

(Boukhatmi et al., 2012)

scutellar bristle, with Scer\GAL4^pnr-MD237

(Biryukova and Heitzler, 2005)

tarsal segment 4, with Scer\GAL4^ap-md544

(Pueyo and Couso, 2004)

tegula, with Scer\GAL4^C-765

(de Navascués and Modolell, 2007)

thorax, with Scer\GAL4^pnr-MD237

(Biryukova and Heitzler, 2005)

tibia, with Scer\GAL4^dpp.blk1

(Pueyo and Couso, 2004)

unguis, with Scer\GAL4^dpp.blk1

(Pueyo and Couso, 2004)

wing, with Scer\GAL4^ap-md544

(Biryukova and Heitzler, 2005)

Detailed Description

Statement

Reference

Expression of tup^Scer\UAS.cTa under the control of Scer\GAL4^twi.2PE does not induce alary muscle duplications in embryos.

(Boukhatmi et al., 2014)

Expression of tup^Scer\UAS.cTa in body wall muscle (under the control of Scer\GAL4^how-24B) reduces the magnitude of K[+] fast currents.

(Wolfram et al., 2014)

Embryos expressing tup^Scer\UAS.cTa under the control of Scer\GAL4^twi.PB show a transformation of muscle DA3 to DA2 in 88% of segments.

(Boukhatmi et al., 2012)

Mis-expression of tup^Scer\UAS.cTa under the control of Scer\GAL4^ey.PH reduces or abolishes the eye.

(Roignant et al., 2010)

Expression of tup^Scer\UAS.cTa under the control of Scer\GAL4^C-765 results in the formation of notum-like tissue in the mesothoracic pleura and extra notum-like bristles on the tegula.

(de Navascués and Modolell, 2007)

Expression of tup^Scer\UAS.cTa, under the control of Scer\GAL4^pnr-MD237, affects the development of the dorsocentral and scutellar bristles and induces a thoracic cleft.

Expression of tup^Scer\UAS.cTa, under the control of Scer\GAL4^ap-md544, results in the formation of rudimentary wings.

(Biryukova and Heitzler, 2005)

The legs of tup^Scer\UAS.cTa; Scer\GAL4^ap-md544 flies lack a fourth tarsal segment. tup^Scer\UAS.cTa; Scer\GAL4^dpp.blk1 flies the femur, tibia and first tarsal segment are fused. In the pretarsus only one claw develops.

(Pueyo and Couso, 2004)

tup^isl-1 mutants expressing tup^Scer\UAS.cTa using Scer\GAL4^elav.PLu do not hatch. Produces changes in axonal projections of interneurons and motor neurons, when expressed using Scer\GAL4^sca-537.4. Motor neurons that normally innervate dorsal muscles often remain within the ventral muscle region. Both ISN and SNa motor axons often fail to reach their normal target areas and show abnormal arborisations over the ventral muscle region.

(Thor and Thomas, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^how-24B, tup^UAS.cTa has abnormal neurophysiology phenotype, enhanceable by Lim3^UAS.cTa, Scer\GAL4^how-24B

(Wolfram et al., 2014)

Suppressor of

Statement

Reference

tup^UAS.cTa, Scer\GAL4^CCAP.PP is a suppressor of abnormal eclosion phenotype of Bx^EP1306, Scer\GAL4^CCAP.PP

(Hari et al., 2008)

Other

Statement

Reference

Scer\GAL4^C-765, ara^UAS.cGa, tup^UAS.cTa has visible phenotype

(de Navascués and Modolell, 2007)

Scer\GAL4^dpp.blk1, ara^UAS.cGa, tup^UAS.cTa has visible phenotype

(de Navascués and Modolell, 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, tup^UAS.cTa has wing phenotype, suppressible by ap^UAS.cOa/Scer\GAL4^ap-md544

(Biryukova and Heitzler, 2005)

Scer\GAL4^ap-md544, tup^UAS.cTa has tarsal segment 4 phenotype, suppressible | partially by Bar^UAS.cKa, Scer\GAL4^ap-md544

(Pueyo and Couso, 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^ap-md544, tup^UAS.cTa has tarsal segment 4 phenotype, non-suppressible by Chi^{FL.UAS.Tag:MYC}, Scer\GAL4^ap-md544

(Pueyo and Couso, 2004)

Scer\GAL4^ap-md544, tup^UAS.cTa has tarsal segment 4 phenotype, non-suppressible by ap^UAS.cOa/Scer\GAL4^ap-md544

(Pueyo and Couso, 2004)

Suppressor of

Statement

Reference

tup^UAS.cTa, Scer\GAL4^CCAP.PP is a suppressor of wing phenotype of Bx^EP1306, Scer\GAL4^CCAP.PP

(Hari et al., 2008)

tup^UAS.cTa/Scer\GAL4^pnr-MD237 is a suppressor of scutellar bristle | increased number phenotype of Chi^E

(Biryukova and Heitzler, 2005)

Other

Statement

Reference

Scer\GAL4^C-765, ara^UAS.cGa, tup^UAS.cTa has wing phenotype

(de Navascués and Modolell, 2007)

Scer\GAL4^C-765, ara^UAS.cGa, tup^UAS.cTa has wing hinge phenotype

(de Navascués and Modolell, 2007)

Scer\GAL4^dpp.blk1, ara^UAS.cGa, tup^UAS.cTa has wing phenotype

(de Navascués and Modolell, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Lim3^Scer\UAS.cTa enhances the reduction in K[+] fast currents found upon expression of tup^Scer\UAS.cTa under the control of Scer\GAL4^how-24B.

(Wolfram et al., 2014)

Expression of tup^Scer\UAS.cTa with Bx^EP1306, both under the control of Scer\GAL4^Ccap.PP, suppresses the wing expansion defect found upon expression of Bx^EP1306 alone.

(Hari et al., 2008)

Co-expression of both tup^Scer\UAS.cTa and ara^Scer\UAS.cGa under the control of Scer\GAL4^C-765 results in the wing and wing hinge being replaced by a large structure of notum-like tissue.

Co-expression of both tup^Scer\UAS.cTa and ara^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 transforms the central part of the wing to notum-like tissue, whereas the anterior and posterior parts develop as wing tissue. The animals also show notum-like structures near the coxa, indicating transformation of the ventral region of the body wall towards notum.

(de Navascués and Modolell, 2007)

Coexpression of the ap^Scer\UAS.cOa transgene rescues the rudimentary wing phenotype of Scer\GAL4^ap-md544>tup^Scer\UAS.cTa flies.

Expression of tup^Scer\UAS.cTa, under the control of Scer\GAL4^ap-md544, in a Chi^E background causes no wing defects.

Expression of tup^Scer\UAS.cTa, under the control of Scer\GAL4^pnr-MD237, partially suppresses the excess scutellar bristle phenotype of Chi^E mutants.

(Biryukova and Heitzler, 2005)

The loss of tarsal segment 4 in the legs of tup^Scer\UAS.cTa; Scer\GAL4^ap-md544 flies is not suppressed by ap^Scer\UAS.cOa or Chi^{FL.Scer\UAS.T:Hsap\MYC}, but is partially suppressed by B^Scer\UAS.cKa.

(Pueyo and Couso, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

39662

w^*; P{UAS-isl.T}10

39660

P{UAS-isl.T}16F, w^*

39661

w^*; P{UAS-isl.T}4M/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

isl^UAS.cTa

tup^Scer\UAS.cTa

tup^UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Thor

(Thor and Thomas, 1997)

Secondary FlyBase IDs

References (13)

Report Sections

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